Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression
Acute myocardial infarction (AMI) is myocardial necrosis caused by the persistent interruption of myocardial blood supply, which has high incidence rate and high mortality in middle-aged and elderly people in the worldwide. Biomarkers play an important role in the early diagnosis and treatment of AM...
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| Format: | Article |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.686116/full |
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| author | Wen Yang Li Sun Xun Cao Luyifei Li Xin Zhang Jianqian Li Hongyan Zhao Chengchuang Zhan Yanxiang Zang Tiankai Li Li Zhang Guangzhong Liu Weimin Li |
| author_facet | Wen Yang Li Sun Xun Cao Luyifei Li Xin Zhang Jianqian Li Hongyan Zhao Chengchuang Zhan Yanxiang Zang Tiankai Li Li Zhang Guangzhong Liu Weimin Li |
| author_sort | Wen Yang |
| collection | DOAJ |
| description | Acute myocardial infarction (AMI) is myocardial necrosis caused by the persistent interruption of myocardial blood supply, which has high incidence rate and high mortality in middle-aged and elderly people in the worldwide. Biomarkers play an important role in the early diagnosis and treatment of AMI. Recently, more and more researches confirmed that circRNA may be a potential diagnostic biomarker and therapeutic target for cardiovascular diseases. In this paper, a series of biological analyses were performed to find new effective circRNA biomarkers for AMI. Firstly, the expression levels of circRNAs in blood samples of patients with AMI and those with mild coronary stenosis were compared to reveal circRNAs which were involved in AMI. Then, circRNAs which were significant expressed abnormally in the blood samples of patients with AMI were selected from those circRNAs. Next, a ceRNA network was constructed based on interactions of circRNA, miRNA and mRNA through biological analyses to detect crucial circRNA associated with AMI. Finally, one circRNA was selected as candidate biomarker for AMI. To validate effectivity and efficiency of the candidate biomarker, fluorescence in situ hybridization, hypoxia model of human cardiomyocytes, and knockdown and overexpression analyses were performed on candidate circRNA biomarker. In conclusion, experimental results demonstrated that the candidate circRNA was an effective biomarker for diagnosis and therapy of AMI. |
| first_indexed | 2024-12-19T12:21:37Z |
| format | Article |
| id | doaj.art-82dbd16e6cbc44538efdbc219673e246 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-19T12:21:37Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-82dbd16e6cbc44538efdbc219673e2462022-12-21T20:21:44ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-04-011210.3389/fgene.2021.686116686116Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA ExpressionWen Yang0Li Sun1Xun Cao2Luyifei Li3Xin Zhang4Jianqian Li5Hongyan Zhao6Chengchuang Zhan7Yanxiang Zang8Tiankai Li9Li Zhang10Guangzhong Liu11Weimin Li12Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, China University of Science and Technology, Hefei, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The People’s Hospital of Liaoning Province, Shenyang, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaDepartment of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, ChinaAcute myocardial infarction (AMI) is myocardial necrosis caused by the persistent interruption of myocardial blood supply, which has high incidence rate and high mortality in middle-aged and elderly people in the worldwide. Biomarkers play an important role in the early diagnosis and treatment of AMI. Recently, more and more researches confirmed that circRNA may be a potential diagnostic biomarker and therapeutic target for cardiovascular diseases. In this paper, a series of biological analyses were performed to find new effective circRNA biomarkers for AMI. Firstly, the expression levels of circRNAs in blood samples of patients with AMI and those with mild coronary stenosis were compared to reveal circRNAs which were involved in AMI. Then, circRNAs which were significant expressed abnormally in the blood samples of patients with AMI were selected from those circRNAs. Next, a ceRNA network was constructed based on interactions of circRNA, miRNA and mRNA through biological analyses to detect crucial circRNA associated with AMI. Finally, one circRNA was selected as candidate biomarker for AMI. To validate effectivity and efficiency of the candidate biomarker, fluorescence in situ hybridization, hypoxia model of human cardiomyocytes, and knockdown and overexpression analyses were performed on candidate circRNA biomarker. In conclusion, experimental results demonstrated that the candidate circRNA was an effective biomarker for diagnosis and therapy of AMI.https://www.frontiersin.org/articles/10.3389/fgene.2021.686116/fullcircRNA1AMI 2microarray 3bioinformatics 4circRNA_1047615 |
| spellingShingle | Wen Yang Li Sun Xun Cao Luyifei Li Xin Zhang Jianqian Li Hongyan Zhao Chengchuang Zhan Yanxiang Zang Tiankai Li Li Zhang Guangzhong Liu Weimin Li Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression Frontiers in Genetics circRNA1 AMI 2 microarray 3 bioinformatics 4 circRNA_1047615 |
| title | Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression |
| title_full | Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression |
| title_fullStr | Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression |
| title_full_unstemmed | Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression |
| title_short | Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression |
| title_sort | detection of circrna biomarker for acute myocardial infarction based on system biological analysis of rna expression |
| topic | circRNA1 AMI 2 microarray 3 bioinformatics 4 circRNA_1047615 |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.686116/full |
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