A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration
Abstract Cellular senescence is a major hurdle for primary cell-based tissue engineering and regenerative medicine. Telomere erosion, oxidative stress, the expression of oncogenes and the loss of tumor suppressor genes all may account for the cellular senescence process with the involvement of vario...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Cell & Bioscience |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13578-018-0264-9 |
| _version_ | 1819034357357084672 |
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| author | Yiming Wang Song Chen Zuoqin Yan Ming Pei |
| author_facet | Yiming Wang Song Chen Zuoqin Yan Ming Pei |
| author_sort | Yiming Wang |
| collection | DOAJ |
| description | Abstract Cellular senescence is a major hurdle for primary cell-based tissue engineering and regenerative medicine. Telomere erosion, oxidative stress, the expression of oncogenes and the loss of tumor suppressor genes all may account for the cellular senescence process with the involvement of various signaling pathways. To establish immortalized cell lines for research and clinical use, strategies have been applied including internal genomic or external matrix microenvironment modification. Considering the potential risks of malignant transformation and tumorigenesis of genetic manipulation, environmental modification methods, especially the decellularized cell-deposited extracellular matrix (dECM)-based preconditioning strategy, appear to be promising for tissue engineering-aimed cell immortalization. Due to few review articles focusing on this topic, this review provides a summary of cell senescence and immortalization and discusses advantages and limitations of tissue engineering and regeneration with the use of immortalized cells as well as a potential rejuvenation strategy through combination with the dECM approach. |
| first_indexed | 2024-12-21T07:32:27Z |
| format | Article |
| id | doaj.art-82d37a6954f94df7b280497f3344fe00 |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-12-21T07:32:27Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-82d37a6954f94df7b280497f3344fe002022-12-21T19:11:31ZengBMCCell & Bioscience2045-37012019-01-019112110.1186/s13578-018-0264-9A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regenerationYiming Wang0Song Chen1Zuoqin Yan2Ming Pei3Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia UniversityDepartment of Orthopaedics, Chengdu Military General HospitalDepartment of Orthopaedics, Zhongshan Hospital of Fudan UniversityStem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia UniversityAbstract Cellular senescence is a major hurdle for primary cell-based tissue engineering and regenerative medicine. Telomere erosion, oxidative stress, the expression of oncogenes and the loss of tumor suppressor genes all may account for the cellular senescence process with the involvement of various signaling pathways. To establish immortalized cell lines for research and clinical use, strategies have been applied including internal genomic or external matrix microenvironment modification. Considering the potential risks of malignant transformation and tumorigenesis of genetic manipulation, environmental modification methods, especially the decellularized cell-deposited extracellular matrix (dECM)-based preconditioning strategy, appear to be promising for tissue engineering-aimed cell immortalization. Due to few review articles focusing on this topic, this review provides a summary of cell senescence and immortalization and discusses advantages and limitations of tissue engineering and regeneration with the use of immortalized cells as well as a potential rejuvenation strategy through combination with the dECM approach.http://link.springer.com/article/10.1186/s13578-018-0264-9Cell senescenceDecellularized cell-deposited extracellular matrixDifferentiationImmortalizationProliferationSV40 |
| spellingShingle | Yiming Wang Song Chen Zuoqin Yan Ming Pei A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration Cell & Bioscience Cell senescence Decellularized cell-deposited extracellular matrix Differentiation Immortalization Proliferation SV40 |
| title | A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration |
| title_full | A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration |
| title_fullStr | A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration |
| title_full_unstemmed | A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration |
| title_short | A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration |
| title_sort | prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration |
| topic | Cell senescence Decellularized cell-deposited extracellular matrix Differentiation Immortalization Proliferation SV40 |
| url | http://link.springer.com/article/10.1186/s13578-018-0264-9 |
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