Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets
Zinc is an essential element, a cofactor of many enzymes, and performs catalytic, structural and regulatory functions. Once in the gastrointestinal tract, zinc can interact with food constituents. Phytic acid, the major phosphorus storage in plants, limits zinc availability from animal feeds due to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/3/400 |
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| author | Ana Margarida Pereira Margarida Guedes Elisabete Matos Edgar Pinto Agostinho A. Almeida Marcela A. Segundo Alexandra Correia Manuel Vilanova António J. M. Fonseca Ana Rita J. Cabrita |
| author_facet | Ana Margarida Pereira Margarida Guedes Elisabete Matos Edgar Pinto Agostinho A. Almeida Marcela A. Segundo Alexandra Correia Manuel Vilanova António J. M. Fonseca Ana Rita J. Cabrita |
| author_sort | Ana Margarida Pereira |
| collection | DOAJ |
| description | Zinc is an essential element, a cofactor of many enzymes, and performs catalytic, structural and regulatory functions. Once in the gastrointestinal tract, zinc can interact with food constituents. Phytic acid, the major phosphorus storage in plants, limits zinc availability from animal feeds due to the formation of insoluble complexes with phytates. This study tested the effect of supplemental zinc source (zinc sulfate and a chelate zinc proteinate) and the addition of exogenous enzymes from a solid-state fermentation product of <i>Aspergillus niger </i>to a high phytate diet. The study was designed according to three Latin Squares 4 × 4 with a 2 × 2 factorial arrangement of treatments, with four periods, four diets, and 12 young adult Beagles. Periods lasted 5 weeks each. Diets were supplemented with 75 mg/kg of zinc sulfate (IZ) or zinc proteinate (OZ), and without or with 200 mg/kg of exogenous enzymes (IZ+, OZ+). Results showed that zinc proteinate increased the bioavailability of phosphorus, yet the zinc biomarkers remained unaffected by the zinc source, with the exception of lymphocyte subsets that benefit from zinc proteinate. The use of exogenous enzymes did not affect zinc availability nor nutrient and energy digestibility. |
| first_indexed | 2024-12-13T00:45:27Z |
| format | Article |
| id | doaj.art-82e135696ffa4df2b078bb2f20a7f9ec |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-13T00:45:27Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-82e135696ffa4df2b078bb2f20a7f9ec2022-12-22T00:05:02ZengMDPI AGAnimals2076-26152020-02-0110340010.3390/ani10030400ani10030400Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate DietsAna Margarida Pereira0Margarida Guedes1Elisabete Matos2Edgar Pinto3Agostinho A. Almeida4Marcela A. Segundo5Alexandra Correia6Manuel Vilanova7António J. M. Fonseca8Ana Rita J. Cabrita9LAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira nº 228, 4050-313 Porto, PortugalLAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira nº 228, 4050-313 Porto, PortugalSORGAL, Sociedade de Óleos e Rações S.A., Estrada Nacional 109 Lugar da Pardala, 3880-728 S. João Ovar, PortugalLAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira nº 228, 4050-313 Porto, PortugalLAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira nº 228, 4050-313 Porto, PortugalLAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira nº 228, 4050-313 Porto, Portugali3S/IBMC – Instituto de Investigação e Inovação em Saúde/Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Alfredo Allen, 4200-135 Porto, Portugali3S/IBMC – Instituto de Investigação e Inovação em Saúde/Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Alfredo Allen, 4200-135 Porto, PortugalLAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira nº 228, 4050-313 Porto, PortugalLAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira nº 228, 4050-313 Porto, PortugalZinc is an essential element, a cofactor of many enzymes, and performs catalytic, structural and regulatory functions. Once in the gastrointestinal tract, zinc can interact with food constituents. Phytic acid, the major phosphorus storage in plants, limits zinc availability from animal feeds due to the formation of insoluble complexes with phytates. This study tested the effect of supplemental zinc source (zinc sulfate and a chelate zinc proteinate) and the addition of exogenous enzymes from a solid-state fermentation product of <i>Aspergillus niger </i>to a high phytate diet. The study was designed according to three Latin Squares 4 × 4 with a 2 × 2 factorial arrangement of treatments, with four periods, four diets, and 12 young adult Beagles. Periods lasted 5 weeks each. Diets were supplemented with 75 mg/kg of zinc sulfate (IZ) or zinc proteinate (OZ), and without or with 200 mg/kg of exogenous enzymes (IZ+, OZ+). Results showed that zinc proteinate increased the bioavailability of phosphorus, yet the zinc biomarkers remained unaffected by the zinc source, with the exception of lymphocyte subsets that benefit from zinc proteinate. The use of exogenous enzymes did not affect zinc availability nor nutrient and energy digestibility.https://www.mdpi.com/2076-2615/10/3/400zinc sulfatezinc proteinatedogssolid-state fermentation product of <i>aspergillus niger</i>phytates |
| spellingShingle | Ana Margarida Pereira Margarida Guedes Elisabete Matos Edgar Pinto Agostinho A. Almeida Marcela A. Segundo Alexandra Correia Manuel Vilanova António J. M. Fonseca Ana Rita J. Cabrita Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets Animals zinc sulfate zinc proteinate dogs solid-state fermentation product of <i>aspergillus niger</i> phytates |
| title | Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets |
| title_full | Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets |
| title_fullStr | Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets |
| title_full_unstemmed | Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets |
| title_short | Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets |
| title_sort | effect of zinc source and exogenous enzymes supplementation on zinc status in dogs fed high phytate diets |
| topic | zinc sulfate zinc proteinate dogs solid-state fermentation product of <i>aspergillus niger</i> phytates |
| url | https://www.mdpi.com/2076-2615/10/3/400 |
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