Lung microbiome in children with hematological malignancies and lower respiratory tract infections
BackgroundRespiratory infectious complications remain a major cause of morbidity and mortality in children with hematological malignancies. Knowledge regarding the lung microbiome in aforementioned children is limited.MethodsA prospective cohort was conducted, enrolling 16 children with hematologica...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.932709/full |
| _version_ | 1811272218554925056 |
|---|---|
| author | Yun Zhang Haonan Ning Wenyu Zheng Jing Liu Fuhai Li Junfei Chen |
| author_facet | Yun Zhang Haonan Ning Wenyu Zheng Jing Liu Fuhai Li Junfei Chen |
| author_sort | Yun Zhang |
| collection | DOAJ |
| description | BackgroundRespiratory infectious complications remain a major cause of morbidity and mortality in children with hematological malignancies. Knowledge regarding the lung microbiome in aforementioned children is limited.MethodsA prospective cohort was conducted, enrolling 16 children with hematological malignancies complicated with moderate-to-severe lower respiratory tract infections (LRTIs) versus 21 LRTI children with age, gender, weight, and infection severity matched, with no underlying malignancies, to evaluate the lung microbiome from bronchoalveolar lavage fluid samples in different groups.ResultsThe lung microbiome from children with hematological malignancies and LRTIs showed obviously decreased α and β diversity; increased microbial function in infectious disease:bacteria/parasite; drug resistance:antimicrobial and human pathogenesis than the control group; a significantly reduced proportion of Firmicutes, Bacteroidota, Actinobacteriota; increased Proteobacteria at the phylum level; and distinctly elevated Parabacteroides, Klebsiella, Grimontia, Escherichia_Shigella, unclassified_Enterobacteriaceae at the genus level than the control group. Furthermore, it was revealed that α diversity (Shannon), β diversity (Bray–Curtis dissimilarity), Proteobacteria at the phylum level, and unclassified_Enterobacteriaceae and Escherichia_Shigella at the genus level were significantly negatively associated with hospitalization course whereas Firmicutes at the phylum level was established positively correlated with the hospitalization course.ConclusionsChildren with hematological malignancies and LRTIs showed obviously decreased α and β diversity, significantly increased function in infectious disease pathogenesis, antimicrobial drug resistance, and unfavorable environment tolerance. Moreover, α diversity (Shannon), β diversity (Bray–Curtis dissimilarity), and Proteobacteria may be used as negative correlated predictors for hospitalization course in these children whereas Firmicutes may be utilized as a positive correlated predictor. |
| first_indexed | 2024-04-12T22:36:12Z |
| format | Article |
| id | doaj.art-82e0db4972464423a202d18440bfe9af |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T22:36:12Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-82e0db4972464423a202d18440bfe9af2022-12-22T03:13:51ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-09-011210.3389/fonc.2022.932709932709Lung microbiome in children with hematological malignancies and lower respiratory tract infectionsYun Zhang0Haonan Ning1Wenyu Zheng2Jing Liu3Fuhai Li4Junfei Chen5Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Pediatrics, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Pediatrics, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Biostatistics, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Pediatrics, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan, ChinaBackgroundRespiratory infectious complications remain a major cause of morbidity and mortality in children with hematological malignancies. Knowledge regarding the lung microbiome in aforementioned children is limited.MethodsA prospective cohort was conducted, enrolling 16 children with hematological malignancies complicated with moderate-to-severe lower respiratory tract infections (LRTIs) versus 21 LRTI children with age, gender, weight, and infection severity matched, with no underlying malignancies, to evaluate the lung microbiome from bronchoalveolar lavage fluid samples in different groups.ResultsThe lung microbiome from children with hematological malignancies and LRTIs showed obviously decreased α and β diversity; increased microbial function in infectious disease:bacteria/parasite; drug resistance:antimicrobial and human pathogenesis than the control group; a significantly reduced proportion of Firmicutes, Bacteroidota, Actinobacteriota; increased Proteobacteria at the phylum level; and distinctly elevated Parabacteroides, Klebsiella, Grimontia, Escherichia_Shigella, unclassified_Enterobacteriaceae at the genus level than the control group. Furthermore, it was revealed that α diversity (Shannon), β diversity (Bray–Curtis dissimilarity), Proteobacteria at the phylum level, and unclassified_Enterobacteriaceae and Escherichia_Shigella at the genus level were significantly negatively associated with hospitalization course whereas Firmicutes at the phylum level was established positively correlated with the hospitalization course.ConclusionsChildren with hematological malignancies and LRTIs showed obviously decreased α and β diversity, significantly increased function in infectious disease pathogenesis, antimicrobial drug resistance, and unfavorable environment tolerance. Moreover, α diversity (Shannon), β diversity (Bray–Curtis dissimilarity), and Proteobacteria may be used as negative correlated predictors for hospitalization course in these children whereas Firmicutes may be utilized as a positive correlated predictor.https://www.frontiersin.org/articles/10.3389/fonc.2022.932709/fulllung microbiomebronchoalveolar lavage fluidchildrenhematological malignancieslower respiratory tract infectiondrug resistance |
| spellingShingle | Yun Zhang Haonan Ning Wenyu Zheng Jing Liu Fuhai Li Junfei Chen Lung microbiome in children with hematological malignancies and lower respiratory tract infections Frontiers in Oncology lung microbiome bronchoalveolar lavage fluid children hematological malignancies lower respiratory tract infection drug resistance |
| title | Lung microbiome in children with hematological malignancies and lower respiratory tract infections |
| title_full | Lung microbiome in children with hematological malignancies and lower respiratory tract infections |
| title_fullStr | Lung microbiome in children with hematological malignancies and lower respiratory tract infections |
| title_full_unstemmed | Lung microbiome in children with hematological malignancies and lower respiratory tract infections |
| title_short | Lung microbiome in children with hematological malignancies and lower respiratory tract infections |
| title_sort | lung microbiome in children with hematological malignancies and lower respiratory tract infections |
| topic | lung microbiome bronchoalveolar lavage fluid children hematological malignancies lower respiratory tract infection drug resistance |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.932709/full |
| work_keys_str_mv | AT yunzhang lungmicrobiomeinchildrenwithhematologicalmalignanciesandlowerrespiratorytractinfections AT haonanning lungmicrobiomeinchildrenwithhematologicalmalignanciesandlowerrespiratorytractinfections AT wenyuzheng lungmicrobiomeinchildrenwithhematologicalmalignanciesandlowerrespiratorytractinfections AT jingliu lungmicrobiomeinchildrenwithhematologicalmalignanciesandlowerrespiratorytractinfections AT fuhaili lungmicrobiomeinchildrenwithhematologicalmalignanciesandlowerrespiratorytractinfections AT junfeichen lungmicrobiomeinchildrenwithhematologicalmalignanciesandlowerrespiratorytractinfections |