Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study
Background: Collagen is a significant structural protein, the integrity of which is essential to be maintained for proper homeostasis. Oral submucous fibrosis (OSMF), being a collagen metabolic disorder, may be subject to changes in amino acid profiling. Aim: The present study was attempted to...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/5290/10201_CE(RA1)_F(T)_PF1(AJAK)_PFA(AK).pdf |
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| author | Richa Goel Gheena S. Thiruvengadam Chandrasekhar Pratibha Ramani Herald J. Sherlin Anuja Natesan Priya Premkumar |
| author_facet | Richa Goel Gheena S. Thiruvengadam Chandrasekhar Pratibha Ramani Herald J. Sherlin Anuja Natesan Priya Premkumar |
| author_sort | Richa Goel |
| collection | DOAJ |
| description | Background: Collagen is a significant structural protein, the
integrity of which is essential to be maintained for proper
homeostasis. Oral submucous fibrosis (OSMF), being a collagen
metabolic disorder, may be subject to changes in amino acid
profiling.
Aim: The present study was attempted to evaluate the amino acid
profile to assess its feasibility as a biological marker in OSMF.
Materials and Methods: The study group comprised of 13
patents with OSMF and the normal group comprised of 13
normal patients without associated habits or systemic disorders.
Venous blood was collected from the antecubital vein, plasma
was separated and the plasma was then subjected to high profile
liquid chromatographic analysis.
Results: The assay levels of threonine, alanine and tyrosine did
not yield any significant results. The decreased assay levels of
valine, Isoleucine and the increased assay level of methionine
and glycine observed in group II yielded significant results in
correlation with the control group. The decreased assay level
seen in phenylalanine in group II and III in correlation with group
IV is statistically significant.
Conclusion: A few amino acids have been identified which can
be used as biological markers for the severity of the disease such
as valine, methionine and phenyl alanine. Large scale studies are
required to elucidate the potential of these biological markers. |
| first_indexed | 2024-04-12T20:29:22Z |
| format | Article |
| id | doaj.art-82d19a4fc51741389651b27381dde080 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-12T20:29:22Z |
| publishDate | 2014-12-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-82d19a4fc51741389651b27381dde0802022-12-22T03:17:46ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-12-01812ZC44ZC4810.7860/JCDR/2014/10201.5290Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) StudyRicha Goel0Gheena S.1Thiruvengadam Chandrasekhar2Pratibha Ramani3Herald J. Sherlin4Anuja Natesan5Priya Premkumar6Reader, Department of Oral Pathology, Rayat and Bahra Dental College, Sahauran, Mohali, India. Reader, Department of Oral Pathology, Saveetha Dental College and Hospitals, Chennai, India.Professor and Head, Department of Oral Pathology, Sathyabhama Dental College, Sathyabhama University, Chennai, India. Professor and HOD, Department of Oral Pathology, Saveetha Dental College, Saveetha University, Chennai, India.Reader, Department of Oral Pathology, Saveetha Dental College, Saveetha University, Chennai, India.Professor, Department of Oral Pathology, Saveetha Dental College, Saveetha University, Chennai, India. Professor, Department of Oral Pathology, Saveetha Dental College, Saveetha University, Chennai, India.Background: Collagen is a significant structural protein, the integrity of which is essential to be maintained for proper homeostasis. Oral submucous fibrosis (OSMF), being a collagen metabolic disorder, may be subject to changes in amino acid profiling. Aim: The present study was attempted to evaluate the amino acid profile to assess its feasibility as a biological marker in OSMF. Materials and Methods: The study group comprised of 13 patents with OSMF and the normal group comprised of 13 normal patients without associated habits or systemic disorders. Venous blood was collected from the antecubital vein, plasma was separated and the plasma was then subjected to high profile liquid chromatographic analysis. Results: The assay levels of threonine, alanine and tyrosine did not yield any significant results. The decreased assay levels of valine, Isoleucine and the increased assay level of methionine and glycine observed in group II yielded significant results in correlation with the control group. The decreased assay level seen in phenylalanine in group II and III in correlation with group IV is statistically significant. Conclusion: A few amino acids have been identified which can be used as biological markers for the severity of the disease such as valine, methionine and phenyl alanine. Large scale studies are required to elucidate the potential of these biological markers.https://jcdr.net/articles/PDF/5290/10201_CE(RA1)_F(T)_PF1(AJAK)_PFA(AK).pdfamino acidscollagenosmfplasma |
| spellingShingle | Richa Goel Gheena S. Thiruvengadam Chandrasekhar Pratibha Ramani Herald J. Sherlin Anuja Natesan Priya Premkumar Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study Journal of Clinical and Diagnostic Research amino acids collagen osmf plasma |
| title | Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study |
| title_full | Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study |
| title_fullStr | Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study |
| title_full_unstemmed | Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study |
| title_short | Amino Acid Profile in Oral Submucous Fibrosis: A High Performance Liquid Chromatography (HPLC) Study |
| title_sort | amino acid profile in oral submucous fibrosis a high performance liquid chromatography hplc study |
| topic | amino acids collagen osmf plasma |
| url | https://jcdr.net/articles/PDF/5290/10201_CE(RA1)_F(T)_PF1(AJAK)_PFA(AK).pdf |
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