A Comparison of Homogenization vs. Enzymatic Lysis for Microbiome Profiling in Clinical Endoscopic Biopsy Tissue Samples

A Comparison of Homogenization vs. Enzymatic Lysis for Microbiome Profiling in Clinical Endoscopic Biopsy Tissue Samples

Identification of the human microbiome has proven to be of utmost importance with the emerging role of bacteria in various physiological and pathological processes. High throughput sequencing strategies have evolved to assess the composition of the microbiome. To identify possible bias that may exis...

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Bibliographic Details
Main Authors: Chao Zhang, Prashant V. Thakkar, Sarah Ellen Powell, Prateek Sharma, Sreekar Vennelaganti, Doron Betel, Manish A. Shah
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-01-01
Series:Frontiers in Microbiology
Subjects:
gastrointestinal tract
whole genome sequencing
quantitative PCR
microbiome
clinical biopsy
metagenomics
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.03246/full

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