The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad city
Background: The free-form of vitamin D has been used by many researchers as an index of vitamin D status in health and disease. Several methods are there to estimate free, total, and even bioavailable vitamin D. Objective: The present work was carried out to measure free vitamin D using a special...
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| Format: | Article |
| Language: | English |
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College of Medicine, Al-Nahrain University
2021-06-01
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| Series: | Baghdad Journal of Biochemistry and Applied Biological Sciences |
| Subjects: | |
| Online Access: | https://bjbabs.org/index.php/bjbabs/article/view/41 |
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| author | Zainab Subber Ghassan Al-Shamma Hashim Hashim |
| author_facet | Zainab Subber Ghassan Al-Shamma Hashim Hashim |
| author_sort | Zainab Subber |
| collection | DOAJ |
| description | Background: The free-form of vitamin D has been used by many researchers as an index of vitamin D status in health and disease. Several methods are there to estimate free, total, and even bioavailable vitamin D.
Objective: The present work was carried out to measure free vitamin D using a special formula suggested by Bikle and Schwartz in 2019, which includes the vitamin D binding protein (VDBP). The results will be used to evaluate the vitamin D status in patients with type 2 diabetes mellitus (T2DM), and its relation to the disease progression.
Methods: Sixty-four patients with T2DM and 73 healthy subjects, all from Baghdad city, were enrolled in the current study from March to October 2020. For each participant, fasting blood glucose, hemoglobin (HbA1c), insulin resistance HOMA-IR, and body mass index (BMI) were measured in addition to the total vitamin D and VDBP. Moreover, free vitamin D was calculated by the formula of Bikle & Schwartz.
Results: There were highly significant correlations between total vitamin D and absolute values of free vitamin D or its percentage. The difference in total vitamin D was significant between patients and healthy controls with no significant change in VDBP, free and bio-available vitamin D, while free vitamin D% was higher in the patient’s group. Correlations between vitamin D and each of BMI, fasting glucose, HbA1c, and HOMA-IR were not significant; however, there was a negative correlation with BMI and fasting glucose in the healthy control subjects only. The Receiver Operating Characteristic (ROC) curve analysis of vitamin D in the diagnosis of diabetes mellitus was poor.
Conclusion: Total vitamin D can represent vitamin D status, but it cannot be used as a factor for diagnosing T2DM. However, it could be of importance to change the glycemic status. |
| first_indexed | 2024-12-24T19:08:07Z |
| format | Article |
| id | doaj.art-82d20c7b1eda435b9ecfaf4ef9eb246b |
| institution | Directory Open Access Journal |
| issn | 2706-9915 |
| language | English |
| last_indexed | 2024-12-24T19:08:07Z |
| publishDate | 2021-06-01 |
| publisher | College of Medicine, Al-Nahrain University |
| record_format | Article |
| series | Baghdad Journal of Biochemistry and Applied Biological Sciences |
| spelling | doaj.art-82d20c7b1eda435b9ecfaf4ef9eb246b2022-12-21T16:43:03ZengCollege of Medicine, Al-Nahrain UniversityBaghdad Journal of Biochemistry and Applied Biological Sciences2706-99152021-06-01202The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad cityZainab Subber0Ghassan Al-Shamma1Hashim Hashim2Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, IraqDepartment of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, IraqDepartment of Medicine, College of Medicine, Al-Nahrain University, Baghdad, IraqBackground: The free-form of vitamin D has been used by many researchers as an index of vitamin D status in health and disease. Several methods are there to estimate free, total, and even bioavailable vitamin D. Objective: The present work was carried out to measure free vitamin D using a special formula suggested by Bikle and Schwartz in 2019, which includes the vitamin D binding protein (VDBP). The results will be used to evaluate the vitamin D status in patients with type 2 diabetes mellitus (T2DM), and its relation to the disease progression. Methods: Sixty-four patients with T2DM and 73 healthy subjects, all from Baghdad city, were enrolled in the current study from March to October 2020. For each participant, fasting blood glucose, hemoglobin (HbA1c), insulin resistance HOMA-IR, and body mass index (BMI) were measured in addition to the total vitamin D and VDBP. Moreover, free vitamin D was calculated by the formula of Bikle & Schwartz. Results: There were highly significant correlations between total vitamin D and absolute values of free vitamin D or its percentage. The difference in total vitamin D was significant between patients and healthy controls with no significant change in VDBP, free and bio-available vitamin D, while free vitamin D% was higher in the patient’s group. Correlations between vitamin D and each of BMI, fasting glucose, HbA1c, and HOMA-IR were not significant; however, there was a negative correlation with BMI and fasting glucose in the healthy control subjects only. The Receiver Operating Characteristic (ROC) curve analysis of vitamin D in the diagnosis of diabetes mellitus was poor. Conclusion: Total vitamin D can represent vitamin D status, but it cannot be used as a factor for diagnosing T2DM. However, it could be of importance to change the glycemic status.https://bjbabs.org/index.php/bjbabs/article/view/41Bikle methodBioavailable vitamin DFree vitamin DTotal vitamin DType 2 diabetes mellitusVermeulen method |
| spellingShingle | Zainab Subber Ghassan Al-Shamma Hashim Hashim The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad city Baghdad Journal of Biochemistry and Applied Biological Sciences Bikle method Bioavailable vitamin D Free vitamin D Total vitamin D Type 2 diabetes mellitus Vermeulen method |
| title | The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad city |
| title_full | The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad city |
| title_fullStr | The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad city |
| title_full_unstemmed | The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad city |
| title_short | The total and free vitamin D in type 2 diabetes mellitus patients in Baghdad city |
| title_sort | total and free vitamin d in type 2 diabetes mellitus patients in baghdad city |
| topic | Bikle method Bioavailable vitamin D Free vitamin D Total vitamin D Type 2 diabetes mellitus Vermeulen method |
| url | https://bjbabs.org/index.php/bjbabs/article/view/41 |
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