Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in mice
Objective(s): Immunomodulatory activity of β-glucans of shiitake mushroom (Lentinula edodes) has been known. We investigated whether β-glucans from L. edodes would attenuate the acute effects of lipopolysaccharides (LPS) on peripheral hematological parameters in mice.Materials and Methods: An in-hou...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_22243_e68b88b164709a72258bb61e45e84489.pdf |
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| author | Mozhdeh Jafari Mohammad Hossein Boskabady Seyed Abdolrahim Rezaee Sharareh Rezaeian Sepideh Behrooz Rezvan Ramezannejad Hamid Pourianfar |
| author_facet | Mozhdeh Jafari Mohammad Hossein Boskabady Seyed Abdolrahim Rezaee Sharareh Rezaeian Sepideh Behrooz Rezvan Ramezannejad Hamid Pourianfar |
| author_sort | Mozhdeh Jafari |
| collection | DOAJ |
| description | Objective(s): Immunomodulatory activity of β-glucans of shiitake mushroom (Lentinula edodes) has been known. We investigated whether β-glucans from L. edodes would attenuate the acute effects of lipopolysaccharides (LPS) on peripheral hematological parameters in mice.Materials and Methods: An in-house β-glucans extract (BG) prepared from fruiting bodies of shiitake mushroom L. edodes was chemically measured and characterized using spectrophotometry and HPLC. Male BALB/c mice directly inhaled aerosolized LPS of 3 mg/ml and were treated with BG or commercial β-glucan (known as lentinan; LNT) (10 mg/kg bw) at 1 hr before or 6 hr after LPS inhalation. The blood samples were collected by cardiac puncture from euthanized mice at 16 hr post-treatment. Results: The results showed a significant reduction in levels of blood parameters, including red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), and platelets (PLT); and a significant increase in blood lymphocyte counts in LPS-treated mice as compared with the control mice (P≤0.05). Total white blood cells, neutrophils, and monocyte counts did not show any significant difference among the groups. Treatment of LPS-challenged mice with LNT or BG significantly increased the levels of RBC, HGB, HCT, and PLT; and reduced blood lymphocyte counts as compared with LPS-treated mice (P≤0.05).Conclusion: These findings suggest that β-glucans from L. edodes might be effective in attenuating the effects of inhaled LPS on peripheral blood parameters. Thus, these findings might be useful in acute inflammatory diseases particularly pulmonary infectious diseases in which the hematological parameters would be affected. |
| first_indexed | 2024-03-12T00:04:15Z |
| format | Article |
| id | doaj.art-82e68f9c0a6e490e818bd6fd4f216611 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-03-12T00:04:15Z |
| publishDate | 2023-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-82e68f9c0a6e490e818bd6fd4f2166112023-09-17T06:58:11ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-07-0126783684210.22038/ijbms.2023.67669.1482022243Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in miceMozhdeh Jafari0Mohammad Hossein Boskabady1Seyed Abdolrahim Rezaee2Sharareh Rezaeian3Sepideh Behrooz4Rezvan Ramezannejad5Hamid Pourianfar6School of Medicine, Mashhad University of Medical Sciences, Inflammation and Inflammatory Diseases Research Centre, Mashhad, IranDepartment of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranSchool of Medicine, Mashhad University of Medical Sciences, Inflammation and Inflammatory Diseases Research Centre, Mashhad, IranIndustrial Fungi Biotechnology Research Department, Research Institute for Industrial Biotechnology, Academic Center for Education, Culture and Research (ACECR)- Khorasan Razavi Branch, Mashhad, IranDepartment of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranIndustrial Fungi Biotechnology Research Department, Research Institute for Industrial Biotechnology, Academic Center for Education, Culture and Research (ACECR)- Khorasan Razavi Branch, Mashhad, IranIndustrial Fungi Biotechnology Research Department, Research Institute for Industrial Biotechnology, Academic Center for Education, Culture and Research (ACECR)- Khorasan Razavi Branch, Mashhad, IranObjective(s): Immunomodulatory activity of β-glucans of shiitake mushroom (Lentinula edodes) has been known. We investigated whether β-glucans from L. edodes would attenuate the acute effects of lipopolysaccharides (LPS) on peripheral hematological parameters in mice.Materials and Methods: An in-house β-glucans extract (BG) prepared from fruiting bodies of shiitake mushroom L. edodes was chemically measured and characterized using spectrophotometry and HPLC. Male BALB/c mice directly inhaled aerosolized LPS of 3 mg/ml and were treated with BG or commercial β-glucan (known as lentinan; LNT) (10 mg/kg bw) at 1 hr before or 6 hr after LPS inhalation. The blood samples were collected by cardiac puncture from euthanized mice at 16 hr post-treatment. Results: The results showed a significant reduction in levels of blood parameters, including red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), and platelets (PLT); and a significant increase in blood lymphocyte counts in LPS-treated mice as compared with the control mice (P≤0.05). Total white blood cells, neutrophils, and monocyte counts did not show any significant difference among the groups. Treatment of LPS-challenged mice with LNT or BG significantly increased the levels of RBC, HGB, HCT, and PLT; and reduced blood lymphocyte counts as compared with LPS-treated mice (P≤0.05).Conclusion: These findings suggest that β-glucans from L. edodes might be effective in attenuating the effects of inhaled LPS on peripheral blood parameters. Thus, these findings might be useful in acute inflammatory diseases particularly pulmonary infectious diseases in which the hematological parameters would be affected.https://ijbms.mums.ac.ir/article_22243_e68b88b164709a72258bb61e45e84489.pdfβ-glucanshematological parametersinflammationinhalationlentinan lipopolysaccharideshiitake |
| spellingShingle | Mozhdeh Jafari Mohammad Hossein Boskabady Seyed Abdolrahim Rezaee Sharareh Rezaeian Sepideh Behrooz Rezvan Ramezannejad Hamid Pourianfar Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in mice Iranian Journal of Basic Medical Sciences β-glucans hematological parameters inflammation inhalation lentinan lipopolysaccharide shiitake |
| title | Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in mice |
| title_full | Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in mice |
| title_fullStr | Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in mice |
| title_full_unstemmed | Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in mice |
| title_short | Lentinan and β-glucan extract from shiitake mushroom, Lentinula edodes, alleviate acute LPS-induced hematological changes in mice |
| title_sort | lentinan and β glucan extract from shiitake mushroom lentinula edodes alleviate acute lps induced hematological changes in mice |
| topic | β-glucans hematological parameters inflammation inhalation lentinan lipopolysaccharide shiitake |
| url | https://ijbms.mums.ac.ir/article_22243_e68b88b164709a72258bb61e45e84489.pdf |
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