ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds
Abstract The germination timing of seeds is of the utmost adaptive importance for plant populations. Light is one of the best characterized factors promoting seed germination in several species. The germination is also finely regulated by changes in hormones levels, mainly those of gibberellin (GA)...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-88874-5 |
| _version_ | 1819126616637308928 |
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| author | Tognacca Rocío Soledad Carabelli Monica Morelli Giorgio Ruberti Ida Botto Javier Francisco |
| author_facet | Tognacca Rocío Soledad Carabelli Monica Morelli Giorgio Ruberti Ida Botto Javier Francisco |
| author_sort | Tognacca Rocío Soledad |
| collection | DOAJ |
| description | Abstract The germination timing of seeds is of the utmost adaptive importance for plant populations. Light is one of the best characterized factors promoting seed germination in several species. The germination is also finely regulated by changes in hormones levels, mainly those of gibberellin (GA) and abscisic acid (ABA). Here, we performed physiological, pharmacological, and molecular analyses to uncover the role of ATHB2, an HD-ZIP II transcription factor, in germination of Arabidopsis seeds. Our study demonstrated that ATHB2 is a negative regulator and sustains the expression of transcription factors to block germination promoted by light. Besides, we found that ATHB2 increases ABA sensitivity. Moreover, ABA and auxin content in athb2-2 mutant is higher than wild-type in dry seeds, but the differences disappeared during the imbibition in darkness and the first hours of exposition to light, respectively. Some ABA and light transcription factors are up-regulated by ATHB2, such as ABI5, ABI3, XERICO, SOMNUS and PIL5/PIF1. In opposition, PIN7, an auxin transport, is down-regulated. The role of ATHB2 as a repressor of germination induced by light affecting the gemination timing, could have differential effects on the establishment of seedlings altering the competitiveness between crops and weeds in the field. |
| first_indexed | 2024-12-22T07:58:52Z |
| format | Article |
| id | doaj.art-82da4b4ef1a24933a0abadb0411f62da |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T07:58:52Z |
| publishDate | 2021-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-82da4b4ef1a24933a0abadb0411f62da2022-12-21T18:33:18ZengNature PortfolioScientific Reports2045-23222021-05-0111111310.1038/s41598-021-88874-5ATHB2 is a negative regulator of germination in Arabidopsis thaliana seedsTognacca Rocío Soledad0Carabelli Monica1Morelli Giorgio2Ruberti Ida3Botto Javier Francisco4Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA), Facultad de Agronomía, Universidad de Buenos AiresInstitute of Molecular Biology and Pathology, National Research CouncilResearch Centre for Genomics and Bioinformatics, Council for Agricultural Research and Economics (CREA)Institute of Molecular Biology and Pathology, National Research CouncilConsejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA), Facultad de Agronomía, Universidad de Buenos AiresAbstract The germination timing of seeds is of the utmost adaptive importance for plant populations. Light is one of the best characterized factors promoting seed germination in several species. The germination is also finely regulated by changes in hormones levels, mainly those of gibberellin (GA) and abscisic acid (ABA). Here, we performed physiological, pharmacological, and molecular analyses to uncover the role of ATHB2, an HD-ZIP II transcription factor, in germination of Arabidopsis seeds. Our study demonstrated that ATHB2 is a negative regulator and sustains the expression of transcription factors to block germination promoted by light. Besides, we found that ATHB2 increases ABA sensitivity. Moreover, ABA and auxin content in athb2-2 mutant is higher than wild-type in dry seeds, but the differences disappeared during the imbibition in darkness and the first hours of exposition to light, respectively. Some ABA and light transcription factors are up-regulated by ATHB2, such as ABI5, ABI3, XERICO, SOMNUS and PIL5/PIF1. In opposition, PIN7, an auxin transport, is down-regulated. The role of ATHB2 as a repressor of germination induced by light affecting the gemination timing, could have differential effects on the establishment of seedlings altering the competitiveness between crops and weeds in the field.https://doi.org/10.1038/s41598-021-88874-5 |
| spellingShingle | Tognacca Rocío Soledad Carabelli Monica Morelli Giorgio Ruberti Ida Botto Javier Francisco ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds Scientific Reports |
| title | ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds |
| title_full | ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds |
| title_fullStr | ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds |
| title_full_unstemmed | ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds |
| title_short | ATHB2 is a negative regulator of germination in Arabidopsis thaliana seeds |
| title_sort | athb2 is a negative regulator of germination in arabidopsis thaliana seeds |
| url | https://doi.org/10.1038/s41598-021-88874-5 |
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