Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-Ethanol
The objectives of this study were to investigate the feasibility of encapsulating yeast cells using gellan gum by an emulsification method and to evaluate the fermentation efficiency and the reusability of the micro-bioreactors produced. It was found that yeast cells could be successfully encapsulat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-10-01
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| Series: | Pharmaceutics |
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| Online Access: | http://www.mdpi.com/1999-4923/3/4/731/ |
| _version_ | 1811187340121473024 |
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| author | Sook Mun Tan Paul Wan Sia Heng Lai Wah Chan |
| author_facet | Sook Mun Tan Paul Wan Sia Heng Lai Wah Chan |
| author_sort | Sook Mun Tan |
| collection | DOAJ |
| description | The objectives of this study were to investigate the feasibility of encapsulating yeast cells using gellan gum by an emulsification method and to evaluate the fermentation efficiency and the reusability of the micro-bioreactors produced. It was found that yeast cells could be successfully encapsulated to form relatively spherical micro-bioreactors with high specific surface area for mass transfer. Cell viability was found to be reduced by one log reduction after the emulsification process. The ethanol yield of the micro-bioreactors was comparable to that of free yeast in the first fermentation cycle. The micro-bioreactors remained intact and could be re-used up to 10 cycles of fermentation. Despite cell breakthrough, relatively high ethanol yields were obtained, indicating that the micro-bioreactors also functioned as regenerative reservoirs of yeast. |
| first_indexed | 2024-04-11T14:01:35Z |
| format | Article |
| id | doaj.art-82cda150cad34ee183629a81159d0fce |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T14:01:35Z |
| publishDate | 2011-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-82cda150cad34ee183629a81159d0fce2022-12-22T04:20:07ZengMDPI AGPharmaceutics1999-49232011-10-013473174410.3390/pharmaceutics3040731Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-EthanolSook Mun TanPaul Wan Sia HengLai Wah ChanThe objectives of this study were to investigate the feasibility of encapsulating yeast cells using gellan gum by an emulsification method and to evaluate the fermentation efficiency and the reusability of the micro-bioreactors produced. It was found that yeast cells could be successfully encapsulated to form relatively spherical micro-bioreactors with high specific surface area for mass transfer. Cell viability was found to be reduced by one log reduction after the emulsification process. The ethanol yield of the micro-bioreactors was comparable to that of free yeast in the first fermentation cycle. The micro-bioreactors remained intact and could be re-used up to 10 cycles of fermentation. Despite cell breakthrough, relatively high ethanol yields were obtained, indicating that the micro-bioreactors also functioned as regenerative reservoirs of yeast.http://www.mdpi.com/1999-4923/3/4/731/microencapsulationwater-in-oil emulsiongellan gummicro-bioreactorsbioethanol |
| spellingShingle | Sook Mun Tan Paul Wan Sia Heng Lai Wah Chan Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-Ethanol Pharmaceutics microencapsulation water-in-oil emulsion gellan gum micro-bioreactors bioethanol |
| title | Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-Ethanol |
| title_full | Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-Ethanol |
| title_fullStr | Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-Ethanol |
| title_full_unstemmed | Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-Ethanol |
| title_short | Development of Re-Usable Yeast-Gellan Gum Micro-Bioreactors for Potential Application in Continuous Fermentation to Produce Bio-Ethanol |
| title_sort | development of re usable yeast gellan gum micro bioreactors for potential application in continuous fermentation to produce bio ethanol |
| topic | microencapsulation water-in-oil emulsion gellan gum micro-bioreactors bioethanol |
| url | http://www.mdpi.com/1999-4923/3/4/731/ |
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