Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome
Organic and microbial fertilizers have potential advantages over inorganic fertilizers in improving soil fertility and crop yield without harmful side-effects. However, the effects of these bio-organic fertilizers on the soil microbiome and metabolome remain largely unknown, especially in the contex...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1117355/full |
| _version_ | 1797924654026850304 |
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| author | Shangmeng Li Shangmeng Li Wei Fan Wei Fan Gang Xu Gang Xu Ying Cao Ying Cao Xin Zhao Xin Zhao Suwei Hao Suwei Hao Bin Deng Bin Deng Siyuan Ren Siyuan Ren Shanglian Hu Shanglian Hu |
| author_facet | Shangmeng Li Shangmeng Li Wei Fan Wei Fan Gang Xu Gang Xu Ying Cao Ying Cao Xin Zhao Xin Zhao Suwei Hao Suwei Hao Bin Deng Bin Deng Siyuan Ren Siyuan Ren Shanglian Hu Shanglian Hu |
| author_sort | Shangmeng Li |
| collection | DOAJ |
| description | Organic and microbial fertilizers have potential advantages over inorganic fertilizers in improving soil fertility and crop yield without harmful side-effects. However, the effects of these bio-organic fertilizers on the soil microbiome and metabolome remain largely unknown, especially in the context of bamboo cultivation. In this study, we cultivated Dendrocalamus farinosus (D. farinosus) plants under five different fertilization conditions: organic fertilizer (OF), Bacillus amyloliquefaciens bio-fertilizer (Ba), Bacillus mucilaginosus Krassilnikov bio-fertilizer (BmK), organic fertilizer plus Bacillus amyloliquefaciens bio-fertilizer (OFBa), and organic fertilizer plus Bacillus mucilaginosus Krassilnikov bio-fertilizer (OFBmK). We conducted 16S rRNA sequencing and liquid chromatography/mass spectrometry (LC–MS) to evaluate the soil bacterial composition and soil metabolic activity in the different treatment groups. The results demonstrate that all the fertilization conditions altered the soil bacterial community composition. Moreover, the combination of organic and microbial fertilizers (i.e., in the OFBa and OFBmK groups) significantly affected the relative abundance of soil bacterial species; the largest number of dominant microbial communities were found in the OFBa group, which were strongly correlated with each other. Additionally, non-targeted metabolomics revealed that the levels of soil lipids and lipid-like molecules, and organic acids and their derivatives, were greatly altered under all treatment conditions. The levels of galactitol, guanine, and deoxycytidine were also markedly decreased in the OFBa and OFBmK groups. Moreover, we constructed a regulatory network to delineated the relationships between bamboo phenotype, soil enzymatic activity, soil differential metabolites, and dominant microbial. The network revealed that bio-organic fertilizers promoted bamboo growth by modifying the soil microbiome and metabolome. Accordingly, we concluded that the use of organic fertilizers, microbial fertilizers, or their combination regulated bacterial composition and soil metabolic processes. These findings provide new insights into how D. farinosus-bacterial interactions are affected by different fertilization regiments, which are directly applicable to the agricultural cultivation of bamboo. |
| first_indexed | 2024-04-10T15:04:17Z |
| format | Article |
| id | doaj.art-829e5309351c4899b7de3232060ed306 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T15:04:17Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-829e5309351c4899b7de3232060ed3062023-02-15T06:41:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.11173551117355Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolomeShangmeng Li0Shangmeng Li1Wei Fan2Wei Fan3Gang Xu4Gang Xu5Ying Cao6Ying Cao7Xin Zhao8Xin Zhao9Suwei Hao10Suwei Hao11Bin Deng12Bin Deng13Siyuan Ren14Siyuan Ren15Shanglian Hu16Shanglian Hu17Lab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, ChinaEngineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, ChinaOrganic and microbial fertilizers have potential advantages over inorganic fertilizers in improving soil fertility and crop yield without harmful side-effects. However, the effects of these bio-organic fertilizers on the soil microbiome and metabolome remain largely unknown, especially in the context of bamboo cultivation. In this study, we cultivated Dendrocalamus farinosus (D. farinosus) plants under five different fertilization conditions: organic fertilizer (OF), Bacillus amyloliquefaciens bio-fertilizer (Ba), Bacillus mucilaginosus Krassilnikov bio-fertilizer (BmK), organic fertilizer plus Bacillus amyloliquefaciens bio-fertilizer (OFBa), and organic fertilizer plus Bacillus mucilaginosus Krassilnikov bio-fertilizer (OFBmK). We conducted 16S rRNA sequencing and liquid chromatography/mass spectrometry (LC–MS) to evaluate the soil bacterial composition and soil metabolic activity in the different treatment groups. The results demonstrate that all the fertilization conditions altered the soil bacterial community composition. Moreover, the combination of organic and microbial fertilizers (i.e., in the OFBa and OFBmK groups) significantly affected the relative abundance of soil bacterial species; the largest number of dominant microbial communities were found in the OFBa group, which were strongly correlated with each other. Additionally, non-targeted metabolomics revealed that the levels of soil lipids and lipid-like molecules, and organic acids and their derivatives, were greatly altered under all treatment conditions. The levels of galactitol, guanine, and deoxycytidine were also markedly decreased in the OFBa and OFBmK groups. Moreover, we constructed a regulatory network to delineated the relationships between bamboo phenotype, soil enzymatic activity, soil differential metabolites, and dominant microbial. The network revealed that bio-organic fertilizers promoted bamboo growth by modifying the soil microbiome and metabolome. Accordingly, we concluded that the use of organic fertilizers, microbial fertilizers, or their combination regulated bacterial composition and soil metabolic processes. These findings provide new insights into how D. farinosus-bacterial interactions are affected by different fertilization regiments, which are directly applicable to the agricultural cultivation of bamboo.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1117355/fullDendrocalamus farinosusorganic and microbial fertilizersfertilizationsoil microbiomebacterial communitysoil metabolome |
| spellingShingle | Shangmeng Li Shangmeng Li Wei Fan Wei Fan Gang Xu Gang Xu Ying Cao Ying Cao Xin Zhao Xin Zhao Suwei Hao Suwei Hao Bin Deng Bin Deng Siyuan Ren Siyuan Ren Shanglian Hu Shanglian Hu Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome Frontiers in Microbiology Dendrocalamus farinosus organic and microbial fertilizers fertilization soil microbiome bacterial community soil metabolome |
| title | Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome |
| title_full | Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome |
| title_fullStr | Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome |
| title_full_unstemmed | Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome |
| title_short | Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome |
| title_sort | bio organic fertilizers improve dendrocalamus farinosus growth by remolding the soil microbiome and metabolome |
| topic | Dendrocalamus farinosus organic and microbial fertilizers fertilization soil microbiome bacterial community soil metabolome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1117355/full |
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