A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra
ObjectiveThe aim of this study was to explore the lipidomic profiles of the CAL-27 human tongue cancer cell line and the human oral keratinocyte (HOK) cell line.MethodsThe lipidomic differences between the CAL-27 and the HOK cell lines were investigated using non-targeted high-performance liquid chr...
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.771337/full |
| _version_ | 1819320253694345216 |
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| author | Xue-ying Wang Ting Zhang Wei-qun Guan Hua-zhu Li Ling Lin |
| author_facet | Xue-ying Wang Ting Zhang Wei-qun Guan Hua-zhu Li Ling Lin |
| author_sort | Xue-ying Wang |
| collection | DOAJ |
| description | ObjectiveThe aim of this study was to explore the lipidomic profiles of the CAL-27 human tongue cancer cell line and the human oral keratinocyte (HOK) cell line.MethodsThe lipidomic differences between the CAL-27 and the HOK cell lines were investigated using non-targeted high-performance liquid chromatography–mass spectrometry lipidomic analysis. The resulting data were then further mined via bioinformatics analysis technology and metabolic pathway analysis was conducted in order to map the most affected metabolites and pathways in the two cell lines.ResultsA total of 711 lipids were identified, including 403 glycerophospholipids (GPs), 147 glycerolipids, and 161 sphingolipids. Comparison of the enhanced MS (EMS) spectra of the two cell lines in positive and negative ionization modes showed the lipid compositions of HOK and CAL-27 cells to be similar. The expressions of most GP species in CAL-27 cells showed an increasing trend as compared with HOK, whereas a significant increase in phosphatidylcholine was observed (p < 0.05). Significant differences in the lipid composition between CAL-27 and HOK cells were shown as a heatmap. Through principal component analysis and orthogonal partial least squares discriminant analysis, noticeably clear separation trends and satisfactory clustering trends between groups of HOK and CAL-27 cells were identified. The numbers of specific lipid metabolites that could distinguish CAL-27 from HOK in positive and negative modes were 100 and 248, respectively. GP metabolism was the most significantly altered lipid metabolic pathway, with 4 metabolites differentially expressed in 39 hit products.ConclusionThis study demonstrated the potential of using untargeted mass spectra and bioinformatics analysis to describe the lipid profiles of HOK and CAL-27 cells. |
| first_indexed | 2024-12-24T11:16:39Z |
| format | Article |
| id | doaj.art-829e28c62c5548099e1644a87d8ad7a1 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-24T11:16:39Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-829e28c62c5548099e1644a87d8ad7a12022-12-21T16:58:21ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-12-011110.3389/fonc.2021.771337771337A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS SpectraXue-ying Wang0Ting Zhang1Wei-qun Guan2Hua-zhu Li3Ling Lin4Department of Stomatology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Stomatology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Stomatology, Fujian Medical University Union Hospital, Fuzhou, ChinaGeneral Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaInstitutes of Biomedical Sciences of Shanghai Medical School, Fudan University, Shanghai, ChinaObjectiveThe aim of this study was to explore the lipidomic profiles of the CAL-27 human tongue cancer cell line and the human oral keratinocyte (HOK) cell line.MethodsThe lipidomic differences between the CAL-27 and the HOK cell lines were investigated using non-targeted high-performance liquid chromatography–mass spectrometry lipidomic analysis. The resulting data were then further mined via bioinformatics analysis technology and metabolic pathway analysis was conducted in order to map the most affected metabolites and pathways in the two cell lines.ResultsA total of 711 lipids were identified, including 403 glycerophospholipids (GPs), 147 glycerolipids, and 161 sphingolipids. Comparison of the enhanced MS (EMS) spectra of the two cell lines in positive and negative ionization modes showed the lipid compositions of HOK and CAL-27 cells to be similar. The expressions of most GP species in CAL-27 cells showed an increasing trend as compared with HOK, whereas a significant increase in phosphatidylcholine was observed (p < 0.05). Significant differences in the lipid composition between CAL-27 and HOK cells were shown as a heatmap. Through principal component analysis and orthogonal partial least squares discriminant analysis, noticeably clear separation trends and satisfactory clustering trends between groups of HOK and CAL-27 cells were identified. The numbers of specific lipid metabolites that could distinguish CAL-27 from HOK in positive and negative modes were 100 and 248, respectively. GP metabolism was the most significantly altered lipid metabolic pathway, with 4 metabolites differentially expressed in 39 hit products.ConclusionThis study demonstrated the potential of using untargeted mass spectra and bioinformatics analysis to describe the lipid profiles of HOK and CAL-27 cells.https://www.frontiersin.org/articles/10.3389/fonc.2021.771337/fulllipidomic profileCAL-27HOKcell lineshuman tongue cancer |
| spellingShingle | Xue-ying Wang Ting Zhang Wei-qun Guan Hua-zhu Li Ling Lin A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra Frontiers in Oncology lipidomic profile CAL-27 HOK cell lines human tongue cancer |
| title | A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra |
| title_full | A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra |
| title_fullStr | A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra |
| title_full_unstemmed | A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra |
| title_short | A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra |
| title_sort | study of the lipidomic profiles of the cal 27 and hok cell lines using ems spectra |
| topic | lipidomic profile CAL-27 HOK cell lines human tongue cancer |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.771337/full |
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