Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System
Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. Materials and methods Bov...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2010-02-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45795_3a8f12fe06cf5f25fcaaec8a1d00d022.pdf |
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| author | Mohsen Forouzanfar Sayyed Mortaza Hosseini Mehdi Hajian Fariba Molavi Parvaneh Abedi Mohammad Hossein Nasr Esfahani |
| author_facet | Mohsen Forouzanfar Sayyed Mortaza Hosseini Mehdi Hajian Fariba Molavi Parvaneh Abedi Mohammad Hossein Nasr Esfahani |
| author_sort | Mohsen Forouzanfar |
| collection | DOAJ |
| description | Background
This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality of bovine embryos.
Materials and methods
Bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. Their presumptive zygotes were cultured in either TCM199 or B2 culture media in conjunction with vero cells for up to nine days. The culture media were refreshed every two days and the proportion of embryos that cleaved and further developed to the morula and blastocyst (early expand and hatched) stages were recorded. Hatched blastocysts underwent differential staining in order to determine the numbers of inner cell mass (ICM) and tropho ectoderm (TE) and total cell number (TCN).
Results
Of the two groups no significant difference was seen between the proportions of the presumptive zygotes cleaved those which developed to 8-16 cells morula and reached days 7or 8 blastocyst stage or hatched. However the values for TCN and TE of the TCM199- vero embryos were significantly greater than those of B2-vero embryos. The values for ICM/ TCN and ICM/TE were significantly greater in the B2-vero group versus the TCM199-vero group.
Conclusion
Both TCM199 and B2 culture media in conjunction with vero cells were of the same efficiency when used for in vitro development of bovine presumptive zygotes. However TCM199 was superior in providing embryos with more embryo cell numbers whereas B2 medium was superior in providing embryos with greater ICM/TE and ICM/ TCN ratios. |
| first_indexed | 2024-12-14T22:56:17Z |
| format | Article |
| id | doaj.art-82b76d85e4f042309d86cb3675d69e8c |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-14T22:56:17Z |
| publishDate | 2010-02-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-82b76d85e4f042309d86cb3675d69e8c2022-12-21T22:44:34ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782010-02-013417117610.22074/ijfs.2010.4579545795Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture SystemMohsen Forouzanfar0Sayyed Mortaza Hosseini1Mehdi Hajian2Fariba Molavi3Parvaneh Abedi4Mohammad Hossein Nasr Esfahani5Faculty of Basic Science, Islamic Azad University, Marvdasht Branch, Marvdasht, IranReproduction and Development Department, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranReproduction and Development Department, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranReproduction and Development Department, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranReproduction and Development Department, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranReproduction and Development Department, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranBackground This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. Materials and methods Bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. Their presumptive zygotes were cultured in either TCM199 or B2 culture media in conjunction with vero cells for up to nine days. The culture media were refreshed every two days and the proportion of embryos that cleaved and further developed to the morula and blastocyst (early expand and hatched) stages were recorded. Hatched blastocysts underwent differential staining in order to determine the numbers of inner cell mass (ICM) and tropho ectoderm (TE) and total cell number (TCN). Results Of the two groups no significant difference was seen between the proportions of the presumptive zygotes cleaved those which developed to 8-16 cells morula and reached days 7or 8 blastocyst stage or hatched. However the values for TCN and TE of the TCM199- vero embryos were significantly greater than those of B2-vero embryos. The values for ICM/ TCN and ICM/TE were significantly greater in the B2-vero group versus the TCM199-vero group. Conclusion Both TCM199 and B2 culture media in conjunction with vero cells were of the same efficiency when used for in vitro development of bovine presumptive zygotes. However TCM199 was superior in providing embryos with more embryo cell numbers whereas B2 medium was superior in providing embryos with greater ICM/TE and ICM/ TCN ratios.http://www.ijfs.ir/article_45795_3a8f12fe06cf5f25fcaaec8a1d00d022.pdfin vitro fertilizationembryo culture techniquesco-culture |
| spellingShingle | Mohsen Forouzanfar Sayyed Mortaza Hosseini Mehdi Hajian Fariba Molavi Parvaneh Abedi Mohammad Hossein Nasr Esfahani Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System International Journal of Fertility and Sterility in vitro fertilization embryo culture techniques co-culture |
| title | Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System |
| title_full | Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System |
| title_fullStr | Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System |
| title_full_unstemmed | Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System |
| title_short | Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System |
| title_sort | differential effect of medium on the ratio of icm te of bovine embryos in a co culture system |
| topic | in vitro fertilization embryo culture techniques co-culture |
| url | http://www.ijfs.ir/article_45795_3a8f12fe06cf5f25fcaaec8a1d00d022.pdf |
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