GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer Cells
Graphene oxide (GO) based nanocomposites have attracted lot of attention in the biomedical field, especially for the diagnosis and treatment of tumors. Using invitro studies, recent reports have illustrated the ability of graphene oxide for selective killing of several cancer cell lines. Coupling of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2017-03-01
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| Series: | Nano Biomedicine and Engineering |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v9i1.p96-102 |
| _version_ | 1797673383482097664 |
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| author | Venkatesha Narayanaswamy Yasrib Qurishi Chandan Srivastava |
| author_facet | Venkatesha Narayanaswamy Yasrib Qurishi Chandan Srivastava |
| author_sort | Venkatesha Narayanaswamy |
| collection | DOAJ |
| description | Graphene oxide (GO) based nanocomposites have attracted lot of attention in the biomedical field, especially for the diagnosis and treatment of tumors. Using invitro studies, recent reports have illustrated the ability of graphene oxide for selective killing of several cancer cell lines. Coupling of the anticancer property of graphene oxide with magnetic nanoparticles makes the graphene oxide-nanoparticle composite a potential material for diagnosis and treatment of cancer. In this work, GO-Fe3O4 nanoparticle composite was synthesized by the co-precipitation method. The GO-Fe3O4 nanoparticle composite was thoroughly analyzed for their potential towards killing of cancer cell lines by various assays like MTT, cell cycle analysis by flow cytometry, flow cytometric analysis of apoptosis and necrosis, apoptotic characterization of HL-60 cells using fluorescence microscopy, and flow cytometric measurement of intracellular peroxides (ROS). All these biological end-points indicated cell death by apoptosis. Here, we report that GO-Fe3O4 composites interact with HeLa and cause dose dependent cytotoxicity which robustly induced cell cycle arrest, annexin V-FITC staining and reactive oxygen species generation. |
| first_indexed | 2024-03-11T21:44:40Z |
| format | Article |
| id | doaj.art-82b9f8ad42d9448fa2d3ee9e06509d6a |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2024-03-11T21:44:40Z |
| publishDate | 2017-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-82b9f8ad42d9448fa2d3ee9e06509d6a2023-09-26T10:55:26ZengTsinghua University PressNano Biomedicine and Engineering2150-55782017-03-01919610210.5101/nbe.v9i1.p96-102GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer CellsVenkatesha Narayanaswamy0Yasrib Qurishi1Chandan Srivastava2Department of Materials Engineering, Indian Institute of Science, Bangalore, IndiaDepartment of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, IndiaDepartment of Materials Engineering, Indian Institute of Science, Bangalore, IndiaGraphene oxide (GO) based nanocomposites have attracted lot of attention in the biomedical field, especially for the diagnosis and treatment of tumors. Using invitro studies, recent reports have illustrated the ability of graphene oxide for selective killing of several cancer cell lines. Coupling of the anticancer property of graphene oxide with magnetic nanoparticles makes the graphene oxide-nanoparticle composite a potential material for diagnosis and treatment of cancer. In this work, GO-Fe3O4 nanoparticle composite was synthesized by the co-precipitation method. The GO-Fe3O4 nanoparticle composite was thoroughly analyzed for their potential towards killing of cancer cell lines by various assays like MTT, cell cycle analysis by flow cytometry, flow cytometric analysis of apoptosis and necrosis, apoptotic characterization of HL-60 cells using fluorescence microscopy, and flow cytometric measurement of intracellular peroxides (ROS). All these biological end-points indicated cell death by apoptosis. Here, we report that GO-Fe3O4 composites interact with HeLa and cause dose dependent cytotoxicity which robustly induced cell cycle arrest, annexin V-FITC staining and reactive oxygen species generation.https://www.sciopen.com/article/10.5101/nbe.v9i1.p96-102graphene oxideanticancerapoptosismttcytometry |
| spellingShingle | Venkatesha Narayanaswamy Yasrib Qurishi Chandan Srivastava GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer Cells Nano Biomedicine and Engineering graphene oxide anticancer apoptosis mtt cytometry |
| title | GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer Cells |
| title_full | GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer Cells |
| title_fullStr | GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer Cells |
| title_full_unstemmed | GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer Cells |
| title_short | GO-Fe3O4 Nanoparticle Composite for Selective Targeting of Cancer Cells |
| title_sort | go fe3o4 nanoparticle composite for selective targeting of cancer cells |
| topic | graphene oxide anticancer apoptosis mtt cytometry |
| url | https://www.sciopen.com/article/10.5101/nbe.v9i1.p96-102 |
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