ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSION

Introduction: Burn, depending on the degree of severity and depth, induces significant pathophysiological response of the body. Our study is the prospective study for assessment of T lymphocyte immunological changes in patients with burns, with different degrees of %TBSA and depth of burns....

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Main Authors: Selma Arslanagic, Jasenko Karamehic
Format: Article
Language:English
Published: Association of medical doctors Sanamed Novi Pazar 2020-12-01
Series:Sanamed
Subjects:
Online Access:http://www.sanamed.rs/OJS/index.php/Sanamed/article/view/462/242
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author Selma Arslanagic
Jasenko Karamehic
author_facet Selma Arslanagic
Jasenko Karamehic
author_sort Selma Arslanagic
collection DOAJ
description Introduction: Burn, depending on the degree of severity and depth, induces significant pathophysiological response of the body. Our study is the prospective study for assessment of T lymphocyte immunological changes in patients with burns, with different degrees of %TBSA and depth of burns. Research objectives: Objectives of this study were to assess %CD3+Ly, % CD4+Ly, %CD8+Ly, %CD3+HLA-DR+Ly, %CD4+Ly /CD8+Ly), of burned body with different %TBSA degrees, different depth burns and to establish predictive value of of immune suppression these parameters. Patients and methods: According to %TBSA, patients were classified into three groups: mild burns with TBSA% <15% (30 patients), group of medium burns with%TBSA from 15% -25% (30 patients) and group with%TBSA> 25% to 40% (30 patients). According to the depth of burns, patients were classified into two groups, partial-thickness burns, (39 patients), and full-thickness burns (51 patients). We followed laboratory parameters : % CD3+Ly , % CD3+ CD4+Ly, % CD3+CD8+Ly, % CD3+HLA-DR+Ly, CD4 / CD8 (%) lymphocytes (on day 7th and on day 14th). Results: Percentage of CD3+ lymphocytes was significantly lower in severe burns compared to the moderate heavy burns andsignificantly lower compared to the mild burns . Percentage of CD3+CD4+ lymphocytes was significantly lower in severe burns compared to moderate heavy burns and in relation to mild burns (results on day 14th ); also are lower in moderate severe burn compared to mild burns. On day 14th, the% CD4 / CD8 ratio was not significantly lower in the severe burns versus the moderate burns. On day 14th, the % CD4 / CD8 ratio wassignificantly lower in severe burns compared to mild burns; significantly lower in moderateburns compared to mild burns. % CD3+HLA-DR + cells was significantly lower in severe burn and moderately severe burns compared to the mild burns on day 7th, and also on day 14th . Full- thickness burns have significantly lower %CD3+lymphocytes, %CD3+CD4+ lymphocytes, %CD3+HLA-DR+ lymphocytes, ratio of % CD4/CD8 lymphocytes compared to partial-thickness burns . Conclusions: Peripheral blood T lymphocytes are one of the key indicators of immunosuppression of patients with burns of different % TBSA and different degrees of burn depth. Larger %TBSA and full- thickness burns injected stronger systemic immunosuppresion, compared to smaller %TBSA and partial-thickness burns.
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spelling doaj.art-82d19e8110d14202baa1fd7f3cbb60682024-03-02T09:38:35ZengAssociation of medical doctors Sanamed Novi PazarSanamed1452-662X2217-81712020-12-0115325526410.24125/sanamed.v15i3.462ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSIONSelma Arslanagic0Jasenko Karamehic1Clinic for Reconstructive and plastic surgery, University Medical Center Sarajevo, Sarajevo, Bosnia and HerzegovinaInstitute of Immunology, University Medical Center Sarajevo, Sarajevo, Bosnia and HerzegovinaIntroduction: Burn, depending on the degree of severity and depth, induces significant pathophysiological response of the body. Our study is the prospective study for assessment of T lymphocyte immunological changes in patients with burns, with different degrees of %TBSA and depth of burns. Research objectives: Objectives of this study were to assess %CD3+Ly, % CD4+Ly, %CD8+Ly, %CD3+HLA-DR+Ly, %CD4+Ly /CD8+Ly), of burned body with different %TBSA degrees, different depth burns and to establish predictive value of of immune suppression these parameters. Patients and methods: According to %TBSA, patients were classified into three groups: mild burns with TBSA% <15% (30 patients), group of medium burns with%TBSA from 15% -25% (30 patients) and group with%TBSA> 25% to 40% (30 patients). According to the depth of burns, patients were classified into two groups, partial-thickness burns, (39 patients), and full-thickness burns (51 patients). We followed laboratory parameters : % CD3+Ly , % CD3+ CD4+Ly, % CD3+CD8+Ly, % CD3+HLA-DR+Ly, CD4 / CD8 (%) lymphocytes (on day 7th and on day 14th). Results: Percentage of CD3+ lymphocytes was significantly lower in severe burns compared to the moderate heavy burns andsignificantly lower compared to the mild burns . Percentage of CD3+CD4+ lymphocytes was significantly lower in severe burns compared to moderate heavy burns and in relation to mild burns (results on day 14th ); also are lower in moderate severe burn compared to mild burns. On day 14th, the% CD4 / CD8 ratio was not significantly lower in the severe burns versus the moderate burns. On day 14th, the % CD4 / CD8 ratio wassignificantly lower in severe burns compared to mild burns; significantly lower in moderateburns compared to mild burns. % CD3+HLA-DR + cells was significantly lower in severe burn and moderately severe burns compared to the mild burns on day 7th, and also on day 14th . Full- thickness burns have significantly lower %CD3+lymphocytes, %CD3+CD4+ lymphocytes, %CD3+HLA-DR+ lymphocytes, ratio of % CD4/CD8 lymphocytes compared to partial-thickness burns . Conclusions: Peripheral blood T lymphocytes are one of the key indicators of immunosuppression of patients with burns of different % TBSA and different degrees of burn depth. Larger %TBSA and full- thickness burns injected stronger systemic immunosuppresion, compared to smaller %TBSA and partial-thickness burns.http://www.sanamed.rs/OJS/index.php/Sanamed/article/view/462/242burn%tbsat lymphocytesthickness of burns% cd3+ly% cd3+ cd4+ly% cd3+cd8+ly% cd3+hla-dr+lyratio % cd4 / cd8 lymphocytes
spellingShingle Selma Arslanagic
Jasenko Karamehic
ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSION
Sanamed
burn
%tbsa
t lymphocytes
thickness of burns
% cd3+ly
% cd3+ cd4+ly
% cd3+cd8+ly
% cd3+hla-dr+ly
ratio % cd4 / cd8 lymphocytes
title ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSION
title_full ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSION
title_fullStr ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSION
title_full_unstemmed ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSION
title_short ANALYSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN BURNS OF VARYING DEGREES IN THE ASSESSMENT OF IMMMUNE SUPPRESSION
title_sort analysis of peripheral blood lymphocytes in burns of varying degrees in the assessment of immmune suppression
topic burn
%tbsa
t lymphocytes
thickness of burns
% cd3+ly
% cd3+ cd4+ly
% cd3+cd8+ly
% cd3+hla-dr+ly
ratio % cd4 / cd8 lymphocytes
url http://www.sanamed.rs/OJS/index.php/Sanamed/article/view/462/242
work_keys_str_mv AT selmaarslanagic analysisofperipheralbloodlymphocytesinburnsofvaryingdegreesintheassessmentofimmmunesuppression
AT jasenkokaramehic analysisofperipheralbloodlymphocytesinburnsofvaryingdegreesintheassessmentofimmmunesuppression