Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo
Objective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV. Materials and methods: A total of 150 samples including animal...
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| Format: | Article |
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Network for the Veterinarians of Bangladesh
2017-12-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=276591 |
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| author | Bwihangane Ahadi Birindwa Gitao Chege George Bisimwa Patrick Ntagereka Okafor Christopher Bebora Caroline Lilly |
| author_facet | Bwihangane Ahadi Birindwa Gitao Chege George Bisimwa Patrick Ntagereka Okafor Christopher Bebora Caroline Lilly |
| author_sort | Bwihangane Ahadi Birindwa |
| collection | DOAJ |
| description | Objective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV.
Materials and methods: A total of 150 samples including animals swabs, tissues and blood were collected from unvaccinated goats in a PPR and/or Capripox outbreaks in South Kivu, Eastern of Democratic Republic of the Congo. Conventional PCR and reverse transcriptase (RT-PCR) were used respectively to amplify P32, RPO30, GPCR genes of Capripox virus and Fusion (F) protein of PPRV. Positive samples were sequenced for phylogenetic analysis.
Results: Out of 150 tested animals, 64.7% (n=97/150) were PPRV positive, 52.7% (n=79/150) were Capripox positive and 38.7% (n=58/150) were positive for both PPRV and CaPV. The pairwise comparison of P32 gene of CaPV and F gene of PPRV showed 99.75% of identity percentage among goatpox virus sequences, 96.95% among PPRV sequences and 47.91% between CaPV and PPRV sequences.
Conclusion: The study has demonstrated high prevalence of CaP V-PPRV mixed infection in South Kivu. Lumpy skin virus disease (LSVD) is a lineage circulating which has a genetic relationship between its P32 gene and the F gene of PPRV giving the challenge to differentiate the two diseases at the clinical farm level. [J Adv Vet Anim Res 2017; 4(4.000): 348-355] |
| first_indexed | 2024-12-11T12:22:31Z |
| format | Article |
| id | doaj.art-82a4f099d15340f4a7b93793161e56e5 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-11T12:22:31Z |
| publishDate | 2017-12-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-82a4f099d15340f4a7b93793161e56e52022-12-22T01:07:30ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102017-12-014434835510.5455/javar.2017.d233276591Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of CongoBwihangane Ahadi Birindwa0Gitao Chege George1Bisimwa Patrick Ntagereka2Okafor Christopher3Bebora Caroline Lilly4Department of Animal Science, Agriculture and Environmental faculty, Universite Evangelique en Afrique , PO Box 3323 Bukavu, Democratic Republic of the Congo, and Department of Veterinary Pathology, Microbiology & Parasitology, Faculty of Veterinary Medicine, University of Nairobi, PO Box 29053-00625,Uthiru, Kenya Department of Veterinary Pathology, Microbiology & Parasitology, Faculty of Veterinary Medicine, University of Nairobi, PO Box 29053-00625,Uthiru, Kenya Department of Animal Science, Agriculture and Environmental faculty, Universite Evangelique en Afrique , PO Box 3323 Bukavu, Democratic Republic of the Congo International Institute of Tropical Agriculture (IITA) Hub, Bukavu, Democratic Republic of the Congo Department of Veterinary Pathology, Microbiology & Parasitology, Faculty of Veterinary Medicine, University of Nairobi, PO Box 29053-00625,Uthiru, Kenya.Objective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV. Materials and methods: A total of 150 samples including animals swabs, tissues and blood were collected from unvaccinated goats in a PPR and/or Capripox outbreaks in South Kivu, Eastern of Democratic Republic of the Congo. Conventional PCR and reverse transcriptase (RT-PCR) were used respectively to amplify P32, RPO30, GPCR genes of Capripox virus and Fusion (F) protein of PPRV. Positive samples were sequenced for phylogenetic analysis. Results: Out of 150 tested animals, 64.7% (n=97/150) were PPRV positive, 52.7% (n=79/150) were Capripox positive and 38.7% (n=58/150) were positive for both PPRV and CaPV. The pairwise comparison of P32 gene of CaPV and F gene of PPRV showed 99.75% of identity percentage among goatpox virus sequences, 96.95% among PPRV sequences and 47.91% between CaPV and PPRV sequences. Conclusion: The study has demonstrated high prevalence of CaP V-PPRV mixed infection in South Kivu. Lumpy skin virus disease (LSVD) is a lineage circulating which has a genetic relationship between its P32 gene and the F gene of PPRV giving the challenge to differentiate the two diseases at the clinical farm level. [J Adv Vet Anim Res 2017; 4(4.000): 348-355]http://www.ejmanager.com/fulltextpdf.php?mno=276591Capripox; Coinfection; DRCongo; Goat; Phylogeny; PPRV |
| spellingShingle | Bwihangane Ahadi Birindwa Gitao Chege George Bisimwa Patrick Ntagereka Okafor Christopher Bebora Caroline Lilly Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo Journal of Advanced Veterinary and Animal Research Capripox; Coinfection; DRCongo; Goat; Phylogeny; PPRV |
| title | Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo |
| title_full | Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo |
| title_fullStr | Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo |
| title_full_unstemmed | Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo |
| title_short | Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo |
| title_sort | mixed infection of peste des petits ruminants and capripox in goats in south kivu democratic republic of congo |
| topic | Capripox; Coinfection; DRCongo; Goat; Phylogeny; PPRV |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=276591 |
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