Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage
Background: Cancer is amongst the leading reasons of death in all parts of the world. Breast cancer is also responsible for the largest number of deaths among women population. Several studies confirmed that Bifidobacterium bifidum as a probiotic meaningfully inhibited breast cancer development. The...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2019-11-01
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| Series: | Research in Molecular Medicine |
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| Online Access: | http://rmm.mazums.ac.ir/article-1-334-en.html |
| _version_ | 1819007430029213696 |
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| author | Parisima Karami Saeid Abediankenari Hamid Reza Goli Mehrdad Gholami Mohammad Ahanjan |
| author_facet | Parisima Karami Saeid Abediankenari Hamid Reza Goli Mehrdad Gholami Mohammad Ahanjan |
| author_sort | Parisima Karami |
| collection | DOAJ |
| description | Background: Cancer is amongst the leading reasons of death in all parts of the world. Breast cancer is also responsible for the largest number of deaths among women population. Several studies confirmed that Bifidobacterium bifidum as a probiotic meaningfully inhibited breast cancer development. The present study aimed to investigate the effect of B. bifidum supernatant on the cell growth inhibition of the breast cancer 4T1 cell line, in vitro.
Materials and methods: The present experimental work was conducted at Mazandaran University of Medical Sciences (MAZUMS), Sari, Iran. B. bifidum was cultured in the MRS broth at 37°C for 72 hours anaerobically and the B. bifidum supernatant (BS) was prepared by the freezing-thawing procedure. The cell growth inhibition of the probiotic strain was assessed using the MTT assay through breast cancer (4T1) cell line.
Results: The results showed that the supernatant extracted from B.bifidum strain had good antiproliferative effects against 4T1 cancer cell line, compared to control group. The inhibitory effects growing with increased time.
Conclusion: B. bifidum supernatant could be considered as a as potential probiotic candidates in the treatment of breast cancer. However, further in vitro/in vivo studies are required to complement our initial findings. |
| first_indexed | 2024-12-21T00:24:27Z |
| format | Article |
| id | doaj.art-82ae1952f03940c5800f4f7ca09ce361 |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-12-21T00:24:27Z |
| publishDate | 2019-11-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-82ae1952f03940c5800f4f7ca09ce3612022-12-21T19:22:02ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2019-11-017416Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell LineageParisima Karami0Saeid Abediankenari1Hamid Reza Goli2Mehrdad Gholami3Mohammad Ahanjan4 Department of Microbiology and virology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Department of Microbiology and virology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Department of Microbiology and virology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Background: Cancer is amongst the leading reasons of death in all parts of the world. Breast cancer is also responsible for the largest number of deaths among women population. Several studies confirmed that Bifidobacterium bifidum as a probiotic meaningfully inhibited breast cancer development. The present study aimed to investigate the effect of B. bifidum supernatant on the cell growth inhibition of the breast cancer 4T1 cell line, in vitro. Materials and methods: The present experimental work was conducted at Mazandaran University of Medical Sciences (MAZUMS), Sari, Iran. B. bifidum was cultured in the MRS broth at 37°C for 72 hours anaerobically and the B. bifidum supernatant (BS) was prepared by the freezing-thawing procedure. The cell growth inhibition of the probiotic strain was assessed using the MTT assay through breast cancer (4T1) cell line. Results: The results showed that the supernatant extracted from B.bifidum strain had good antiproliferative effects against 4T1 cancer cell line, compared to control group. The inhibitory effects growing with increased time. Conclusion: B. bifidum supernatant could be considered as a as potential probiotic candidates in the treatment of breast cancer. However, further in vitro/in vivo studies are required to complement our initial findings.http://rmm.mazums.ac.ir/article-1-334-en.htmlbreast cancerbifidobacterium bifidum4t1 cell linemtt |
| spellingShingle | Parisima Karami Saeid Abediankenari Hamid Reza Goli Mehrdad Gholami Mohammad Ahanjan Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage Research in Molecular Medicine breast cancer bifidobacterium bifidum 4t1 cell line mtt |
| title | Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage |
| title_full | Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage |
| title_fullStr | Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage |
| title_full_unstemmed | Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage |
| title_short | Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage |
| title_sort | evaluation of cell growth inhibition of bifidobacterium bifidum cell free supernatant extract on 4t1tumor cell lineage |
| topic | breast cancer bifidobacterium bifidum 4t1 cell line mtt |
| url | http://rmm.mazums.ac.ir/article-1-334-en.html |
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