Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms
Biofilms formed by antibiotic-resistant bacteria in wound beds present unique challenges in terms of treating chronic wound infections; biofilms formed by one or more than one bacterial species are often involved. In this work, the in vitro anti-biofilm activity of a novel electrochemical bandage (e...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207521000137 |
| _version_ | 1819172942405173248 |
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| author | Yash S. Raval Abdelrhman Mohamed Laure Flurin Jayawant N. Mandrekar Kerryl E. Greenwood Quaintance Haluk Beyenal Robin Patel |
| author_facet | Yash S. Raval Abdelrhman Mohamed Laure Flurin Jayawant N. Mandrekar Kerryl E. Greenwood Quaintance Haluk Beyenal Robin Patel |
| author_sort | Yash S. Raval |
| collection | DOAJ |
| description | Biofilms formed by antibiotic-resistant bacteria in wound beds present unique challenges in terms of treating chronic wound infections; biofilms formed by one or more than one bacterial species are often involved. In this work, the in vitro anti-biofilm activity of a novel electrochemical bandage (e-bandage) composed of carbon fabric and controlled by a wearable potentiostat, designed to continuously deliver low amounts of hydrogen peroxide (H2O2) was evaluated against 34 mono-species and 12 dual-species membrane bacterial biofilms formed by Staphylococcus aureus, S. epidermidis, Enterococcus faecium, E. faecalis, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Cutibacterium acnes, and Bacteroides fragilis. Biofilms were grown on polycarbonate membranes placed atop agar plates. An e-bandage, which electrochemically reduces dissolved oxygen to H2O2 when polarized at −0.6 VAg/AgCl, was then placed atop each membrane biofilm and polarized continuously for 12, 24, and 48 h using a wearable potentiostat. Time-dependent decreases in viable CFU counts of all mono- and dual-species biofilms were observed after e-bandage treatment. 48 h of e-bandage treatment resulted in an average reduction of 8.17 ± 0.40 and 7.99 ± 0.32 log10 CFU/cm2 for mono- and dual-species biofilms, respectively. Results suggest that the described H2O2 producing e-bandage can reduce in vitro viable cell counts of biofilms grown either in mono- or dual-species forms, and should be further developed as a potential antibiotic-free treatment strategy for treating chronic wound infections. |
| first_indexed | 2024-12-22T20:15:12Z |
| format | Article |
| id | doaj.art-82d3710444004dc28b426b21dc79ba18 |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-12-22T20:15:12Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-82d3710444004dc28b426b21dc79ba182022-12-21T18:13:59ZengElsevierBiofilm2590-20752021-12-013100055Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilmsYash S. Raval0Abdelrhman Mohamed1Laure Flurin2Jayawant N. Mandrekar3Kerryl E. Greenwood Quaintance4Haluk Beyenal5Robin Patel6Division of Clinical Microbiology, Mayo Clinic, Rochester, MN, USAThe Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USADivision of Clinical Microbiology, Mayo Clinic, Rochester, MN, USADepartment of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, USADivision of Clinical Microbiology, Mayo Clinic, Rochester, MN, USAThe Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USADivision of Clinical Microbiology, Mayo Clinic, Rochester, MN, USA; Division of Infectious Diseases, Mayo Clinic, Rochester, MN, USA; Corresponding author. Division of Clinical Microbiology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.Biofilms formed by antibiotic-resistant bacteria in wound beds present unique challenges in terms of treating chronic wound infections; biofilms formed by one or more than one bacterial species are often involved. In this work, the in vitro anti-biofilm activity of a novel electrochemical bandage (e-bandage) composed of carbon fabric and controlled by a wearable potentiostat, designed to continuously deliver low amounts of hydrogen peroxide (H2O2) was evaluated against 34 mono-species and 12 dual-species membrane bacterial biofilms formed by Staphylococcus aureus, S. epidermidis, Enterococcus faecium, E. faecalis, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Cutibacterium acnes, and Bacteroides fragilis. Biofilms were grown on polycarbonate membranes placed atop agar plates. An e-bandage, which electrochemically reduces dissolved oxygen to H2O2 when polarized at −0.6 VAg/AgCl, was then placed atop each membrane biofilm and polarized continuously for 12, 24, and 48 h using a wearable potentiostat. Time-dependent decreases in viable CFU counts of all mono- and dual-species biofilms were observed after e-bandage treatment. 48 h of e-bandage treatment resulted in an average reduction of 8.17 ± 0.40 and 7.99 ± 0.32 log10 CFU/cm2 for mono- and dual-species biofilms, respectively. Results suggest that the described H2O2 producing e-bandage can reduce in vitro viable cell counts of biofilms grown either in mono- or dual-species forms, and should be further developed as a potential antibiotic-free treatment strategy for treating chronic wound infections.http://www.sciencedirect.com/science/article/pii/S2590207521000137Electrochemical bandageHydrogen peroxideMembrane biofilmAnti-biofilmWound infections |
| spellingShingle | Yash S. Raval Abdelrhman Mohamed Laure Flurin Jayawant N. Mandrekar Kerryl E. Greenwood Quaintance Haluk Beyenal Robin Patel Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms Biofilm Electrochemical bandage Hydrogen peroxide Membrane biofilm Anti-biofilm Wound infections |
| title | Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms |
| title_full | Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms |
| title_fullStr | Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms |
| title_full_unstemmed | Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms |
| title_short | Hydrogen-peroxide generating electrochemical bandage is active in vitro against mono- and dual-species biofilms |
| title_sort | hydrogen peroxide generating electrochemical bandage is active in vitro against mono and dual species biofilms |
| topic | Electrochemical bandage Hydrogen peroxide Membrane biofilm Anti-biofilm Wound infections |
| url | http://www.sciencedirect.com/science/article/pii/S2590207521000137 |
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