Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study
Background: Collagen is a significant structural protein, the integrity of which is essential to be maintained for proper homeostasis. Oral submucous fibrosis (OSMF), being a collagen metabolic disorder, may be subject to changes in amino acid profiling. Aim: The present study was attempted to...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2014-12-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/5290/10201_CE(RA1)_F(T)_PF1(AJAK)_PFA(AK).pdf |
Summary: | Background: Collagen is a significant structural protein, the
integrity of which is essential to be maintained for proper
homeostasis. Oral submucous fibrosis (OSMF), being a collagen
metabolic disorder, may be subject to changes in amino acid
profiling.
Aim: The present study was attempted to evaluate the amino acid
profile to assess its feasibility as a biological marker in OSMF.
Materials and Methods: The study group comprised of 13
patents with OSMF and the normal group comprised of 13
normal patients without associated habits or systemic disorders.
Venous blood was collected from the antecubital vein, plasma
was separated and the plasma was then subjected to high profile
liquid chromatographic analysis.
Results: The assay levels of threonine, alanine and tyrosine did
not yield any significant results. The decreased assay levels of
valine, Isoleucine and the increased assay level of methionine
and glycine observed in group II yielded significant results in
correlation with the control group. The decreased assay level
seen in phenylalanine in group II and III in correlation with group
IV is statistically significant.
Conclusion: A few amino acids have been identified which can
be used as biological markers for the severity of the disease such
as valine, methionine and phenyl alanine. Large scale studies are
required to elucidate the potential of these biological markers. |
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ISSN: | 2249-782X 0973-709X |