Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscle
Summary Guanylyl cyclases mediate a number of physiological processes, including smooth muscle function and axonal guidance. Here, we report a novel role for Drosophila receptor-type guanylyl cyclase at 76C, Gyc76C, in development of the embryonic somatic muscle. In embryos lacking function of Gyc76...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2012-04-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/1/6/507 |
| _version_ | 1818646797617201152 |
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| author | Unisha Patel Shireen A. Davies Monn Monn Myat |
| author_facet | Unisha Patel Shireen A. Davies Monn Monn Myat |
| author_sort | Unisha Patel |
| collection | DOAJ |
| description | Summary
Guanylyl cyclases mediate a number of physiological processes, including smooth muscle function and axonal guidance. Here, we report a novel role for Drosophila receptor-type guanylyl cyclase at 76C, Gyc76C, in development of the embryonic somatic muscle. In embryos lacking function of Gyc76C or the downstream cGMP-dependent protein kinase (cGK), DG1, patterning of the somatic body wall muscles was abnormal with ventral and lateral muscle groups showing the most severe defects. In contrast, specification and elongation of the dorsal oblique and dorsal acute muscles of gyc76C mutant embryos was normal, and instead, these muscles showed defects in proper formation of the myotendinous junctions (MTJs). During MTJ formation in gyc76C and pkg21D mutant embryos, the βPS integrin subunit failed to localize to the MTJs and instead was found in discrete puncta within the myotubes. Tissue-specific rescue experiments showed that gyc76C function is required in the muscle for proper patterning and βPS integrin localization at the MTJ. These studies provide the first evidence for a requirement for Gyc76C and DG1 in Drosophila somatic muscle development, and suggest a role in transport and/or retention of integrin receptor subunits at the developing MTJs. |
| first_indexed | 2024-12-17T00:52:21Z |
| format | Article |
| id | doaj.art-829e19bd351142f69fa4cabfea78d4d2 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T00:52:21Z |
| publishDate | 2012-04-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-829e19bd351142f69fa4cabfea78d4d22022-12-21T22:09:44ZengThe Company of BiologistsBiology Open2046-63902012-04-011650751510.1242/bio.20129432012943Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscleUnisha Patel0Shireen A. Davies1Monn Monn Myat2 Department of Cell and Developmental Biology, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK Department of Cell and Developmental Biology, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA Summary Guanylyl cyclases mediate a number of physiological processes, including smooth muscle function and axonal guidance. Here, we report a novel role for Drosophila receptor-type guanylyl cyclase at 76C, Gyc76C, in development of the embryonic somatic muscle. In embryos lacking function of Gyc76C or the downstream cGMP-dependent protein kinase (cGK), DG1, patterning of the somatic body wall muscles was abnormal with ventral and lateral muscle groups showing the most severe defects. In contrast, specification and elongation of the dorsal oblique and dorsal acute muscles of gyc76C mutant embryos was normal, and instead, these muscles showed defects in proper formation of the myotendinous junctions (MTJs). During MTJ formation in gyc76C and pkg21D mutant embryos, the βPS integrin subunit failed to localize to the MTJs and instead was found in discrete puncta within the myotubes. Tissue-specific rescue experiments showed that gyc76C function is required in the muscle for proper patterning and βPS integrin localization at the MTJ. These studies provide the first evidence for a requirement for Gyc76C and DG1 in Drosophila somatic muscle development, and suggest a role in transport and/or retention of integrin receptor subunits at the developing MTJs.http://bio.biologists.org/content/1/6/507Guanylyl cyclaseDrosophilaMuscleIntegrinAdhesion |
| spellingShingle | Unisha Patel Shireen A. Davies Monn Monn Myat Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscle Biology Open Guanylyl cyclase Drosophila Muscle Integrin Adhesion |
| title | Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscle |
| title_full | Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscle |
| title_fullStr | Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscle |
| title_full_unstemmed | Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscle |
| title_short | Receptor-type guanylyl cyclase Gyc76C is required for development of the Drosophila embryonic somatic muscle |
| title_sort | receptor type guanylyl cyclase gyc76c is required for development of the drosophila embryonic somatic muscle |
| topic | Guanylyl cyclase Drosophila Muscle Integrin Adhesion |
| url | http://bio.biologists.org/content/1/6/507 |
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