Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan
In our previous studies, the production of four bioactive molecules by Streptomyces sp. PAL114 in complex ISP2 broth medium has been described. Three of these molecules belong to the angucycline family. In this study, two novel antibiotics belonging to the same family were produced by strain PAL114...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Saudi Pharmaceutical Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S131901641930091X |
| _version_ | 1811327313597431808 |
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| author | Samira Tata Adel Aouiche Christian Bijani Noureddine Bouras Frédéric Pont Florence Mathieu Nasserdine Sabaou |
| author_facet | Samira Tata Adel Aouiche Christian Bijani Noureddine Bouras Frédéric Pont Florence Mathieu Nasserdine Sabaou |
| author_sort | Samira Tata |
| collection | DOAJ |
| description | In our previous studies, the production of four bioactive molecules by Streptomyces sp. PAL114 in complex ISP2 broth medium has been described. Three of these molecules belong to the angucycline family. In this study, two novel antibiotics belonging to the same family were produced by strain PAL114 on M2 synthetic medium containing L-tryptophan as precursor. These antibiotics, named mzabimycins A and B, were intracellular and produced only in the presence of L-tryptophan. After four days of culturing PAL114 in the M2 medium, the bioactive compounds were extracted from mycelium with methanol and then analyzed by HPLC on reverse phase C18 column. Two active purplish blue fractions were purified. The chemical structures of these molecules were determined on the basis of spectroscopic and spectrometric analyses (1H and 13C NMR, and mass spectra). They were identified to be novel angucycline derivative antibiotics. The pure molecules showed activity against some pathogenic Gram-positive bacteria which have multiple antibiotic resistance, such as Staphylococcus aureus MRSA 639c and Listeria monocytogenes ATCC 13932. Keywords: Antimicrobial compounds, Angucycline antibiotics, L-Tryptophan, Synthetic medium, Streptomyces |
| first_indexed | 2024-04-13T15:05:35Z |
| format | Article |
| id | doaj.art-82ae24b82ff443c6a0f7005c6ceb5e94 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-13T15:05:35Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-82ae24b82ff443c6a0f7005c6ceb5e942022-12-22T02:42:11ZengElsevierSaudi Pharmaceutical Journal1319-01642019-11-01277907913Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophanSamira Tata0Adel Aouiche1Christian Bijani2Noureddine Bouras3Frédéric Pont4Florence Mathieu5Nasserdine Sabaou6Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, AlgeriaLaboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, AlgeriaLaboratoire de Chimie de Coordination (LCC), CNRS, Université de Toulouse, UPS, INPT, LCC, 205 Route de Narbonne, 31077 Toulouse, FranceLaboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria; Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa 47000, AlgeriaProteomics Group, Centre de Recherches en Cancérologie de Toulouse (CRCT), INSERM UMR1037, Toulouse, FranceLaboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Corresponding authors at: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, B.P. 92, 16 050 Kouba, Algiers, Algeria.Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria; Corresponding authors at: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, B.P. 92, 16 050 Kouba, Algiers, Algeria.In our previous studies, the production of four bioactive molecules by Streptomyces sp. PAL114 in complex ISP2 broth medium has been described. Three of these molecules belong to the angucycline family. In this study, two novel antibiotics belonging to the same family were produced by strain PAL114 on M2 synthetic medium containing L-tryptophan as precursor. These antibiotics, named mzabimycins A and B, were intracellular and produced only in the presence of L-tryptophan. After four days of culturing PAL114 in the M2 medium, the bioactive compounds were extracted from mycelium with methanol and then analyzed by HPLC on reverse phase C18 column. Two active purplish blue fractions were purified. The chemical structures of these molecules were determined on the basis of spectroscopic and spectrometric analyses (1H and 13C NMR, and mass spectra). They were identified to be novel angucycline derivative antibiotics. The pure molecules showed activity against some pathogenic Gram-positive bacteria which have multiple antibiotic resistance, such as Staphylococcus aureus MRSA 639c and Listeria monocytogenes ATCC 13932. Keywords: Antimicrobial compounds, Angucycline antibiotics, L-Tryptophan, Synthetic medium, Streptomyceshttp://www.sciencedirect.com/science/article/pii/S131901641930091X |
| spellingShingle | Samira Tata Adel Aouiche Christian Bijani Noureddine Bouras Frédéric Pont Florence Mathieu Nasserdine Sabaou Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan Saudi Pharmaceutical Journal |
| title | Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan |
| title_full | Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan |
| title_fullStr | Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan |
| title_full_unstemmed | Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan |
| title_short | Mzabimycins A and B, novel intracellular angucycline antibiotics produced by Streptomyces sp. PAL114 in synthetic medium containing L-tryptophan |
| title_sort | mzabimycins a and b novel intracellular angucycline antibiotics produced by streptomyces sp pal114 in synthetic medium containing l tryptophan |
| url | http://www.sciencedirect.com/science/article/pii/S131901641930091X |
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