Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota
Abstract Background Obesity is a severe public health threat worldwide. Emerging evidence suggests that gut microbiota dysbiosis is closely associated with obesity and its related metabolic complications. The aim of the present study is to investigate the effects of polysaccharide extracted from WuG...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Nutrition & Metabolism |
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| Online Access: | http://link.springer.com/article/10.1186/s12986-020-00442-2 |
| _version_ | 1818312265004220416 |
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| author | Wendong Wang Mintao Zhong Tiantian Yu Lei Chen Lijun Shi Junwei Zong Shouyu Wang |
| author_facet | Wendong Wang Mintao Zhong Tiantian Yu Lei Chen Lijun Shi Junwei Zong Shouyu Wang |
| author_sort | Wendong Wang |
| collection | DOAJ |
| description | Abstract Background Obesity is a severe public health threat worldwide. Emerging evidence suggests that gut microbiota dysbiosis is closely associated with obesity and its related metabolic complications. The aim of the present study is to investigate the effects of polysaccharide extracted from WuGuChong (PEW) on high-fat diet (HFD)-induced obesity, and the potential mechanisms involving modulation of the gut microbiota composition. Methods Mice were fed a normal chow diet and a high-fat diet with or without PEW (300 mg/kg/day) by oral gavage for 8 weeks. Body weight, obesity-related metabolic disorders, and gut microbiota were examined at the end of the experiment. Results PEW supplementation reduces body weight, adipose hypertrophy, liver steatosis, insulin resistance and systemic inflammation in HFD-fed mice, as well as maintains intestinal epithelium integrity. High-throughput 16S rRNA analysis demonstrates that PEW supplementation alters the composition of gut microbiota. The Firmicutes to Bacteroidetes ratio and the relative abundance of Proteobacteria are increased in HFD-fed mice, which are reversed by PEW supplementation to approximately the control levels. Conclusions Our results suggest that PEW may be used as a bioactive ingredient to prevent obesity and its related metabolic disorders by modulating the composition of gut microbiota. |
| first_indexed | 2024-12-13T08:15:06Z |
| format | Article |
| id | doaj.art-829ddf208abd492fa2332ae0b9b68176 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-12-13T08:15:06Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-829ddf208abd492fa2332ae0b9b681762022-12-21T23:54:07ZengBMCNutrition & Metabolism1743-70752020-03-0117111210.1186/s12986-020-00442-2Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiotaWendong Wang0Mintao Zhong1Tiantian Yu2Lei Chen3Lijun Shi4Junwei Zong5Shouyu Wang6Department of Orthopedic Surgery, The First Affiliated Hospital of Dalian Medical UniversityDepartment of Microbiology, Dalian Medical UniversityDepartment of Gynaecology and Obstetrics, Dalian Municipal Woman and Children’s Medical CenterDepartment of Microbiology, Dalian Medical UniversityDepartment of Orthopedic Surgery, The First Affiliated Hospital of Dalian Medical UniversityDepartment of Orthopedic Surgery, The First Affiliated Hospital of Dalian Medical UniversityDepartment of Orthopedic Surgery, The First Affiliated Hospital of Dalian Medical UniversityAbstract Background Obesity is a severe public health threat worldwide. Emerging evidence suggests that gut microbiota dysbiosis is closely associated with obesity and its related metabolic complications. The aim of the present study is to investigate the effects of polysaccharide extracted from WuGuChong (PEW) on high-fat diet (HFD)-induced obesity, and the potential mechanisms involving modulation of the gut microbiota composition. Methods Mice were fed a normal chow diet and a high-fat diet with or without PEW (300 mg/kg/day) by oral gavage for 8 weeks. Body weight, obesity-related metabolic disorders, and gut microbiota were examined at the end of the experiment. Results PEW supplementation reduces body weight, adipose hypertrophy, liver steatosis, insulin resistance and systemic inflammation in HFD-fed mice, as well as maintains intestinal epithelium integrity. High-throughput 16S rRNA analysis demonstrates that PEW supplementation alters the composition of gut microbiota. The Firmicutes to Bacteroidetes ratio and the relative abundance of Proteobacteria are increased in HFD-fed mice, which are reversed by PEW supplementation to approximately the control levels. Conclusions Our results suggest that PEW may be used as a bioactive ingredient to prevent obesity and its related metabolic disorders by modulating the composition of gut microbiota.http://link.springer.com/article/10.1186/s12986-020-00442-2Gut microbiotaHigh-fat dietObesityPolysaccharidesWuGuChong |
| spellingShingle | Wendong Wang Mintao Zhong Tiantian Yu Lei Chen Lijun Shi Junwei Zong Shouyu Wang Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota Nutrition & Metabolism Gut microbiota High-fat diet Obesity Polysaccharides WuGuChong |
| title | Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota |
| title_full | Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota |
| title_fullStr | Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota |
| title_full_unstemmed | Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota |
| title_short | Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota |
| title_sort | polysaccharide extracted from wuguchong reduces high fat diet induced obesity in mice by regulating the composition of intestinal microbiota |
| topic | Gut microbiota High-fat diet Obesity Polysaccharides WuGuChong |
| url | http://link.springer.com/article/10.1186/s12986-020-00442-2 |
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