Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cells
AbstractPurpose Local tumor heating with microwave applicators has been used in multimodal breast cancer therapies. This hyperthermia allows to target small regions while marginally affecting healthy tissue. However, most preclinical examinations only use simplified heating methods. Microwave applic...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | International Journal of Hyperthermia |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/02656736.2023.2265590 |
| _version_ | 1797374230859350016 |
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| author | Jannik Walter Michael Hader Azzaya Sengedorj Rainer Fietkau Benjamin Frey Udo S. Gaipl |
| author_facet | Jannik Walter Michael Hader Azzaya Sengedorj Rainer Fietkau Benjamin Frey Udo S. Gaipl |
| author_sort | Jannik Walter |
| collection | DOAJ |
| description | AbstractPurpose Local tumor heating with microwave applicators has been used in multimodal breast cancer therapies. This hyperthermia allows to target small regions while marginally affecting healthy tissue. However, most preclinical examinations only use simplified heating methods. Microwave applicators employed for deep heating to provide the greatest depth of penetration operate in the tens to hundreds frequency. Therefore, we aimed to adapt and test a clinically often used broadband spiral applicator (105–125 MHz) for hyperthermia with clinically wanted temperatures of 41 and 44 °C in in vitro settings with human breast cancer cell lines and with simulations.Material and Methods A clinically used spiral-microwave applicator (105–125 MHz) was the basis for the construction, simulation, and optimization of the in vitro HT set-up under stationary conditions. Microwave effects on tumor cell death of two human breast cancer cell lines (hormone-receptor positive MCF-7 and triple-negative MDA-MB-231) were compared with conventional heating in a contact-heating chamber. Cell death forms were analyzed by AnnexinV/Propidium iodide staining.Results An in vitro spiral applicator microwave-based heating system that is effective at applying heat directly to adherent breast cancer cells in cell culture flasks with medium was developed. Simulations with COMSOL proved appropriate heat delivery and an optimal energy coupling at a frequency of 111 ± 2.5 MHz. Apoptosis and necrosis induction and significantly higher cell death rates than conventional heating at both temperatures were observed, and MCF-7 showed higher death rates than MDA-MB-231 tumor cells.Conclusions Well-characterized in vitro heating systems are mandatory for a better understanding of the biological effects of hyperthermia in tumor therapies and to finally determine optimized clinical treatment schemes. |
| first_indexed | 2024-03-08T19:02:55Z |
| format | Article |
| id | doaj.art-82b20aa651884642bc90cd37fcaa91a8 |
| institution | Directory Open Access Journal |
| issn | 0265-6736 1464-5157 |
| language | English |
| last_indexed | 2024-03-08T19:02:55Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Hyperthermia |
| spelling | doaj.art-82b20aa651884642bc90cd37fcaa91a82023-12-28T04:15:43ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572023-12-0140110.1080/02656736.2023.2265590Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cellsJannik Walter0Michael Hader1Azzaya Sengedorj2Rainer Fietkau3Benjamin Frey4Udo S. Gaipl5Translational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, GermanyTranslational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, GermanyTranslational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, GermanyDepartment of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, GermanyTranslational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, GermanyTranslational Radiobiology, Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, GermanyAbstractPurpose Local tumor heating with microwave applicators has been used in multimodal breast cancer therapies. This hyperthermia allows to target small regions while marginally affecting healthy tissue. However, most preclinical examinations only use simplified heating methods. Microwave applicators employed for deep heating to provide the greatest depth of penetration operate in the tens to hundreds frequency. Therefore, we aimed to adapt and test a clinically often used broadband spiral applicator (105–125 MHz) for hyperthermia with clinically wanted temperatures of 41 and 44 °C in in vitro settings with human breast cancer cell lines and with simulations.Material and Methods A clinically used spiral-microwave applicator (105–125 MHz) was the basis for the construction, simulation, and optimization of the in vitro HT set-up under stationary conditions. Microwave effects on tumor cell death of two human breast cancer cell lines (hormone-receptor positive MCF-7 and triple-negative MDA-MB-231) were compared with conventional heating in a contact-heating chamber. Cell death forms were analyzed by AnnexinV/Propidium iodide staining.Results An in vitro spiral applicator microwave-based heating system that is effective at applying heat directly to adherent breast cancer cells in cell culture flasks with medium was developed. Simulations with COMSOL proved appropriate heat delivery and an optimal energy coupling at a frequency of 111 ± 2.5 MHz. Apoptosis and necrosis induction and significantly higher cell death rates than conventional heating at both temperatures were observed, and MCF-7 showed higher death rates than MDA-MB-231 tumor cells.Conclusions Well-characterized in vitro heating systems are mandatory for a better understanding of the biological effects of hyperthermia in tumor therapies and to finally determine optimized clinical treatment schemes.https://www.tandfonline.com/doi/10.1080/02656736.2023.2265590Microwave hyperthermia105–125 MHz spiral applicatormicrowave frequencybreast cancercell death formsmultimodal tumor treatment |
| spellingShingle | Jannik Walter Michael Hader Azzaya Sengedorj Rainer Fietkau Benjamin Frey Udo S. Gaipl Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cells International Journal of Hyperthermia Microwave hyperthermia 105–125 MHz spiral applicator microwave frequency breast cancer cell death forms multimodal tumor treatment |
| title | Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cells |
| title_full | Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cells |
| title_fullStr | Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cells |
| title_full_unstemmed | Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cells |
| title_short | Broadband microwave spiral applicator (105–125 MHz) for in vitro examinations of hyperthermia-induced tumor cell death forms – first analyses with human breast cancer cells |
| title_sort | broadband microwave spiral applicator 105 125 mhz for in vitro examinations of hyperthermia induced tumor cell death forms first analyses with human breast cancer cells |
| topic | Microwave hyperthermia 105–125 MHz spiral applicator microwave frequency breast cancer cell death forms multimodal tumor treatment |
| url | https://www.tandfonline.com/doi/10.1080/02656736.2023.2265590 |
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