In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis
Remyelination is crucial to recover from inflammatory demyelination in multiple sclerosis (MS). Investigating remyelination in vivo using magnetic resonance imaging (MRI) is difficult in MS, where collecting serial short-interval scans is challenging. Using experimental autoimmune encephalomyelitis...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/73786 |
| _version_ | 1797829695384846336 |
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| author | Maxime Donadieu Nathanael J Lee María I Gaitán Seung-Kwon Ha Nicholas J Luciano Snehashis Roy Benjamin Ineichen Emily C Leibovitch Cecil C Yen Dzung L Pham Afonso C Silva Mac Johnson Steve Jacobson Pascal Sati Daniel S Reich |
| author_facet | Maxime Donadieu Nathanael J Lee María I Gaitán Seung-Kwon Ha Nicholas J Luciano Snehashis Roy Benjamin Ineichen Emily C Leibovitch Cecil C Yen Dzung L Pham Afonso C Silva Mac Johnson Steve Jacobson Pascal Sati Daniel S Reich |
| author_sort | Maxime Donadieu |
| collection | DOAJ |
| description | Remyelination is crucial to recover from inflammatory demyelination in multiple sclerosis (MS). Investigating remyelination in vivo using magnetic resonance imaging (MRI) is difficult in MS, where collecting serial short-interval scans is challenging. Using experimental autoimmune encephalomyelitis (EAE) in common marmosets, a model of MS that recapitulates focal cerebral inflammatory demyelinating lesions, we investigated whether MRI is sensitive to, and can characterize, remyelination. In six animals followed with multisequence 7 T MRI, 31 focal lesions, predicted to be demyelinated or remyelinated based on signal intensity on proton density-weighted images, were subsequently assessed with histopathology. Remyelination occurred in four of six marmosets and 45% of lesions. Radiological-pathological comparison showed that MRI had high statistical sensitivity (100%) and specificity (90%) for detecting remyelination. This study demonstrates the prevalence of spontaneous remyelination in marmoset EAE and the ability of in vivo MRI to detect it, with implications for preclinical testing of pro-remyelinating agents. |
| first_indexed | 2024-04-09T13:24:23Z |
| format | Article |
| id | doaj.art-82b3b20c13e342e28b70423fafd616cb |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-09T13:24:23Z |
| publishDate | 2023-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-82b3b20c13e342e28b70423fafd616cb2023-05-10T15:56:48ZengeLife Sciences Publications LtdeLife2050-084X2023-04-011210.7554/eLife.73786In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosisMaxime Donadieu0https://orcid.org/0009-0003-7349-1648Nathanael J Lee1María I Gaitán2Seung-Kwon Ha3Nicholas J Luciano4Snehashis Roy5Benjamin Ineichen6Emily C Leibovitch7Cecil C Yen8Dzung L Pham9Afonso C Silva10Mac Johnson11Steve Jacobson12https://orcid.org/0000-0003-3127-1287Pascal Sati13https://orcid.org/0000-0002-6763-0125Daniel S Reich14https://orcid.org/0000-0002-2628-4334Translational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesTranslational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States; Department of Neurology and Neurological Sciences, Stanford University, Palo Alto, United StatesTranslational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesTranslational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States; Department of Neurobiology, University of Pittsburgh, Pittsburgh, United StatesTranslational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesSection on Neural Function, National Institute of Mental Health, National Institutes of Health, Bethesda, United StatesTranslational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States; Department of Neuroradiology, Clinical Neuroscience Center, University Hospital Zurich, University of Zurich, Switzerland, SwitzerlandViral Immunology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesCerebral Microcirculation Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesDepartment of Radiology and Radiological Sciences, Uniformed Services University of the Health Sciences, Bethesda, United StatesDepartment of Neurobiology, University of Pittsburgh, Pittsburgh, United States; Cerebral Microcirculation Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesVertex Pharmaceuticals Incorporated, Boston, United StatesViral Immunology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesTranslational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States; Neuroimaging Program, Department of Neurology, Cedars Sinai, Los Angeles, United StatesTranslational Neuroradiology Section, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesRemyelination is crucial to recover from inflammatory demyelination in multiple sclerosis (MS). Investigating remyelination in vivo using magnetic resonance imaging (MRI) is difficult in MS, where collecting serial short-interval scans is challenging. Using experimental autoimmune encephalomyelitis (EAE) in common marmosets, a model of MS that recapitulates focal cerebral inflammatory demyelinating lesions, we investigated whether MRI is sensitive to, and can characterize, remyelination. In six animals followed with multisequence 7 T MRI, 31 focal lesions, predicted to be demyelinated or remyelinated based on signal intensity on proton density-weighted images, were subsequently assessed with histopathology. Remyelination occurred in four of six marmosets and 45% of lesions. Radiological-pathological comparison showed that MRI had high statistical sensitivity (100%) and specificity (90%) for detecting remyelination. This study demonstrates the prevalence of spontaneous remyelination in marmoset EAE and the ability of in vivo MRI to detect it, with implications for preclinical testing of pro-remyelinating agents.https://elifesciences.org/articles/73786marmosetEAEMRIremyelinationprimatehistopathology |
| spellingShingle | Maxime Donadieu Nathanael J Lee María I Gaitán Seung-Kwon Ha Nicholas J Luciano Snehashis Roy Benjamin Ineichen Emily C Leibovitch Cecil C Yen Dzung L Pham Afonso C Silva Mac Johnson Steve Jacobson Pascal Sati Daniel S Reich In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis eLife marmoset EAE MRI remyelination primate histopathology |
| title | In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis |
| title_full | In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis |
| title_fullStr | In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis |
| title_full_unstemmed | In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis |
| title_short | In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis |
| title_sort | in vivo mri is sensitive to remyelination in a nonhuman primate model of multiple sclerosis |
| topic | marmoset EAE MRI remyelination primate histopathology |
| url | https://elifesciences.org/articles/73786 |
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