Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1042679/full |
| _version_ | 1798006400730791936 |
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| author | Jerko Hrabar Mirela Petrić Serena Cavallero Marco Salvemini Stefano D’Amelio Ivona Mladineo |
| author_facet | Jerko Hrabar Mirela Petrić Serena Cavallero Marco Salvemini Stefano D’Amelio Ivona Mladineo |
| author_sort | Jerko Hrabar |
| collection | DOAJ |
| description | Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestrial host, we stimulated peripheral blood leukocytes (PBLs) of fish (paratenic host) and rat (accidental, human-model host) with A. pegreffii crude extract and analysed PBL transcriptomes 1 and 12 h post-stimulation. Fish and rat PBLs differentially expressed 712 and 493 transcripts, respectively, between 1 and 12 h post-stimulation (false discovery rate, FDR <0.001, logFC >2). While there was a difference in the highest upregulated transcripts between two time-points, the same Gene Ontologies, biological processes (intracellular signal transduction, DNA-dependent transcription, and DNA-regulated regulation of transcription), and molecular functions (ATP and metal ion binding) were enriched in the two hosts, showing an incrementing dynamic between 1 and 12 h. This suggests that the two distinct hosts employ qualitatively different transcript cascades only to achieve the same effect, at least during an early innate immunity response. Activation of later immunity elements and/or a combination of other host’s intrinsic conditions may contribute to the death of L3 in the terrestrial host. |
| first_indexed | 2024-04-11T12:54:08Z |
| format | Article |
| id | doaj.art-82aa36b051634557bd61caf6755fa244 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T12:54:08Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-82aa36b051634557bd61caf6755fa2442022-12-22T04:23:06ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-12-011210.3389/fcimb.2022.10426791042679Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extractJerko Hrabar0Mirela Petrić1Serena Cavallero2Marco Salvemini3Stefano D’Amelio4Ivona Mladineo5Laboratory of Aquaculture, Institute of Oceanography and Fisheries, Split, CroatiaUniversity Department of Marine Studies, University of Split, Split, CroatiaDepartment of Public Health and Infectious Diseases, University of Rome, Sapienza, Rome, ItalyDepartment of Biology, University of Naples Federico II, Naples, ItalyDepartment of Public Health and Infectious Diseases, University of Rome, Sapienza, Rome, ItalyLaboratory of Functional Helminthology, Institute of Parasitology, Biology Centre of Czech Academy of Sciences, Ceske Budejovice, CzechiaInfective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestrial host, we stimulated peripheral blood leukocytes (PBLs) of fish (paratenic host) and rat (accidental, human-model host) with A. pegreffii crude extract and analysed PBL transcriptomes 1 and 12 h post-stimulation. Fish and rat PBLs differentially expressed 712 and 493 transcripts, respectively, between 1 and 12 h post-stimulation (false discovery rate, FDR <0.001, logFC >2). While there was a difference in the highest upregulated transcripts between two time-points, the same Gene Ontologies, biological processes (intracellular signal transduction, DNA-dependent transcription, and DNA-regulated regulation of transcription), and molecular functions (ATP and metal ion binding) were enriched in the two hosts, showing an incrementing dynamic between 1 and 12 h. This suggests that the two distinct hosts employ qualitatively different transcript cascades only to achieve the same effect, at least during an early innate immunity response. Activation of later immunity elements and/or a combination of other host’s intrinsic conditions may contribute to the death of L3 in the terrestrial host.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1042679/fullAnisakiscrude extractfishratperipheral blood leukocytesRNA-Seq |
| spellingShingle | Jerko Hrabar Mirela Petrić Serena Cavallero Marco Salvemini Stefano D’Amelio Ivona Mladineo Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract Frontiers in Cellular and Infection Microbiology Anisakis crude extract fish rat peripheral blood leukocytes RNA-Seq |
| title | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
| title_full | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
| title_fullStr | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
| title_full_unstemmed | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
| title_short | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
| title_sort | rat and fish peripheral blood leukocytes respond distinctively to anisakis pegreffii nematoda anisakidae crude extract |
| topic | Anisakis crude extract fish rat peripheral blood leukocytes RNA-Seq |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1042679/full |
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