Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties
Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recomb...
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MDPI AG
2021-11-01
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| author | María José Diéguez Micaela López Emiliano Altieri María Fernanda Pergolesi Marisol Alicia Dabove Alba Romina Cuyeu Nadia Justus Mariana Kandus Lorena Ingala Francisco Sacco |
| author_facet | María José Diéguez Micaela López Emiliano Altieri María Fernanda Pergolesi Marisol Alicia Dabove Alba Romina Cuyeu Nadia Justus Mariana Kandus Lorena Ingala Francisco Sacco |
| author_sort | María José Diéguez |
| collection | DOAJ |
| description | Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene <i>LrBMP1</i> was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene <i>LrSV1</i>, the APR genetic system <i>LrSV2</i> + <i>LrcSV2</i> and the ASR (All Stage Resistance) gene <i>LrG6</i>. Using backcrosses, <i>LrBMP1</i> was introgressed in four commercial susceptible varieties and <i>LrSV1</i>, <i>LrSV2</i> + <i>LrcSV2</i> and <i>LrG6</i> were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system <i>LrSV2</i> + <i>LrcSV2</i> for leaf rust APR is reported. |
| first_indexed | 2024-03-10T05:42:19Z |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T05:42:19Z |
| publishDate | 2021-11-01 |
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| series | Biology |
| spelling | doaj.art-82c0993937684269a8bd0ba301b43a942023-11-22T22:28:21ZengMDPI AGBiology2079-77372021-11-011011116810.3390/biology10111168Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable VarietiesMaría José Diéguez0Micaela López1Emiliano Altieri2María Fernanda Pergolesi3Marisol Alicia Dabove4Alba Romina Cuyeu5Nadia Justus6Mariana Kandus7Lorena Ingala8Francisco Sacco9Instituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaInstituto de Genética “Ewald A. Favret”, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, ArgentinaLeaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene <i>LrBMP1</i> was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene <i>LrSV1</i>, the APR genetic system <i>LrSV2</i> + <i>LrcSV2</i> and the ASR (All Stage Resistance) gene <i>LrG6</i>. Using backcrosses, <i>LrBMP1</i> was introgressed in four commercial susceptible varieties and <i>LrSV1</i>, <i>LrSV2</i> + <i>LrcSV2</i> and <i>LrG6</i> were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system <i>LrSV2</i> + <i>LrcSV2</i> for leaf rust APR is reported.https://www.mdpi.com/2079-7737/10/11/1168wheat leaf rustdurable resistanceadult plant resistance genesmarker-assisted selectiongene introgressionfine mapping |
| spellingShingle | María José Diéguez Micaela López Emiliano Altieri María Fernanda Pergolesi Marisol Alicia Dabove Alba Romina Cuyeu Nadia Justus Mariana Kandus Lorena Ingala Francisco Sacco Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties Biology wheat leaf rust durable resistance adult plant resistance genes marker-assisted selection gene introgression fine mapping |
| title | Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties |
| title_full | Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties |
| title_fullStr | Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties |
| title_full_unstemmed | Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties |
| title_short | Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties |
| title_sort | characterization and use in wheat breeding of leaf rust resistance genes from durable varieties |
| topic | wheat leaf rust durable resistance adult plant resistance genes marker-assisted selection gene introgression fine mapping |
| url | https://www.mdpi.com/2079-7737/10/11/1168 |
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