Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair
Abstract The effectiveness of existing tissue‐engineering cartilage (TEC) is known to be hampered by weak integration of biocompatibility, biodegradation, mechanical strength, and microenvironment supplies. The strategy of hydrogel‐based TEC holds considerable promise in circumventing these problems...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-12-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202105571 |
| _version_ | 1811197893742166016 |
|---|---|
| author | You‐Rong Chen Xin Yan Fu‐Zhen Yuan Lin Lin Shao‐Jie Wang Jing Ye Ji‐Ying Zhang Meng Yang De‐Cheng Wu Xing Wang Jia‐Kuo Yu |
| author_facet | You‐Rong Chen Xin Yan Fu‐Zhen Yuan Lin Lin Shao‐Jie Wang Jing Ye Ji‐Ying Zhang Meng Yang De‐Cheng Wu Xing Wang Jia‐Kuo Yu |
| author_sort | You‐Rong Chen |
| collection | DOAJ |
| description | Abstract The effectiveness of existing tissue‐engineering cartilage (TEC) is known to be hampered by weak integration of biocompatibility, biodegradation, mechanical strength, and microenvironment supplies. The strategy of hydrogel‐based TEC holds considerable promise in circumventing these problems. Herein, a non‐toxic, biodegradable, and mechanically optimized double‐network (DN) hydrogel consisting of polyethylene glycol (PEG) and kartogenin (KGN)‐conjugated chitosan (CHI) is constructed using a simple soaking strategy. This PEG‐CHI‐KGN DN hydrogel possesses favorable architectures, suitable mechanics, remarkable cellular affinity, and sustained KGN release, which can facilitate the cartilage‐specific genes expression and extracellular matrix secretion of peripheral blood‐derived mesenchymal stem cells (PB‐MSCs). Notably, after tracing the transplanted cells by detecting the rabbit sex‐determining region Y‐linked gene sequence, the allogeneic PB‐MSCs are found to survive for even 3 months in the regenerated cartilage. Here, the long‐term release of KGN is able to efficiently and persistently activate multiple genes and signaling pathways to promote the chondrogenesis, chondrocyte differentiation, and survival of PB‐MSCs. Thus, the regenerated tissues exhibit well‐matched histomorphology and biomechanical performance such as native cartilage. Consequently, it is believed this innovative work can expand the choice for developing the next generation of orthopedic implants in the loadbearing region of a living body. |
| first_indexed | 2024-04-12T01:21:47Z |
| format | Article |
| id | doaj.art-82c1188286a74913a181e8a7b53f161f |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-12T01:21:47Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-82c1188286a74913a181e8a7b53f161f2022-12-22T03:53:46ZengWileyAdvanced Science2198-38442022-12-01935n/an/a10.1002/advs.202105571Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage RepairYou‐Rong Chen0Xin Yan1Fu‐Zhen Yuan2Lin Lin3Shao‐Jie Wang4Jing Ye5Ji‐Ying Zhang6Meng Yang7De‐Cheng Wu8Xing Wang9Jia‐Kuo Yu10Department of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaDepartment of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaDepartment of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaDepartment of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaDepartment of Joint Surgery and Sports Medicine, Zhongshan Hospital Xiamen University Xiamen 361000 ChinaDepartment of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaDepartment of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaDepartment of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaDepartment of Biomedical Engineering Southern University of Science and Technology Shenzhen 518055 ChinaBeijing National Laboratory for Molecular Sciences State Key Laboratory of Polymer Physics and Chemistry Institute of Chemistry Chinese Academy of Sciences Beijing 100190 ChinaDepartment of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital Beijing 100191 ChinaAbstract The effectiveness of existing tissue‐engineering cartilage (TEC) is known to be hampered by weak integration of biocompatibility, biodegradation, mechanical strength, and microenvironment supplies. The strategy of hydrogel‐based TEC holds considerable promise in circumventing these problems. Herein, a non‐toxic, biodegradable, and mechanically optimized double‐network (DN) hydrogel consisting of polyethylene glycol (PEG) and kartogenin (KGN)‐conjugated chitosan (CHI) is constructed using a simple soaking strategy. This PEG‐CHI‐KGN DN hydrogel possesses favorable architectures, suitable mechanics, remarkable cellular affinity, and sustained KGN release, which can facilitate the cartilage‐specific genes expression and extracellular matrix secretion of peripheral blood‐derived mesenchymal stem cells (PB‐MSCs). Notably, after tracing the transplanted cells by detecting the rabbit sex‐determining region Y‐linked gene sequence, the allogeneic PB‐MSCs are found to survive for even 3 months in the regenerated cartilage. Here, the long‐term release of KGN is able to efficiently and persistently activate multiple genes and signaling pathways to promote the chondrogenesis, chondrocyte differentiation, and survival of PB‐MSCs. Thus, the regenerated tissues exhibit well‐matched histomorphology and biomechanical performance such as native cartilage. Consequently, it is believed this innovative work can expand the choice for developing the next generation of orthopedic implants in the loadbearing region of a living body.https://doi.org/10.1002/advs.202105571cartilage regenerationcell tracingdouble‐network hydrogelskartogeninmesenchymal stem cells |
| spellingShingle | You‐Rong Chen Xin Yan Fu‐Zhen Yuan Lin Lin Shao‐Jie Wang Jing Ye Ji‐Ying Zhang Meng Yang De‐Cheng Wu Xing Wang Jia‐Kuo Yu Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair Advanced Science cartilage regeneration cell tracing double‐network hydrogels kartogenin mesenchymal stem cells |
| title | Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair |
| title_full | Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair |
| title_fullStr | Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair |
| title_full_unstemmed | Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair |
| title_short | Kartogenin‐Conjugated Double‐Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair |
| title_sort | kartogenin conjugated double network hydrogel combined with stem cell transplantation and tracing for cartilage repair |
| topic | cartilage regeneration cell tracing double‐network hydrogels kartogenin mesenchymal stem cells |
| url | https://doi.org/10.1002/advs.202105571 |
| work_keys_str_mv | AT yourongchen kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT xinyan kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT fuzhenyuan kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT linlin kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT shaojiewang kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT jingye kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT jiyingzhang kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT mengyang kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT dechengwu kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT xingwang kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair AT jiakuoyu kartogeninconjugateddoublenetworkhydrogelcombinedwithstemcelltransplantationandtracingforcartilagerepair |