Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc
Background: Degeneration of the intervertebral disc (IVD) is caused by disturbances in metabolic processes, which lead to structural disorders. The aim of this report is to analyze metabolic disorders in the degeneration process by comparing control discs with degenerated discs. In our research on t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/10572 |
| _version_ | 1797591638716973056 |
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| author | Beata Toczylowska Michal Woznica Elzbieta Zieminska Leszek Krolicki |
| author_facet | Beata Toczylowska Michal Woznica Elzbieta Zieminska Leszek Krolicki |
| author_sort | Beata Toczylowska |
| collection | DOAJ |
| description | Background: Degeneration of the intervertebral disc (IVD) is caused by disturbances in metabolic processes, which lead to structural disorders. The aim of this report is to analyze metabolic disorders in the degeneration process by comparing control discs with degenerated discs. In our research on the nucleus pulposus (NP), we used NMR spectroscopy of extracts of hydrophilic and hydrophobic compounds of the tissue. Methods: Nuclear magnetic resonance (NMR) spectroscopy allows the study of biochemistry and cellular metabolism in vitro. Hydrophilic and hydrophobic compounds were extracted from the NP of the intervertebral disc. In the NMR spectra, metabolites were identified and quantitatively analyzed. The results of our research indicate disturbances in the biosynthesis and metabolism of cholesterol, the biosynthesis and degradation of various fatty acid groups, ketone bodies, or lysine, and the metabolism of glycerophospholipids, purines, glycine, inositol, galactose, alanine, glutamate, and pyruvate in the biosynthesis of valine and isoleucine, leucine. All these disorders indicate pathomechanisms related to oxidative stress, energy, neurotransmission disturbances, and disturbances in the structure and functioning of cell membranes, inflammation, or chronic pain generators. Conclusions: NMR spectroscopy allows the identification of metabolites differentiating surgical from nonsurgical discs. These data may provide guidance in in vivo MRS studies in assessing the severity of lesions of the disc. |
| first_indexed | 2024-03-11T01:40:09Z |
| format | Article |
| id | doaj.art-82dbe196bca14397a254b9ccd5091f9d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:40:09Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-82dbe196bca14397a254b9ccd5091f9d2023-11-18T16:40:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131057210.3390/ijms241310572Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral DiscBeata Toczylowska0Michal Woznica1Elzbieta Zieminska2Leszek Krolicki3Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 02-109 Warsaw, PolandSpinal Unit, 7th Navy Hospital, 80-305 Gdansk, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, 02-106 Warsaw, PolandDepartment of Nuclear Medicine, Warsaw Medical University, 02-091 Warsaw, PolandBackground: Degeneration of the intervertebral disc (IVD) is caused by disturbances in metabolic processes, which lead to structural disorders. The aim of this report is to analyze metabolic disorders in the degeneration process by comparing control discs with degenerated discs. In our research on the nucleus pulposus (NP), we used NMR spectroscopy of extracts of hydrophilic and hydrophobic compounds of the tissue. Methods: Nuclear magnetic resonance (NMR) spectroscopy allows the study of biochemistry and cellular metabolism in vitro. Hydrophilic and hydrophobic compounds were extracted from the NP of the intervertebral disc. In the NMR spectra, metabolites were identified and quantitatively analyzed. The results of our research indicate disturbances in the biosynthesis and metabolism of cholesterol, the biosynthesis and degradation of various fatty acid groups, ketone bodies, or lysine, and the metabolism of glycerophospholipids, purines, glycine, inositol, galactose, alanine, glutamate, and pyruvate in the biosynthesis of valine and isoleucine, leucine. All these disorders indicate pathomechanisms related to oxidative stress, energy, neurotransmission disturbances, and disturbances in the structure and functioning of cell membranes, inflammation, or chronic pain generators. Conclusions: NMR spectroscopy allows the identification of metabolites differentiating surgical from nonsurgical discs. These data may provide guidance in in vivo MRS studies in assessing the severity of lesions of the disc.https://www.mdpi.com/1422-0067/24/13/10572nucleus pulposusmetabolic profilesNMR spectroscopydehydrated intervertebral disc |
| spellingShingle | Beata Toczylowska Michal Woznica Elzbieta Zieminska Leszek Krolicki Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc International Journal of Molecular Sciences nucleus pulposus metabolic profiles NMR spectroscopy dehydrated intervertebral disc |
| title | Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc |
| title_full | Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc |
| title_fullStr | Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc |
| title_full_unstemmed | Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc |
| title_short | Metabolic Biomarkers Differentiate a Surgical Intervertebral Disc from a Nonsurgical Intervertebral Disc |
| title_sort | metabolic biomarkers differentiate a surgical intervertebral disc from a nonsurgical intervertebral disc |
| topic | nucleus pulposus metabolic profiles NMR spectroscopy dehydrated intervertebral disc |
| url | https://www.mdpi.com/1422-0067/24/13/10572 |
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