The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells
5-fluorouracil (5-FU) is an anticancer drug used to inhibit the proliferation of many different tumor cells. Since severe events are associated with this compound, its combination with different anticancer drugs or adjuvants would allow the use of a significantly lower dose of 5-FU. In this study, w...
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2020-04-01
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| author | Adrian Bogdan Țigu Vlad-Alexandru Toma Augustin Cătălin Moț Ancuța Jurj Cristian Silviu Moldovan Eva Fischer-Fodor Ioana Berindan-Neagoe Marcel Pârvu |
| author_facet | Adrian Bogdan Țigu Vlad-Alexandru Toma Augustin Cătălin Moț Ancuța Jurj Cristian Silviu Moldovan Eva Fischer-Fodor Ioana Berindan-Neagoe Marcel Pârvu |
| author_sort | Adrian Bogdan Țigu |
| collection | DOAJ |
| description | 5-fluorouracil (5-FU) is an anticancer drug used to inhibit the proliferation of many different tumor cells. Since severe events are associated with this compound, its combination with different anticancer drugs or adjuvants would allow the use of a significantly lower dose of 5-FU. In this study, we highlighted that the combination of allicin with 5-FU inhibited the cell migration and proliferation of colorectal and lung cancer cells. 5-FU inhibited cell growth with a similar inhibitory concentration for both normal and tumor cells (~200µM), while allicin showed different inhibitory concentrations. With an IC50 of 8.625 µM, lung cancer cells were the most sensitive to allicin. Compared to 5-FU and allicin single-agent treatments, the co-treatment showed a reduced viability rate, with <i>p</i> < 0.05. The morphological changes were visible on all three cell lines, indicating that the treatment inhibited the proliferation of both normal and tumor cells. We highlighted different cell death mechanisms—apoptosis for lung cancer and a non-apoptotic cell death for colorectal cancer. The synergistic antitumor effect of 5-FU combined with allicin was visible against lung and colorectal carcinoma cells. Better results were obtained when a lower concentration of 5-FU was combined with allicin than the single-agent treatment at IC50. |
| first_indexed | 2024-03-10T20:18:28Z |
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| id | doaj.art-82d43fb11d294e19a2058dd08de8f7b5 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:18:28Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-82d43fb11d294e19a2058dd08de8f7b52023-11-19T22:24:47ZengMDPI AGMolecules1420-30492020-04-01258194710.3390/molecules25081947The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma CellsAdrian Bogdan Țigu0Vlad-Alexandru Toma1Augustin Cătălin Moț2Ancuța Jurj3Cristian Silviu Moldovan4Eva Fischer-Fodor5Ioana Berindan-Neagoe6Marcel Pârvu7MedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu”, 400349 Cluj-Napoca, RomaniaFaculty of Biology and Geology, Babeș-Bolyai University, 42 Republicii Street, 400015 Cluj-Napoca, RomaniaDepartment of Chemistry, Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University,11 Arany Janos Street, 400028 Cluj-Napoca, RomaniaThe Research Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400028 Cluj-Napoca, RomaniaMedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu”, 400349 Cluj-Napoca, RomaniaMedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu”, 400349 Cluj-Napoca, RomaniaMedFuture Research Center for Advanced Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu”, 400349 Cluj-Napoca, RomaniaFaculty of Biology and Geology, Babeș-Bolyai University, 42 Republicii Street, 400015 Cluj-Napoca, Romania5-fluorouracil (5-FU) is an anticancer drug used to inhibit the proliferation of many different tumor cells. Since severe events are associated with this compound, its combination with different anticancer drugs or adjuvants would allow the use of a significantly lower dose of 5-FU. In this study, we highlighted that the combination of allicin with 5-FU inhibited the cell migration and proliferation of colorectal and lung cancer cells. 5-FU inhibited cell growth with a similar inhibitory concentration for both normal and tumor cells (~200µM), while allicin showed different inhibitory concentrations. With an IC50 of 8.625 µM, lung cancer cells were the most sensitive to allicin. Compared to 5-FU and allicin single-agent treatments, the co-treatment showed a reduced viability rate, with <i>p</i> < 0.05. The morphological changes were visible on all three cell lines, indicating that the treatment inhibited the proliferation of both normal and tumor cells. We highlighted different cell death mechanisms—apoptosis for lung cancer and a non-apoptotic cell death for colorectal cancer. The synergistic antitumor effect of 5-FU combined with allicin was visible against lung and colorectal carcinoma cells. Better results were obtained when a lower concentration of 5-FU was combined with allicin than the single-agent treatment at IC50.https://www.mdpi.com/1420-3049/25/8/1947allicin5-FUapoptosiscolonysynergistic effectmigration |
| spellingShingle | Adrian Bogdan Țigu Vlad-Alexandru Toma Augustin Cătălin Moț Ancuța Jurj Cristian Silviu Moldovan Eva Fischer-Fodor Ioana Berindan-Neagoe Marcel Pârvu The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells Molecules allicin 5-FU apoptosis colony synergistic effect migration |
| title | The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells |
| title_full | The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells |
| title_fullStr | The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells |
| title_full_unstemmed | The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells |
| title_short | The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells |
| title_sort | synergistic antitumor effect of 5 fluorouracil combined with allicin against lung and colorectal carcinoma cells |
| topic | allicin 5-FU apoptosis colony synergistic effect migration |
| url | https://www.mdpi.com/1420-3049/25/8/1947 |
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