Isolation, Molecular Identification and Evaluation of Antifungal Effect of Dominant Lactic Acid Bacteria Isolated from Wheat Bran and Rice Bran Sourdoughs
Sourdough is a proper fermented ecosystem for isolation of lactic acid bacteria (LAB) with antifungal effects. In this research, dominant LAB isolated from rice bran and wheat bran sourdoughs were identified based on molecular method. Antifungal activity of the isolates and their cell free culture f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
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Research Institute of Food Science and Technology
2017-11-01
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| Series: | Pizhūhish va Nuāvarī dar ̒Ulūm va Sanāyi̒-i Ghaz̠āyī |
| Subjects: | |
| Online Access: | http://journals.rifst.ac.ir/article_68289_45c5e662c188305ff375175c6398e9e3.pdf |
| Summary: | Sourdough is a proper fermented ecosystem for isolation of lactic acid bacteria (LAB) with antifungal effects. In this research, dominant LAB isolated from rice bran and wheat bran sourdoughs were identified based on molecular method. Antifungal activity of the isolates and their cell free culture filtrate (CCF) obtained from logarithmic and stationary phases were also investigated based on overlay and spore spot methods respectively, against <em>Aspergillus flavus</em> and <em>Aspergillus niger</em>. According to sequencing the results of PCR products, <em>Lactobacillus paraplantarum </em>in wheat bran sourdough and <em>Pediococcus pentosaceus</em> in rice bran sourdough were identified as dominant isolated LAB. The results of overlay method also confirmed the inhibitory potential of the isolates against indicator fungi in comparison to control sample. Furthermore, inhibitory effect of the LAB isolates against <em>A. flavus</em> was significantly higher than the <em>A. niger</em> (<em>P</em><0.05). The inhibitory effect of CCF obtained from each bacterium logarithmic and stationary phases against <em>A. flavus</em> were also significantly different but between inhibitory effect of <em>L. paraplantarum</em> stationary CCF and <em>P.</em> <em>pentosaceus</em> logarithmic CCF against this fungus, significant difference was not observed (<em>P</em><0.05). The inhibitory effect of CCF obtained from the isolates logarithmic and stationary phases against <em>A. niger </em>were also not significantly different (<em>P</em><0.05). |
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| ISSN: | 2252-0937 2538-2357 |