Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries
Most biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to the identification of a large numb...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1159434/full |
| _version_ | 1797847637350678528 |
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| author | Faizan Ahmed Sadiq Koen De Reu Mette Burmølle Sharon Maes Marc Heyndrickx Marc Heyndrickx |
| author_facet | Faizan Ahmed Sadiq Koen De Reu Mette Burmølle Sharon Maes Marc Heyndrickx Marc Heyndrickx |
| author_sort | Faizan Ahmed Sadiq |
| collection | DOAJ |
| description | Most biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to the identification of a large number (327) of highly diverse bacterial species on food contact surfaces of the dairy, meat, and egg industries after routine cleaning and disinfection (C&D) regimes. In the current study, biofilm-forming ability of 92 bacterial strains belonging to 26 genera and 42 species was assessed and synergistic interactions in biofilm formation were investigated by coculturing species in all possible four-species combinations. Out of the total 455 four-species biofilm combinations, greater biofilm mass production, compared to the sum of biofilm masses of individual species in monoculture, was observed in 34 combinations. Around half of the combinations showed synergy in biofilm mass > 1.5-fold and most of the combinations belonged to dairy strains. The highest synergy (3.13-fold) was shown by a combination of dairy strains comprising Stenotrophomonas rhizophila, Bacillus licheniformis, Microbacterium lacticum, and Calidifontibacter indicus. The observed synergy in mixed biofilms turned out to be strain-specific rather than species-dependent. All biofilm combinations showing remarkable synergy appeared to have certain common species in all combinations which shows there are keystone industry-specific bacterial species which stimulate synergy or antagonism and this may have implication for biofilm control in the concerned food industries. |
| first_indexed | 2024-04-09T18:15:46Z |
| format | Article |
| id | doaj.art-829b3231e1b84574bd8975344ce7b06d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:15:46Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-829b3231e1b84574bd8975344ce7b06d2023-04-13T04:37:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11594341159434Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industriesFaizan Ahmed Sadiq0Koen De Reu1Mette Burmølle2Sharon Maes3Marc Heyndrickx4Marc Heyndrickx5Technology and Food Science Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, BelgiumTechnology and Food Science Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, BelgiumSection of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, DenmarkThe Department of Ecotechnology and Sustainable Building Engineering, Mid Sweden University, Östersund, SwedenTechnology and Food Science Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, BelgiumDepartment of Pathobiology, Pharmacology and Zoological Medicine, Ghent University, Merelbeke, BelgiumMost biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to the identification of a large number (327) of highly diverse bacterial species on food contact surfaces of the dairy, meat, and egg industries after routine cleaning and disinfection (C&D) regimes. In the current study, biofilm-forming ability of 92 bacterial strains belonging to 26 genera and 42 species was assessed and synergistic interactions in biofilm formation were investigated by coculturing species in all possible four-species combinations. Out of the total 455 four-species biofilm combinations, greater biofilm mass production, compared to the sum of biofilm masses of individual species in monoculture, was observed in 34 combinations. Around half of the combinations showed synergy in biofilm mass > 1.5-fold and most of the combinations belonged to dairy strains. The highest synergy (3.13-fold) was shown by a combination of dairy strains comprising Stenotrophomonas rhizophila, Bacillus licheniformis, Microbacterium lacticum, and Calidifontibacter indicus. The observed synergy in mixed biofilms turned out to be strain-specific rather than species-dependent. All biofilm combinations showing remarkable synergy appeared to have certain common species in all combinations which shows there are keystone industry-specific bacterial species which stimulate synergy or antagonism and this may have implication for biofilm control in the concerned food industries.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1159434/fullbacterial interactionssynergydairy industrybiofilmsdisinfection |
| spellingShingle | Faizan Ahmed Sadiq Koen De Reu Mette Burmølle Sharon Maes Marc Heyndrickx Marc Heyndrickx Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries Frontiers in Microbiology bacterial interactions synergy dairy industry biofilms disinfection |
| title | Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries |
| title_full | Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries |
| title_fullStr | Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries |
| title_full_unstemmed | Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries |
| title_short | Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries |
| title_sort | synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries |
| topic | bacterial interactions synergy dairy industry biofilms disinfection |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1159434/full |
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