Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy
Abstract The menopause can lead to epidermal changes that are alleviated by hormone replacement therapy (HRT). We hypothesise that these changes could relate to altered ceramide production, and that oestrogen may have a role in keratinocyte ceramide metabolism. White Caucasian women were recruited i...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26095-0 |
| _version_ | 1828266129194221568 |
|---|---|
| author | Alexandra C. Kendall Suzanne M. Pilkington Jonathan R. Wray Victoria L. Newton Christopher E. M. Griffiths Mike Bell Rachel E. B. Watson Anna Nicolaou |
| author_facet | Alexandra C. Kendall Suzanne M. Pilkington Jonathan R. Wray Victoria L. Newton Christopher E. M. Griffiths Mike Bell Rachel E. B. Watson Anna Nicolaou |
| author_sort | Alexandra C. Kendall |
| collection | DOAJ |
| description | Abstract The menopause can lead to epidermal changes that are alleviated by hormone replacement therapy (HRT). We hypothesise that these changes could relate to altered ceramide production, and that oestrogen may have a role in keratinocyte ceramide metabolism. White Caucasian women were recruited into three groups: pre-menopausal (n = 7), post-menopausal (n = 11) and post-menopausal taking HRT (n = 10). Blood samples were assessed for hormone levels, transepidermal water loss was measured to assess skin barrier function, and stratum corneum lipids were sampled from photoprotected buttock skin. Ceramides and sphingomyelins were analysed by ultraperformance liquid chromatography with electrospray ionisation and tandem mass spectrometry. Post-menopausal stratum corneum contained lower levels of ceramides, with shorter average length; changes that were not evident in the HRT group. Serum oestradiol correlated with ceramide abundance and length. Ceramides had shorter sphingoid bases, indicating altered de novo ceramide biosynthesis. Additionally, post-menopausal women had higher sphingomyelin levels, suggesting a possible effect on the hydrolysis pathway. Treatment of primary human keratinocytes with oestradiol (10 nM) increased production of CER[NS] and CER[NDS] ceramides, confirming an effect of oestrogen on cutaneous ceramide metabolism. Taken together, these data show perturbed stratum corneum lipids post-menopause, and a role for oestrogen in ceramide production. |
| first_indexed | 2024-04-13T04:48:03Z |
| format | Article |
| id | doaj.art-82e2ea033c4f431a8e806576b37aa657 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T04:48:03Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-82e2ea033c4f431a8e806576b37aa6572022-12-22T03:01:47ZengNature PortfolioScientific Reports2045-23222022-12-0112111510.1038/s41598-022-26095-0Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapyAlexandra C. Kendall0Suzanne M. Pilkington1Jonathan R. Wray2Victoria L. Newton3Christopher E. M. Griffiths4Mike Bell5Rachel E. B. Watson6Anna Nicolaou7Laboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, The University of ManchesterDivision of Musculoskeletal and Dermatological Sciences, Centre for Dermatology Research, School of Biological Sciences, Faculty of Biology, Medicine, and Health, The University of ManchesterLaboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, The University of ManchesterThe No7 Beauty Company, Boots UK LtdDivision of Musculoskeletal and Dermatological Sciences, Centre for Dermatology Research, School of Biological Sciences, Faculty of Biology, Medicine, and Health, The University of ManchesterThe No7 Beauty Company, Boots UK LtdDivision of Musculoskeletal and Dermatological Sciences, Centre for Dermatology Research, School of Biological Sciences, Faculty of Biology, Medicine, and Health, The University of ManchesterLaboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, The University of ManchesterAbstract The menopause can lead to epidermal changes that are alleviated by hormone replacement therapy (HRT). We hypothesise that these changes could relate to altered ceramide production, and that oestrogen may have a role in keratinocyte ceramide metabolism. White Caucasian women were recruited into three groups: pre-menopausal (n = 7), post-menopausal (n = 11) and post-menopausal taking HRT (n = 10). Blood samples were assessed for hormone levels, transepidermal water loss was measured to assess skin barrier function, and stratum corneum lipids were sampled from photoprotected buttock skin. Ceramides and sphingomyelins were analysed by ultraperformance liquid chromatography with electrospray ionisation and tandem mass spectrometry. Post-menopausal stratum corneum contained lower levels of ceramides, with shorter average length; changes that were not evident in the HRT group. Serum oestradiol correlated with ceramide abundance and length. Ceramides had shorter sphingoid bases, indicating altered de novo ceramide biosynthesis. Additionally, post-menopausal women had higher sphingomyelin levels, suggesting a possible effect on the hydrolysis pathway. Treatment of primary human keratinocytes with oestradiol (10 nM) increased production of CER[NS] and CER[NDS] ceramides, confirming an effect of oestrogen on cutaneous ceramide metabolism. Taken together, these data show perturbed stratum corneum lipids post-menopause, and a role for oestrogen in ceramide production.https://doi.org/10.1038/s41598-022-26095-0 |
| spellingShingle | Alexandra C. Kendall Suzanne M. Pilkington Jonathan R. Wray Victoria L. Newton Christopher E. M. Griffiths Mike Bell Rachel E. B. Watson Anna Nicolaou Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy Scientific Reports |
| title | Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy |
| title_full | Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy |
| title_fullStr | Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy |
| title_full_unstemmed | Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy |
| title_short | Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy |
| title_sort | menopause induces changes to the stratum corneum ceramide profile which are prevented by hormone replacement therapy |
| url | https://doi.org/10.1038/s41598-022-26095-0 |
| work_keys_str_mv | AT alexandrackendall menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy AT suzannempilkington menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy AT jonathanrwray menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy AT victorialnewton menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy AT christopheremgriffiths menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy AT mikebell menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy AT rachelebwatson menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy AT annanicolaou menopauseinduceschangestothestratumcorneumceramideprofilewhicharepreventedbyhormonereplacementtherapy |