Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice
Background and Aim: Hyperglycemia associated with hyper- or hypo-insulinemia is a hallmark of type 2 diabetes mellitus, which is firmly linked to decreased male infertility. Recently, bee venom (BV) has shown potential health prosperities, including antidiabetic; however, no study focuses on the eff...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-09-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.15/September-2022/30.pdf |
| _version_ | 1811206721841922048 |
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| author | Sattar J. J. AL-Shaeli Talal Jabal Hussen Ali M. Ethaeb |
| author_facet | Sattar J. J. AL-Shaeli Talal Jabal Hussen Ali M. Ethaeb |
| author_sort | Sattar J. J. AL-Shaeli |
| collection | DOAJ |
| description | Background and Aim: Hyperglycemia associated with hyper- or hypo-insulinemia is a hallmark of type 2 diabetes mellitus, which is firmly linked to decreased male infertility. Recently, bee venom (BV) has shown potential health prosperities, including antidiabetic; however, no study focuses on the effect of BV on male fertility in diabetic conditions. This study aimed to detect the effect of BV on histological and hormonal alteration of the testis in diabetic mice.
Materials and Methods: Twenty adult male mice were selected and assigned to four groups: Control, diabetic (150 mg/kg alloxan), BV1 (diabetic + 0.5 mg/kg BV), and BV2 (diabetic + 1 mg/kg BV). After 35 days, the serum levels of glucose, insulin, testosterone, follicular-stimulating hormone, luteinizing hormone, and prolactin were estimated. The histological structure of the testes was also evaluated.
Results: Alloxan-induced hyperglycemia and decreased insulin concentrations were reversed significantly by BV. Furthermore, diabetic mice exhibited various alterations in fertility hormones, while these disturbances were improved considerably to normal concentrations by BV. Similarly, alloxan-induced changes in sperm and testis histological parameters such as motility, viability, abnormality, sperm count, the number and diameter of seminiferous tubules, and the number of Leydig and Sertoli cells were significantly ameliorated to the normal condition by BV. Changes in the number, size, and shape of seminiferous tubules, the number of Leydig and Sertoli cells, and initial degeneration and vacuolization in interstitial cells and spermatogonia and spermatocyte were seen in diabetic mice. All these changes were shifted almost to normal structure by BV.
Conclusion: The BV could be used as an alternative therapeutic agent that manages the markers related to diabetic conditions concomitant with the improved histological structure of the testes and hormone production to accelerate male fertility. |
| first_indexed | 2024-04-12T03:52:58Z |
| format | Article |
| id | doaj.art-82d0917c972c468a97b34794fd62c7ba |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-12T03:52:58Z |
| publishDate | 2022-09-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-82d0917c972c468a97b34794fd62c7ba2022-12-22T03:48:56ZengVeterinary WorldVeterinary World0972-89882231-09162022-09-011592357236410.14202/vetworld.2022.2357-2364Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic miceSattar J. J. AL-Shaeli0https://orcid.org/0000-0003-2669-0073Talal Jabal Hussen1https://orcid.org/0000-0003-0209-2722Ali M. Ethaeb2https://orcid.org/0000-0003-2458-8171Department of Medical Basic Sciences, College of Dentistry, Wasit University, Wasit, Iraq.Department of Medical Basic Sciences, College of Dentistry, Wasit University, Wasit, Iraq.Department of Anatomy and Histology, College of Veterinary Medicine, Wasit University, Wasit, Iraq.Background and Aim: Hyperglycemia associated with hyper- or hypo-insulinemia is a hallmark of type 2 diabetes mellitus, which is firmly linked to decreased male infertility. Recently, bee venom (BV) has shown potential health prosperities, including antidiabetic; however, no study focuses on the effect of BV on male fertility in diabetic conditions. This study aimed to detect the effect of BV on histological and hormonal alteration of the testis in diabetic mice. Materials and Methods: Twenty adult male mice were selected and assigned to four groups: Control, diabetic (150 mg/kg alloxan), BV1 (diabetic + 0.5 mg/kg BV), and BV2 (diabetic + 1 mg/kg BV). After 35 days, the serum levels of glucose, insulin, testosterone, follicular-stimulating hormone, luteinizing hormone, and prolactin were estimated. The histological structure of the testes was also evaluated. Results: Alloxan-induced hyperglycemia and decreased insulin concentrations were reversed significantly by BV. Furthermore, diabetic mice exhibited various alterations in fertility hormones, while these disturbances were improved considerably to normal concentrations by BV. Similarly, alloxan-induced changes in sperm and testis histological parameters such as motility, viability, abnormality, sperm count, the number and diameter of seminiferous tubules, and the number of Leydig and Sertoli cells were significantly ameliorated to the normal condition by BV. Changes in the number, size, and shape of seminiferous tubules, the number of Leydig and Sertoli cells, and initial degeneration and vacuolization in interstitial cells and spermatogonia and spermatocyte were seen in diabetic mice. All these changes were shifted almost to normal structure by BV. Conclusion: The BV could be used as an alternative therapeutic agent that manages the markers related to diabetic conditions concomitant with the improved histological structure of the testes and hormone production to accelerate male fertility.http://www.veterinaryworld.org/Vol.15/September-2022/30.pdfdiabetic micehyperglycemiasertoli cellsspermatogonia |
| spellingShingle | Sattar J. J. AL-Shaeli Talal Jabal Hussen Ali M. Ethaeb Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice Veterinary World diabetic mice hyperglycemia sertoli cells spermatogonia |
| title | Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice |
| title_full | Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice |
| title_fullStr | Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice |
| title_full_unstemmed | Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice |
| title_short | Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice |
| title_sort | effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice |
| topic | diabetic mice hyperglycemia sertoli cells spermatogonia |
| url | http://www.veterinaryworld.org/Vol.15/September-2022/30.pdf |
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