Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology
Near-infrared spectroscopy (NIRS) is known to be a rapid and non-destructive technique for process monitoring. Bioprocesses are usually complex, from both the chemical (ill-defined medium composition) and physical (multiphase matrix) aspects, which poses an additional challenge to the development o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/14/10/18941 |
| _version_ | 1811184717554253824 |
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| author | Elena Tamburini Maria Gabriella Marchetti Paola Pedrini |
| author_facet | Elena Tamburini Maria Gabriella Marchetti Paola Pedrini |
| author_sort | Elena Tamburini |
| collection | DOAJ |
| description | Near-infrared spectroscopy (NIRS) is known to be a rapid and non-destructive technique for process monitoring. Bioprocesses are usually complex, from both the chemical (ill-defined medium composition) and physical (multiphase matrix) aspects, which poses an additional challenge to the development of robust calibrations. We investigated the use of NIRS for on-line and in-line monitoring of cell, substrate and product concentrations, during aerobic and anaerobic bacterial fermentations, in different fermentation strategies. Calibration models were built up, then validated and used for the automated control of fermentation processes. The capability of NIR in-line to discriminate among differently shaped bacteria was tested. |
| first_indexed | 2024-04-11T13:17:13Z |
| format | Article |
| id | doaj.art-82d6474c335046b1916bfa951319c0bf |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:17:13Z |
| publishDate | 2014-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-82d6474c335046b1916bfa951319c0bf2022-12-22T04:22:21ZengMDPI AGSensors1424-82202014-10-011410189411895910.3390/s141018941s141018941Monitoring Key Parameters in Bioprocesses Using Near-Infrared TechnologyElena Tamburini0Maria Gabriella Marchetti1Paola Pedrini2Department of Life Science and Biotecnology, University of Ferrara, Via L. Borsari, 46 I-44121, ItalyDepartment of Life Science and Biotecnology, University of Ferrara, Via L. Borsari, 46 I-44121, ItalyDepartment of Life Science and Biotecnology, University of Ferrara, Via L. Borsari, 46 I-44121, ItalyNear-infrared spectroscopy (NIRS) is known to be a rapid and non-destructive technique for process monitoring. Bioprocesses are usually complex, from both the chemical (ill-defined medium composition) and physical (multiphase matrix) aspects, which poses an additional challenge to the development of robust calibrations. We investigated the use of NIRS for on-line and in-line monitoring of cell, substrate and product concentrations, during aerobic and anaerobic bacterial fermentations, in different fermentation strategies. Calibration models were built up, then validated and used for the automated control of fermentation processes. The capability of NIR in-line to discriminate among differently shaped bacteria was tested.http://www.mdpi.com/1424-8220/14/10/18941near-infrared spectroscopyfermentationon-line monitoringin-line monitoringcalibration curvesbioprocess control |
| spellingShingle | Elena Tamburini Maria Gabriella Marchetti Paola Pedrini Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology Sensors near-infrared spectroscopy fermentation on-line monitoring in-line monitoring calibration curves bioprocess control |
| title | Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology |
| title_full | Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology |
| title_fullStr | Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology |
| title_full_unstemmed | Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology |
| title_short | Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology |
| title_sort | monitoring key parameters in bioprocesses using near infrared technology |
| topic | near-infrared spectroscopy fermentation on-line monitoring in-line monitoring calibration curves bioprocess control |
| url | http://www.mdpi.com/1424-8220/14/10/18941 |
| work_keys_str_mv | AT elenatamburini monitoringkeyparametersinbioprocessesusingnearinfraredtechnology AT mariagabriellamarchetti monitoringkeyparametersinbioprocessesusingnearinfraredtechnology AT paolapedrini monitoringkeyparametersinbioprocessesusingnearinfraredtechnology |