Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol
Abstract Background Alcohol is one of the most commonly consumed neurotoxins by humans. Its negative effect on bone health is known for a long time. However, its impact on qualitative and quantitative 2D characteristics of the compact bone is still unclear. Therefore, the aim of this study was to in...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Aristotle University of Thessaloniki
2018-05-01
|
| Series: | Journal of Biological Research - Thessaloniki |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40709-018-0079-1 |
| _version_ | 1811193643057283072 |
|---|---|
| author | Monika Martiniakova Anna Sarocka Ramona Babosova Birgit Grosskopf Edyta Kapusta Zofia Goc Grzegorz Formicki Radoslav Omelka |
| author_facet | Monika Martiniakova Anna Sarocka Ramona Babosova Birgit Grosskopf Edyta Kapusta Zofia Goc Grzegorz Formicki Radoslav Omelka |
| author_sort | Monika Martiniakova |
| collection | DOAJ |
| description | Abstract Background Alcohol is one of the most commonly consumed neurotoxins by humans. Its negative effect on bone health is known for a long time. However, its impact on qualitative and quantitative 2D characteristics of the compact bone is still unclear. Therefore, the aim of this study was to investigate in detail the effects of subchronic alcohol exposure on compact and trabecular bone tissues microstructure of laboratory mice using 2D and 3D imaging methods. Ten clinically healthy 12 weeks-old mice (males) were randomly divided into two groups. Animals from experimental group (group E; n = 5) drank a solution composed of 15% ethanol and water (1.7 g 100% ethanol kg−1 b.w. per day) for 8 weeks, while those from control group (group C; n = 5) drank only water. Results Subchronic exposure to alcohol leads to several changes in qualitative 2D characteristics of the compact bone such as the presence of primary vascular radial bone tissue in pars anterior of endosteal border and a higher number of resorption lacunae (five times more) in the middle part of substantia compacta. Morphometrical 2D evaluations of the compact bone showed significantly increased sizes of primary osteons’ vascular canals (p < 0.05) in mice from the experimental group (E group). Sizes of Haversian canals and secondary osteons were not affected by alcohol consumption. In mice from the E group, significantly lower values for relative bone volume and bone mineral density of the compact bone were observed. In the trabecular bone, decreased values for bone volume, trabecular number, trabecular thickness and bone surface (p < 0.05) were documented. Conclusions Alcohol decreased not only bone volume and density of the compact bone, but it also reduced trabecular bone volume and leads to trabecular thinning. It caused vasodilation of primary osteons’ vascular canals and increased porosity in the compact bone. |
| first_indexed | 2024-04-12T00:11:30Z |
| format | Article |
| id | doaj.art-82ad4f3e4d804e1e856f917095e4f943 |
| institution | Directory Open Access Journal |
| issn | 2241-5793 |
| language | English |
| last_indexed | 2024-04-12T00:11:30Z |
| publishDate | 2018-05-01 |
| publisher | Aristotle University of Thessaloniki |
| record_format | Article |
| series | Journal of Biological Research - Thessaloniki |
| spelling | doaj.art-82ad4f3e4d804e1e856f917095e4f9432022-12-22T03:55:56ZengAristotle University of ThessalonikiJournal of Biological Research - Thessaloniki2241-57932018-05-012511710.1186/s40709-018-0079-1Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcoholMonika Martiniakova0Anna Sarocka1Ramona Babosova2Birgit Grosskopf3Edyta Kapusta4Zofia Goc5Grzegorz Formicki6Radoslav Omelka7Department of Zoology and Anthropology, Constantine the Philosopher University in NitraDepartment of Zoology and Anthropology, Constantine the Philosopher University in NitraDepartment of Zoology and Anthropology, Constantine the Philosopher University in NitraInstitute of Zoology and Anthropology, Georg-August UniversityDepartment of Animal Physiology and Toxicology, Pedagogical University of CracowDepartment of Animal Physiology and Toxicology, Pedagogical University of CracowDepartment of Animal Physiology and Toxicology, Pedagogical University of CracowDepartment of Botany and Genetics, Constantine the Philosopher University in NitraAbstract Background Alcohol is one of the most commonly consumed neurotoxins by humans. Its negative effect on bone health is known for a long time. However, its impact on qualitative and quantitative 2D characteristics of the compact bone is still unclear. Therefore, the aim of this study was to investigate in detail the effects of subchronic alcohol exposure on compact and trabecular bone tissues microstructure of laboratory mice using 2D and 3D imaging methods. Ten clinically healthy 12 weeks-old mice (males) were randomly divided into two groups. Animals from experimental group (group E; n = 5) drank a solution composed of 15% ethanol and water (1.7 g 100% ethanol kg−1 b.w. per day) for 8 weeks, while those from control group (group C; n = 5) drank only water. Results Subchronic exposure to alcohol leads to several changes in qualitative 2D characteristics of the compact bone such as the presence of primary vascular radial bone tissue in pars anterior of endosteal border and a higher number of resorption lacunae (five times more) in the middle part of substantia compacta. Morphometrical 2D evaluations of the compact bone showed significantly increased sizes of primary osteons’ vascular canals (p < 0.05) in mice from the experimental group (E group). Sizes of Haversian canals and secondary osteons were not affected by alcohol consumption. In mice from the E group, significantly lower values for relative bone volume and bone mineral density of the compact bone were observed. In the trabecular bone, decreased values for bone volume, trabecular number, trabecular thickness and bone surface (p < 0.05) were documented. Conclusions Alcohol decreased not only bone volume and density of the compact bone, but it also reduced trabecular bone volume and leads to trabecular thinning. It caused vasodilation of primary osteons’ vascular canals and increased porosity in the compact bone.http://link.springer.com/article/10.1186/s40709-018-0079-1AlcoholMicrostructureCompact boneTrabecular boneMice |
| spellingShingle | Monika Martiniakova Anna Sarocka Ramona Babosova Birgit Grosskopf Edyta Kapusta Zofia Goc Grzegorz Formicki Radoslav Omelka Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol Journal of Biological Research - Thessaloniki Alcohol Microstructure Compact bone Trabecular bone Mice |
| title | Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol |
| title_full | Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol |
| title_fullStr | Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol |
| title_full_unstemmed | Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol |
| title_short | Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol |
| title_sort | changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol |
| topic | Alcohol Microstructure Compact bone Trabecular bone Mice |
| url | http://link.springer.com/article/10.1186/s40709-018-0079-1 |
| work_keys_str_mv | AT monikamartiniakova changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol AT annasarocka changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol AT ramonababosova changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol AT birgitgrosskopf changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol AT edytakapusta changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol AT zofiagoc changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol AT grzegorzformicki changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol AT radoslavomelka changesinthemicrostructureofcompactandtrabecularbonetissuesofmicesubchronicallyexposedtoalcohol |