Autofluorescence: A screening test for mycotic infection in tissues
Fungal infection is a major health concern as the clinical features are not very distinctive. Lack of rapid diagnostic techniques results in delay in diagnosis, which may even culminate in a fatal outcome. The fact that many pathogenic fungal organisms autofluoresce in hematoxylin and eosin (H and E...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2008-04-01
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| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2008;volume=51;issue=2;spage=215;epage=217;aulast=Rao |
| _version_ | 1818511747985702912 |
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| author | Rao Shalinee Rajkumar Aarthi Ehtesham Md Prathiba D |
| author_facet | Rao Shalinee Rajkumar Aarthi Ehtesham Md Prathiba D |
| author_sort | Rao Shalinee |
| collection | DOAJ |
| description | Fungal infection is a major health concern as the clinical features are not very distinctive. Lack of rapid diagnostic techniques results in delay in diagnosis, which may even culminate in a fatal outcome. The fact that many pathogenic fungal organisms autofluoresce in hematoxylin and eosin (H and E)-stained sections under ultraviolet illumination led us to evaluate the role of autofluorescence as a rapid screening technique for fungal infections. The aim of the present study was to assess the value of autofluorescence as a screening method for detecting fungi on tissue sections and to compare the results of autofluorescence with conventional histochemical stains for fungi. Hematoxylin and eosin-stained slides of mycotic lesions were examined under fluorescent microscope and the findings were compared with results of Gomori′s methenamine silver and periodic acid-Schiff stains. We found fungal autofluorescence in 63 out of 64 cases studied, with a sensitivity of 97.8% and specificity of 100% in comparison with fungal stains. This was statistically significant (<i>P</i> < 0.05). We conclude that autofluorescence can be used as a rapid screening method for identification of fungi in tissue sections as it does not require any other specialized staining procedure |
| first_indexed | 2024-12-10T23:37:11Z |
| format | Article |
| id | doaj.art-82dc1939d4a74c49ae4d22e0796870cc |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-12-10T23:37:11Z |
| publishDate | 2008-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-82dc1939d4a74c49ae4d22e0796870cc2022-12-22T01:29:09ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292008-04-01512215217Autofluorescence: A screening test for mycotic infection in tissuesRao ShalineeRajkumar AarthiEhtesham MdPrathiba DFungal infection is a major health concern as the clinical features are not very distinctive. Lack of rapid diagnostic techniques results in delay in diagnosis, which may even culminate in a fatal outcome. The fact that many pathogenic fungal organisms autofluoresce in hematoxylin and eosin (H and E)-stained sections under ultraviolet illumination led us to evaluate the role of autofluorescence as a rapid screening technique for fungal infections. The aim of the present study was to assess the value of autofluorescence as a screening method for detecting fungi on tissue sections and to compare the results of autofluorescence with conventional histochemical stains for fungi. Hematoxylin and eosin-stained slides of mycotic lesions were examined under fluorescent microscope and the findings were compared with results of Gomori′s methenamine silver and periodic acid-Schiff stains. We found fungal autofluorescence in 63 out of 64 cases studied, with a sensitivity of 97.8% and specificity of 100% in comparison with fungal stains. This was statistically significant (<i>P</i> < 0.05). We conclude that autofluorescence can be used as a rapid screening method for identification of fungi in tissue sections as it does not require any other specialized staining procedurehttp://www.ijpmonline.org/article.asp?issn=0377-4929;year=2008;volume=51;issue=2;spage=215;epage=217;aulast=RaoAutofluorescencefungustissue sections |
| spellingShingle | Rao Shalinee Rajkumar Aarthi Ehtesham Md Prathiba D Autofluorescence: A screening test for mycotic infection in tissues Indian Journal of Pathology and Microbiology Autofluorescence fungus tissue sections |
| title | Autofluorescence: A screening test for mycotic infection in tissues |
| title_full | Autofluorescence: A screening test for mycotic infection in tissues |
| title_fullStr | Autofluorescence: A screening test for mycotic infection in tissues |
| title_full_unstemmed | Autofluorescence: A screening test for mycotic infection in tissues |
| title_short | Autofluorescence: A screening test for mycotic infection in tissues |
| title_sort | autofluorescence a screening test for mycotic infection in tissues |
| topic | Autofluorescence fungus tissue sections |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2008;volume=51;issue=2;spage=215;epage=217;aulast=Rao |
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