MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of Glucocorticoids
An increase in the percentage of monocytes with reduced HLA-DR expression and immunosuppressive properties has been reported in numerous human neoplastic diseases, including lymphoma. However, there are no analogous studies on phenotypical variations in the peripheral blood monocytes in dogs with ly...
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MDPI AG
2022-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/16/2135 |
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| author | Alicja Rzepecka Dariusz Jagielski Anna Cywińska Rafał Sapierzyński Magdalena Żmigrodzka Olga Witkowska-Piłaszewicz Anna Winnicka |
| author_facet | Alicja Rzepecka Dariusz Jagielski Anna Cywińska Rafał Sapierzyński Magdalena Żmigrodzka Olga Witkowska-Piłaszewicz Anna Winnicka |
| author_sort | Alicja Rzepecka |
| collection | DOAJ |
| description | An increase in the percentage of monocytes with reduced HLA-DR expression and immunosuppressive properties has been reported in numerous human neoplastic diseases, including lymphoma. However, there are no analogous studies on phenotypical variations in the peripheral blood monocytes in dogs with lymphoma. The aim of this study was to determine the difference in the expression of the MHCII molecule on peripheral blood monocytes in dogs with lymphoma before any treatment (NRG) and in dogs that had previously received glucocorticoids (RG) in comparison to healthy dogs. Flow cytometry immunophenotyping of peripheral blood leukocytes was performed using canine-specific or cross-reactive antibodies against CD11b, CD14 and MHCII. In the blood of dogs with lymphoma (NRG and RG), compared to that of healthy ones, the MHCII+ and MHCII− monocytes ratio was changed due to an increase in the percentage of MHCII− monocytes. The number of MHCII− monocytes was significantly higher only in RG dogs compared to healthy ones, which might result from the release of these cells from the blood marginal pool due to the action of glucocorticoids. Our results encourage further studies to assess if changes in MHCII expression affect immune status in dogs with lymphoma. |
| first_indexed | 2024-03-09T04:48:02Z |
| format | Article |
| id | doaj.art-82a5863cc80143b8959ac730de9400f1 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T04:48:02Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-82a5863cc80143b8959ac730de9400f12023-12-03T13:13:46ZengMDPI AGAnimals2076-26152022-08-011216213510.3390/ani12162135MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of GlucocorticoidsAlicja Rzepecka0Dariusz Jagielski1Anna Cywińska2Rafał Sapierzyński3Magdalena Żmigrodzka4Olga Witkowska-Piłaszewicz5Anna Winnicka6Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, PolandVeterinary Surgery “Białobrzeska”, 02-344 Warsaw, PolandFaculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Toruń, PolandInstitute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, PolandInstitute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, PolandInstitute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, PolandInstitute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, PolandAn increase in the percentage of monocytes with reduced HLA-DR expression and immunosuppressive properties has been reported in numerous human neoplastic diseases, including lymphoma. However, there are no analogous studies on phenotypical variations in the peripheral blood monocytes in dogs with lymphoma. The aim of this study was to determine the difference in the expression of the MHCII molecule on peripheral blood monocytes in dogs with lymphoma before any treatment (NRG) and in dogs that had previously received glucocorticoids (RG) in comparison to healthy dogs. Flow cytometry immunophenotyping of peripheral blood leukocytes was performed using canine-specific or cross-reactive antibodies against CD11b, CD14 and MHCII. In the blood of dogs with lymphoma (NRG and RG), compared to that of healthy ones, the MHCII+ and MHCII− monocytes ratio was changed due to an increase in the percentage of MHCII− monocytes. The number of MHCII− monocytes was significantly higher only in RG dogs compared to healthy ones, which might result from the release of these cells from the blood marginal pool due to the action of glucocorticoids. Our results encourage further studies to assess if changes in MHCII expression affect immune status in dogs with lymphoma.https://www.mdpi.com/2076-2615/12/16/2135canine lymphomaflow cytometryglucocorticoidsimmunosuppressionmonocytes |
| spellingShingle | Alicja Rzepecka Dariusz Jagielski Anna Cywińska Rafał Sapierzyński Magdalena Żmigrodzka Olga Witkowska-Piłaszewicz Anna Winnicka MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of Glucocorticoids Animals canine lymphoma flow cytometry glucocorticoids immunosuppression monocytes |
| title | MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of Glucocorticoids |
| title_full | MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of Glucocorticoids |
| title_fullStr | MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of Glucocorticoids |
| title_full_unstemmed | MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of Glucocorticoids |
| title_short | MHCII Expression on Peripheral Blood Monocytes in Canine Lymphoma: An Impact of Glucocorticoids |
| title_sort | mhcii expression on peripheral blood monocytes in canine lymphoma an impact of glucocorticoids |
| topic | canine lymphoma flow cytometry glucocorticoids immunosuppression monocytes |
| url | https://www.mdpi.com/2076-2615/12/16/2135 |
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