Detection and persistence of environmental DNA from an invasive, terrestrial mammal
Abstract Invasive Sus scrofa, a species commonly referred to as wild pig or feral swine, is a destructive invasive species with a rapidly expanding distribution across the United States. We used artificial wallows and small waterers to determine the minimum amount of time needed for pig eDNA to accu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.3698 |
| _version_ | 1818948846180368384 |
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| author | Kelly E. Williams Kathryn P. Huyvaert Kurt C. Vercauteren Amy J. Davis Antoinette J. Piaggio |
| author_facet | Kelly E. Williams Kathryn P. Huyvaert Kurt C. Vercauteren Amy J. Davis Antoinette J. Piaggio |
| author_sort | Kelly E. Williams |
| collection | DOAJ |
| description | Abstract Invasive Sus scrofa, a species commonly referred to as wild pig or feral swine, is a destructive invasive species with a rapidly expanding distribution across the United States. We used artificial wallows and small waterers to determine the minimum amount of time needed for pig eDNA to accumulate in the water source to a detectable level. We removed water from the artificial wallows and tested eDNA detection over the course of 2 weeks to understand eDNA persistence. We show that our method is sensitive enough to detect very low quantities of eDNA shed by a terrestrial mammal that has limited interaction with water. Our experiments suggest that the number of individuals shedding into a water system can affect persistence of eDNA. Use of an eDNA detection technique can benefit management efforts by providing a sensitive method for finding even small numbers of individuals that may be elusive using other methods. |
| first_indexed | 2024-12-20T08:53:17Z |
| format | Article |
| id | doaj.art-82b451555f2d41e5aac82e6705894318 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-20T08:53:17Z |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-82b451555f2d41e5aac82e67058943182022-12-21T19:46:05ZengWileyEcology and Evolution2045-77582018-01-018168869510.1002/ece3.3698Detection and persistence of environmental DNA from an invasive, terrestrial mammalKelly E. Williams0Kathryn P. Huyvaert1Kurt C. Vercauteren2Amy J. Davis3Antoinette J. Piaggio4Wildlife Genetics Lab USDA, Wildlife Services, National Wildlife Research Center Fort Collins CO USADepartment of Fish, Wildlife, and Conservation Biology Colorado State University Fort Collins CO USAWildlife Genetics Lab USDA, Wildlife Services, National Wildlife Research Center Fort Collins CO USAWildlife Genetics Lab USDA, Wildlife Services, National Wildlife Research Center Fort Collins CO USAWildlife Genetics Lab USDA, Wildlife Services, National Wildlife Research Center Fort Collins CO USAAbstract Invasive Sus scrofa, a species commonly referred to as wild pig or feral swine, is a destructive invasive species with a rapidly expanding distribution across the United States. We used artificial wallows and small waterers to determine the minimum amount of time needed for pig eDNA to accumulate in the water source to a detectable level. We removed water from the artificial wallows and tested eDNA detection over the course of 2 weeks to understand eDNA persistence. We show that our method is sensitive enough to detect very low quantities of eDNA shed by a terrestrial mammal that has limited interaction with water. Our experiments suggest that the number of individuals shedding into a water system can affect persistence of eDNA. Use of an eDNA detection technique can benefit management efforts by providing a sensitive method for finding even small numbers of individuals that may be elusive using other methods.https://doi.org/10.1002/ece3.3698environmental DNAinvasive speciesSus scrofawild pig |
| spellingShingle | Kelly E. Williams Kathryn P. Huyvaert Kurt C. Vercauteren Amy J. Davis Antoinette J. Piaggio Detection and persistence of environmental DNA from an invasive, terrestrial mammal Ecology and Evolution environmental DNA invasive species Sus scrofa wild pig |
| title | Detection and persistence of environmental DNA from an invasive, terrestrial mammal |
| title_full | Detection and persistence of environmental DNA from an invasive, terrestrial mammal |
| title_fullStr | Detection and persistence of environmental DNA from an invasive, terrestrial mammal |
| title_full_unstemmed | Detection and persistence of environmental DNA from an invasive, terrestrial mammal |
| title_short | Detection and persistence of environmental DNA from an invasive, terrestrial mammal |
| title_sort | detection and persistence of environmental dna from an invasive terrestrial mammal |
| topic | environmental DNA invasive species Sus scrofa wild pig |
| url | https://doi.org/10.1002/ece3.3698 |
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