Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B
Phthalates are widely distributed in our environment due to their usage in many industries, especially in plastic production, which has become an essential part of daily life. This investigation aimed to assess the potential remedial influence of lutein, a naturally occurring carotenoid, on phthalat...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/9/742 |
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| author | Dina R. S. Gad El-Karim Mohamed A. Lebda Badriyah S. Alotaibi Attalla F. El-kott Heba I. Ghamry Mustafa Shukry |
| author_facet | Dina R. S. Gad El-Karim Mohamed A. Lebda Badriyah S. Alotaibi Attalla F. El-kott Heba I. Ghamry Mustafa Shukry |
| author_sort | Dina R. S. Gad El-Karim |
| collection | DOAJ |
| description | Phthalates are widely distributed in our environment due to their usage in many industries, especially in plastic production, which has become an essential part of daily life. This investigation aimed to assess the potential remedial influence of lutein, a naturally occurring carotenoid, on phthalate-triggered damage to the liver and kidneys. When di-(2-ethylhexyl) phthalate (DEHP) was administered to male albino rats over sixty straight days at a dosage of 200 mg/kg body weight, it resulted in a significant increase in the serum activity of liver enzymes (AST, ALT, and GGT), alpha-fetoprotein, creatinine, and cystatin-C, as well as disruptions in the serum protein profile. In addition, intoxication with DEHP affected hepato-renal tissues’ redox balance. It increased the content of some proinflammatory cytokines, nuclear factor kappa B (Nf-κB), and apoptotic marker (caspase-3); likewise, DEHP-induced toxicity and decreased the level of anti-apoptotic protein (Bcl-2) in these tissues. Lutein administration at a dose level of 40 mg/kg b.w efficiently facilitated the changes in serum biochemical constituents, hepato-renal oxidative disturbance, and inflammatory, apoptotic, and histopathological alterations induced by DEHP intoxication. In conclusion, it can be presumed that lutein is protective as a natural carotenoid against DEHP toxicity. |
| first_indexed | 2024-03-10T21:53:43Z |
| format | Article |
| id | doaj.art-82be67ec010943a49f8cc4e08dfbc91c |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T21:53:43Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-82be67ec010943a49f8cc4e08dfbc91c2023-11-19T13:14:31ZengMDPI AGToxics2305-63042023-08-0111974210.3390/toxics11090742Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa BDina R. S. Gad El-Karim0Mohamed A. Lebda1Badriyah S. Alotaibi2Attalla F. El-kott3Heba I. Ghamry4Mustafa Shukry5Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, EgyptDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Biology, College of Science, King Khalid University, Abha 61421, Saudi ArabiaNutrition and Food Sciences, Department of Home Economics, Faculty of Home Economics, King Khalid University, P.O. Box 960, Abha 61421, Saudi ArabiaPhysiology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptPhthalates are widely distributed in our environment due to their usage in many industries, especially in plastic production, which has become an essential part of daily life. This investigation aimed to assess the potential remedial influence of lutein, a naturally occurring carotenoid, on phthalate-triggered damage to the liver and kidneys. When di-(2-ethylhexyl) phthalate (DEHP) was administered to male albino rats over sixty straight days at a dosage of 200 mg/kg body weight, it resulted in a significant increase in the serum activity of liver enzymes (AST, ALT, and GGT), alpha-fetoprotein, creatinine, and cystatin-C, as well as disruptions in the serum protein profile. In addition, intoxication with DEHP affected hepato-renal tissues’ redox balance. It increased the content of some proinflammatory cytokines, nuclear factor kappa B (Nf-κB), and apoptotic marker (caspase-3); likewise, DEHP-induced toxicity and decreased the level of anti-apoptotic protein (Bcl-2) in these tissues. Lutein administration at a dose level of 40 mg/kg b.w efficiently facilitated the changes in serum biochemical constituents, hepato-renal oxidative disturbance, and inflammatory, apoptotic, and histopathological alterations induced by DEHP intoxication. In conclusion, it can be presumed that lutein is protective as a natural carotenoid against DEHP toxicity.https://www.mdpi.com/2305-6304/11/9/742phthalatesluteinhepato-nephrotoxicityratsNf-κB |
| spellingShingle | Dina R. S. Gad El-Karim Mohamed A. Lebda Badriyah S. Alotaibi Attalla F. El-kott Heba I. Ghamry Mustafa Shukry Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B Toxics phthalates lutein hepato-nephrotoxicity rats Nf-κB |
| title | Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B |
| title_full | Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B |
| title_fullStr | Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B |
| title_full_unstemmed | Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B |
| title_short | Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B |
| title_sort | lutein modulates oxidative stress inflammatory and apoptotic biomarkers related to di 2 ethylhexyl phthalate dehp hepato nephrotoxicity in male rats role of nuclear factor kappa b |
| topic | phthalates lutein hepato-nephrotoxicity rats Nf-κB |
| url | https://www.mdpi.com/2305-6304/11/9/742 |
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