Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
In soil microbial ecology, the effects of environmental factors and their gradients, temporal changes or the response to specific experimental treatments of microbial communities can only be effectively analyzed using methods that address the structural differences among whole communities. Fingerpri...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2009-10-01
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| Series: | Plant, Soil and Environment |
| Subjects: | |
| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200910-0001_denaturing-gradient-gel-electrophoresis-as-a-fingerprinting-method-for-the-analysis-of-soil-microbial-communiti.php |
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| author | V. Valášková P. Baldrian |
| author_facet | V. Valášková P. Baldrian |
| author_sort | V. Valášková |
| collection | DOAJ |
| description | In soil microbial ecology, the effects of environmental factors and their gradients, temporal changes or the response to specific experimental treatments of microbial communities can only be effectively analyzed using methods that address the structural differences among whole communities. Fingerprinting methods are the most appropriate technique for this task when multiple samples must be analyzed. Among the methods currently used to compare microbial communities based on nucleic acid sequences, the techniques based on differences in the melting properties of double-stranded molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely used. Their main advantage is that they provide the possibility to further analyze whole sequences contained in fingerprints using molecular methods. In addition to the analysis of microbial communities based on DNA extracted from soils, DGGE/TGGE can also be used for the assessment of the active part of the community based on the analysis of RNA-derived sequences or for the analysis of sequences of functional genes encoding for proteins involved in important soil processes. |
| first_indexed | 2024-04-10T08:12:07Z |
| format | Article |
| id | doaj.art-82d25b318a49482ea72736ad969692f4 |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:12:07Z |
| publishDate | 2009-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-82d25b318a49482ea72736ad969692f42023-02-23T03:45:31ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682009-10-01551041342310.17221/132/2009-PSEpse-200910-0001Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communitiesV. Valášková0P. Baldrian1Laboratory of Environmental Microbiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, Prague, Czech RepublicLaboratory of Environmental Microbiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, Prague, Czech RepublicIn soil microbial ecology, the effects of environmental factors and their gradients, temporal changes or the response to specific experimental treatments of microbial communities can only be effectively analyzed using methods that address the structural differences among whole communities. Fingerprinting methods are the most appropriate technique for this task when multiple samples must be analyzed. Among the methods currently used to compare microbial communities based on nucleic acid sequences, the techniques based on differences in the melting properties of double-stranded molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely used. Their main advantage is that they provide the possibility to further analyze whole sequences contained in fingerprints using molecular methods. In addition to the analysis of microbial communities based on DNA extracted from soils, DGGE/TGGE can also be used for the assessment of the active part of the community based on the analysis of RNA-derived sequences or for the analysis of sequences of functional genes encoding for proteins involved in important soil processes.https://pse.agriculturejournals.cz/artkey/pse-200910-0001_denaturing-gradient-gel-electrophoresis-as-a-fingerprinting-method-for-the-analysis-of-soil-microbial-communiti.phpdggebacteriafungimicrobial communitysoil ecologytgge |
| spellingShingle | V. Valášková P. Baldrian Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities Plant, Soil and Environment dgge bacteria fungi microbial community soil ecology tgge |
| title | Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities |
| title_full | Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities |
| title_fullStr | Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities |
| title_full_unstemmed | Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities |
| title_short | Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities |
| title_sort | denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities |
| topic | dgge bacteria fungi microbial community soil ecology tgge |
| url | https://pse.agriculturejournals.cz/artkey/pse-200910-0001_denaturing-gradient-gel-electrophoresis-as-a-fingerprinting-method-for-the-analysis-of-soil-microbial-communiti.php |
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