Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS
Red blood cells (RBCs) are routinely stored for 35 to 42 days in most countries. During storage, RBCs undergo biochemical and biophysical changes known as RBC storage lesion, which is influenced by alternative storage additive solutions (ASs). Metabolomic studies have been completed on RBCs stored i...
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| Format: | Article |
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MDPI AG
2018-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/4/923 |
| _version_ | 1818838454148005888 |
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| author | Yong Zhou Zhiyun Meng Hui Gan Ying Zheng Xiaoxia Zhu Zhuona Wu Jian Li Ruolan Gu Guifang Dou |
| author_facet | Yong Zhou Zhiyun Meng Hui Gan Ying Zheng Xiaoxia Zhu Zhuona Wu Jian Li Ruolan Gu Guifang Dou |
| author_sort | Yong Zhou |
| collection | DOAJ |
| description | Red blood cells (RBCs) are routinely stored for 35 to 42 days in most countries. During storage, RBCs undergo biochemical and biophysical changes known as RBC storage lesion, which is influenced by alternative storage additive solutions (ASs). Metabolomic studies have been completed on RBCs stored in a number of ASs, including SAGM, AS-1, AS-3, AS-5, AS-7, PAGGGM, and MAP. However, the reported metabolome analysis of laboratory-made MAP-stored RBCs was mainly focused on the time-dependent alterations in glycolytic intermediates during storage. In this study, we investigated the time-course of alterations in various small molecule metabolites in RBCs stored in commercially used MAP for 49 days using ultra-high performance liquid chromatography quadruple time-of-flight mass spectrometry (UPLC-QTOF-MS). These alterations indicated that RBC storage lesion is related to multiple pathways including glycolysis, pentose phosphate pathway, glutathione homeostasis, and purine metabolism. Thus, our findings might be useful for understanding the complexity of metabolic mechanisms of RBCs in vitro aging and encourage the deployment of systems biology methods to blood products in transfusion medicine. |
| first_indexed | 2024-12-19T03:38:39Z |
| format | Article |
| id | doaj.art-82e0e28bfdf742be81724911f94c2290 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T03:38:39Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-82e0e28bfdf742be81724911f94c22902022-12-21T20:37:19ZengMDPI AGMolecules1420-30492018-04-0123492310.3390/molecules23040923molecules23040923Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MSYong Zhou0Zhiyun Meng1Hui Gan2Ying Zheng3Xiaoxia Zhu4Zhuona Wu5Jian Li6Ruolan Gu7Guifang Dou8Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaDepartment of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, ChinaRed blood cells (RBCs) are routinely stored for 35 to 42 days in most countries. During storage, RBCs undergo biochemical and biophysical changes known as RBC storage lesion, which is influenced by alternative storage additive solutions (ASs). Metabolomic studies have been completed on RBCs stored in a number of ASs, including SAGM, AS-1, AS-3, AS-5, AS-7, PAGGGM, and MAP. However, the reported metabolome analysis of laboratory-made MAP-stored RBCs was mainly focused on the time-dependent alterations in glycolytic intermediates during storage. In this study, we investigated the time-course of alterations in various small molecule metabolites in RBCs stored in commercially used MAP for 49 days using ultra-high performance liquid chromatography quadruple time-of-flight mass spectrometry (UPLC-QTOF-MS). These alterations indicated that RBC storage lesion is related to multiple pathways including glycolysis, pentose phosphate pathway, glutathione homeostasis, and purine metabolism. Thus, our findings might be useful for understanding the complexity of metabolic mechanisms of RBCs in vitro aging and encourage the deployment of systems biology methods to blood products in transfusion medicine.http://www.mdpi.com/1420-3049/23/4/923red blood cellsMAPUPLC-QTOF-MSmetabolomicsoxidative stressstorage lesion |
| spellingShingle | Yong Zhou Zhiyun Meng Hui Gan Ying Zheng Xiaoxia Zhu Zhuona Wu Jian Li Ruolan Gu Guifang Dou Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS Molecules red blood cells MAP UPLC-QTOF-MS metabolomics oxidative stress storage lesion |
| title | Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS |
| title_full | Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS |
| title_fullStr | Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS |
| title_full_unstemmed | Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS |
| title_short | Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS |
| title_sort | time course investigation of small molecule metabolites in map stored red blood cells using uplc qtof ms |
| topic | red blood cells MAP UPLC-QTOF-MS metabolomics oxidative stress storage lesion |
| url | http://www.mdpi.com/1420-3049/23/4/923 |
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