A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins
Plasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template-assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/9/1400 |
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| author | Francesco Arcadio Mimimorena Seggio Domenico Del Prete Gionatan Buonanno João Mendes Luís C. C. Coelho Pedro A. S. Jorge Luigi Zeni Alessandra Maria Bossi Nunzio Cennamo |
| author_facet | Francesco Arcadio Mimimorena Seggio Domenico Del Prete Gionatan Buonanno João Mendes Luís C. C. Coelho Pedro A. S. Jorge Luigi Zeni Alessandra Maria Bossi Nunzio Cennamo |
| author_sort | Francesco Arcadio |
| collection | DOAJ |
| description | Plasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template-assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting soft nanoMIPs, which are known to deform upon analyte binding. This work presents the development of a surface plasmon resonance (SPR) sensor in silica light-diffusing fibers (LDFs) functionalized with a specific nanoMIP receptor, entailed for the recognition of the protein human serum transferrin (HTR). Despite their great versatility, to date only SPR-LFDs functionalized with antibodies have been reported. Here, the innovative combination of an SPR-LFD platform and nanoMIPs led to the development of a sensor with an ultra-low limit of detection (LOD), equal to about 4 fM, and selective for its target analyte HTR. It is worth noting that the SPR-LDF-nanoMIP sensor was mounted within a specially designed 3D-printed holder yielding a measurement cell suitable for a rapid and reliable setup, and easy for the scaling up of the measurements. Moreover, the fabrication process to realize the SPR platform is minimal, requiring only a metal deposition step. |
| first_indexed | 2024-03-10T03:52:46Z |
| format | Article |
| id | doaj.art-82ca50b56e454fa788b17bd29fb640d3 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:52:46Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-82ca50b56e454fa788b17bd29fb640d32023-11-23T08:53:37ZengMDPI AGNanomaterials2079-49912022-04-01129140010.3390/nano12091400A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of ProteinsFrancesco Arcadio0Mimimorena Seggio1Domenico Del Prete2Gionatan Buonanno3João Mendes4Luís C. C. Coelho5Pedro A. S. Jorge6Luigi Zeni7Alessandra Maria Bossi8Nunzio Cennamo9Department of Engineering, University of Campania Luigi Vanvitelli, Via Roma 29, 81031 Aversa, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, ItalyDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma 29, 81031 Aversa, ItalyDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma 29, 81031 Aversa, ItalyINESC TEC–Institute for Systems and Computer Engineering, Technology and Science, Faculty of Sciences, University of Porto, 4169-007 Porto, PortugalINESC TEC–Institute for Systems and Computer Engineering, Technology and Science, Faculty of Sciences, University of Porto, 4169-007 Porto, PortugalINESC TEC–Institute for Systems and Computer Engineering, Technology and Science, Faculty of Sciences, University of Porto, 4169-007 Porto, PortugalDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma 29, 81031 Aversa, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, ItalyDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma 29, 81031 Aversa, ItalyPlasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template-assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting soft nanoMIPs, which are known to deform upon analyte binding. This work presents the development of a surface plasmon resonance (SPR) sensor in silica light-diffusing fibers (LDFs) functionalized with a specific nanoMIP receptor, entailed for the recognition of the protein human serum transferrin (HTR). Despite their great versatility, to date only SPR-LFDs functionalized with antibodies have been reported. Here, the innovative combination of an SPR-LFD platform and nanoMIPs led to the development of a sensor with an ultra-low limit of detection (LOD), equal to about 4 fM, and selective for its target analyte HTR. It is worth noting that the SPR-LDF-nanoMIP sensor was mounted within a specially designed 3D-printed holder yielding a measurement cell suitable for a rapid and reliable setup, and easy for the scaling up of the measurements. Moreover, the fabrication process to realize the SPR platform is minimal, requiring only a metal deposition step.https://www.mdpi.com/2079-4991/12/9/1400light-diffusing fibers (LDFs)surface plasmon resonance (SPR)molecularly imprinted polymers (MIPs)molecularly imprinted nanoparticles (nanoMIPs)biosensorsplasmonic optical fiber biosensors |
| spellingShingle | Francesco Arcadio Mimimorena Seggio Domenico Del Prete Gionatan Buonanno João Mendes Luís C. C. Coelho Pedro A. S. Jorge Luigi Zeni Alessandra Maria Bossi Nunzio Cennamo A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins Nanomaterials light-diffusing fibers (LDFs) surface plasmon resonance (SPR) molecularly imprinted polymers (MIPs) molecularly imprinted nanoparticles (nanoMIPs) biosensors plasmonic optical fiber biosensors |
| title | A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins |
| title_full | A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins |
| title_fullStr | A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins |
| title_full_unstemmed | A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins |
| title_short | A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins |
| title_sort | plasmonic biosensor based on light diffusing fibers functionalized with molecularly imprinted nanoparticles for ultralow sensing of proteins |
| topic | light-diffusing fibers (LDFs) surface plasmon resonance (SPR) molecularly imprinted polymers (MIPs) molecularly imprinted nanoparticles (nanoMIPs) biosensors plasmonic optical fiber biosensors |
| url | https://www.mdpi.com/2079-4991/12/9/1400 |
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