Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean
Abstract To address the demand for food by a rapidly growing human population, agricultural scientists have carried out both plant breeding and genetic engineering research. Previously, we reported that the constitutive expression of a pea apyrase (Nucleoside triphosphate, diphosphohydrolase) gene,...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14821-7 |
| _version_ | 1811328333513752576 |
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| author | Tanya Sabharwal Zhongjin Lu Robert D. Slocum Seongjoon Kang Huan Wang Han-Wei Jiang Roopadarshini Veerappa Dwight Romanovicz Ji Chul Nam Simon Birk Greg Clark Stanley J. Roux |
| author_facet | Tanya Sabharwal Zhongjin Lu Robert D. Slocum Seongjoon Kang Huan Wang Han-Wei Jiang Roopadarshini Veerappa Dwight Romanovicz Ji Chul Nam Simon Birk Greg Clark Stanley J. Roux |
| author_sort | Tanya Sabharwal |
| collection | DOAJ |
| description | Abstract To address the demand for food by a rapidly growing human population, agricultural scientists have carried out both plant breeding and genetic engineering research. Previously, we reported that the constitutive expression of a pea apyrase (Nucleoside triphosphate, diphosphohydrolase) gene, psNTP9, under the control of the CaMV35S promoter, resulted in soybean plants with an expanded root system architecture, enhanced drought resistance and increased seed yield when they are grown in greenhouses under controlled conditions. Here, we report that psNTP9-expressing soybean lines also show significantly enhanced seed yields when grown in multiple different field conditions at multiple field sites, including when the gene is introgressed into elite germplasm. The transgenic lines have higher leaf chlorophyll and soluble protein contents and decreased stomatal density and cuticle permeability, traits that increase water use efficiency and likely contribute to the increased seed yields of field-grown plants. These altered properties are explained, in part, by genome-wide gene expression changes induced by the transgene. |
| first_indexed | 2024-04-13T15:23:26Z |
| format | Article |
| id | doaj.art-82c258d7cacd4f20b3dc5f2f8c02b6d4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T15:23:26Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-82c258d7cacd4f20b3dc5f2f8c02b6d42022-12-22T02:41:34ZengNature PortfolioScientific Reports2045-23222022-06-0112112010.1038/s41598-022-14821-7Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybeanTanya Sabharwal0Zhongjin Lu1Robert D. Slocum2Seongjoon Kang3Huan Wang4Han-Wei Jiang5Roopadarshini Veerappa6Dwight Romanovicz7Ji Chul Nam8Simon Birk9Greg Clark10Stanley J. Roux11Department of Molecular Biosciences, The University of Texas at AustinTexas Crop Science, Inc.Program in Biological Sciences, Goucher CollegeDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinDepartment of Molecular Biosciences, The University of Texas at AustinAbstract To address the demand for food by a rapidly growing human population, agricultural scientists have carried out both plant breeding and genetic engineering research. Previously, we reported that the constitutive expression of a pea apyrase (Nucleoside triphosphate, diphosphohydrolase) gene, psNTP9, under the control of the CaMV35S promoter, resulted in soybean plants with an expanded root system architecture, enhanced drought resistance and increased seed yield when they are grown in greenhouses under controlled conditions. Here, we report that psNTP9-expressing soybean lines also show significantly enhanced seed yields when grown in multiple different field conditions at multiple field sites, including when the gene is introgressed into elite germplasm. The transgenic lines have higher leaf chlorophyll and soluble protein contents and decreased stomatal density and cuticle permeability, traits that increase water use efficiency and likely contribute to the increased seed yields of field-grown plants. These altered properties are explained, in part, by genome-wide gene expression changes induced by the transgene.https://doi.org/10.1038/s41598-022-14821-7 |
| spellingShingle | Tanya Sabharwal Zhongjin Lu Robert D. Slocum Seongjoon Kang Huan Wang Han-Wei Jiang Roopadarshini Veerappa Dwight Romanovicz Ji Chul Nam Simon Birk Greg Clark Stanley J. Roux Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean Scientific Reports |
| title | Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean |
| title_full | Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean |
| title_fullStr | Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean |
| title_full_unstemmed | Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean |
| title_short | Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean |
| title_sort | constitutive expression of a pea apyrase psntp9 increases seed yield in field grown soybean |
| url | https://doi.org/10.1038/s41598-022-14821-7 |
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