Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus
Objectives: Autoantibodies and aberrant immune complexes are pathological hallmarks of systemic lupus erythematosus (SLE). This study aimed to determine the occurrence of IgG autoantibodies against human serum albumin (anti-HSA IgG) and their potential association with antibodies against bovine seru...
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| Format: | Article |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.02090/full |
| _version_ | 1818882290214764544 |
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| author | Josephine Nehring Lucia A. Schirmbeck Justa Friebus-Kardash Denise Dubler Uyen Huynh-Do Carlo Chizzolini Camillo Ribi Marten Trendelenburg |
| author_facet | Josephine Nehring Lucia A. Schirmbeck Justa Friebus-Kardash Denise Dubler Uyen Huynh-Do Carlo Chizzolini Camillo Ribi Marten Trendelenburg |
| author_sort | Josephine Nehring |
| collection | DOAJ |
| description | Objectives: Autoantibodies and aberrant immune complexes are pathological hallmarks of systemic lupus erythematosus (SLE). This study aimed to determine the occurrence of IgG autoantibodies against human serum albumin (anti-HSA IgG) and their potential association with antibodies against bovine serum albumin (anti-BSA IgG) in patients with SLE.Methods: Sera of 180 SLE patients included to the Swiss SLE Cohort Study and 188 age- and sex-matched healthy controls were evaluated. Levels of anti-HSA IgG and anti-BSA IgG were quantified by ELISA. Selected samples were further characterized using serum fractions obtained by fast liquid chromatography (FPLC).Results: SLE patients had increased levels of anti-HSA IgG (p = 0.002) but similar levels of anti-BSA IgG compared to matched healthy controls. Anti-HSA IgG levels correlated with the SLE Disease Activity Index (SLEDAI), which was more pronounced in patients with an physician's global assessment (PGA) of ≥ 1 (r = 0.309, p = 0.0066). Anti-HSA IgG was partially complexed with serum albumin but also occurred as monomeric autoantibodies in highly positive SLE patients. A positive correlation between anti-HSA IgG and anti-BSA IgG was found that was stronger in SLE patients than in healthy controls (r = 0.3172, p < 0.001 vs. r = 0.2122, p < 0.0035). Binding of anti-BSA IgG was inhibited partially in the presence of HSA in samples with double positivity for anti-HSA and anti-BSA (median inhibition 47.9%, range 0.9–100%) and vice versa.Conclusion: In SLE patients there is an increased prevalence of anti-HSA IgG antibodies that are associated with SLE disease activity. |
| first_indexed | 2024-12-19T15:15:24Z |
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| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T15:15:24Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-82a78f71a3db4a5f85a559333b654c6a2022-12-21T20:16:09ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-10-01910.3389/fimmu.2018.02090372940Autoantibodies Against Albumin in Patients With Systemic Lupus ErythematosusJosephine Nehring0Lucia A. Schirmbeck1Justa Friebus-Kardash2Denise Dubler3Uyen Huynh-Do4Carlo Chizzolini5Camillo Ribi6Marten Trendelenburg7Division of Internal Medicine and Clinical Immunology laboratory, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, SwitzerlandDivision of Internal Medicine and Clinical Immunology laboratory, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, SwitzerlandDepartment of Nephrology, University Hospital, Essen, GermanyDepartment of Biomedicine, University of Basel, Basel, SwitzerlandNephrology and Hypertension, University Hospital Bern, Bern, SwitzerlandInternal Medicine Specialties, Clinical Immunology and Allergy, Geneva University Hospitals, Geneva, SwitzerlandDepartment of Immunology and Allergy, Lausanne University Hospital, Lausanne, SwitzerlandDivision of Internal Medicine and Clinical Immunology laboratory, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, SwitzerlandObjectives: Autoantibodies and aberrant immune complexes are pathological hallmarks of systemic lupus erythematosus (SLE). This study aimed to determine the occurrence of IgG autoantibodies against human serum albumin (anti-HSA IgG) and their potential association with antibodies against bovine serum albumin (anti-BSA IgG) in patients with SLE.Methods: Sera of 180 SLE patients included to the Swiss SLE Cohort Study and 188 age- and sex-matched healthy controls were evaluated. Levels of anti-HSA IgG and anti-BSA IgG were quantified by ELISA. Selected samples were further characterized using serum fractions obtained by fast liquid chromatography (FPLC).Results: SLE patients had increased levels of anti-HSA IgG (p = 0.002) but similar levels of anti-BSA IgG compared to matched healthy controls. Anti-HSA IgG levels correlated with the SLE Disease Activity Index (SLEDAI), which was more pronounced in patients with an physician's global assessment (PGA) of ≥ 1 (r = 0.309, p = 0.0066). Anti-HSA IgG was partially complexed with serum albumin but also occurred as monomeric autoantibodies in highly positive SLE patients. A positive correlation between anti-HSA IgG and anti-BSA IgG was found that was stronger in SLE patients than in healthy controls (r = 0.3172, p < 0.001 vs. r = 0.2122, p < 0.0035). Binding of anti-BSA IgG was inhibited partially in the presence of HSA in samples with double positivity for anti-HSA and anti-BSA (median inhibition 47.9%, range 0.9–100%) and vice versa.Conclusion: In SLE patients there is an increased prevalence of anti-HSA IgG antibodies that are associated with SLE disease activity.https://www.frontiersin.org/article/10.3389/fimmu.2018.02090/fullantibodieshuman serum albuminpatientssystemic lupus erythematosusmulticenter study |
| spellingShingle | Josephine Nehring Lucia A. Schirmbeck Justa Friebus-Kardash Denise Dubler Uyen Huynh-Do Carlo Chizzolini Camillo Ribi Marten Trendelenburg Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus Frontiers in Immunology antibodies human serum albumin patients systemic lupus erythematosus multicenter study |
| title | Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus |
| title_full | Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus |
| title_fullStr | Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus |
| title_full_unstemmed | Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus |
| title_short | Autoantibodies Against Albumin in Patients With Systemic Lupus Erythematosus |
| title_sort | autoantibodies against albumin in patients with systemic lupus erythematosus |
| topic | antibodies human serum albumin patients systemic lupus erythematosus multicenter study |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.02090/full |
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