Genetic variations in the Myostatin gene affecting growth traits in sheep
Background and Aim: Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. Myostatin (MSTN) plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study s...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-02-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.14/February-2021/20.pdf |
| _version_ | 1819122789235294208 |
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| author | Noha M. Osman Heba I. Shafey Mohamed A. Abdelhafez Ahmed M. Sallam Karima F. Mahrous |
| author_facet | Noha M. Osman Heba I. Shafey Mohamed A. Abdelhafez Ahmed M. Sallam Karima F. Mahrous |
| author_sort | Noha M. Osman |
| collection | DOAJ |
| description | Background and Aim: Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. Myostatin (MSTN) plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study sought to investigate genetic variation in the first intron of the MSTN gene and the association of variants with growth traits in major sheep breeds in Egypt (Barki, Ossimi, and Rahmani) and Saudi Arabia (Najdi) using polymerase chain reaction (PCR) and sequencing.
Materials and Methods: Blood samples were collected, and DNA was extracted from 75 animals. A 386 bp fragment in the first intron of the MSTN gene was amplified using PCR. Polymorphic sites were detected using direct sequencing and then correlated with growth traits using a general linear model.
Results: Sequence analysis of the first intron of MSTN gene identified six single-nucleotide polymorphisms (SNPs) in the studied breeds. Four mutual SNPs were determined: c.18 G>T, c.241 T>C, c.243 G>A, and c.259 G>T. In addition, two SNPs c.159 A>T and c.173 T>G were monomorphic (AA and TT, respectively) in the Ossimi, Rahmani, and Najdi breeds and polymorphic in the Barki breed. The association analysis revealed that the c.18 G>T and c.241 C>T significantly associated (p<0.05) with birth weight and average daily weight gain, respectively.
Conclusion: Our results strongly support MSTN as a candidate gene for marker-assisted selection in sheep breeding programs. Furthermore, the identified variants may be considered as putative markers to improve growth traits in sheep. |
| first_indexed | 2024-12-22T06:58:02Z |
| format | Article |
| id | doaj.art-82e99f0920f44f1788e80f8fb4af12b5 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T06:58:02Z |
| publishDate | 2021-02-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-82e99f0920f44f1788e80f8fb4af12b52022-12-21T18:34:52ZengVeterinary WorldVeterinary World0972-89882231-09162021-02-0114247548210.14202/vetworld.2021.475-482Genetic variations in the Myostatin gene affecting growth traits in sheepNoha M. Osman0https://orcid.org/0000-0002-6866-1484Heba I. Shafey1https://orcid.org/0000-0002-2836-029XMohamed A. Abdelhafez2https://orcid.org/0000-0003-3591-5641Ahmed M. Sallam3https://orcid.org/0000-0001-9072-785XKarima F. Mahrous4https://orcid.org/0000-0002-1662-1034Department of Cell Biology, National Research Centre, El Buhouth Street, 12311, Dokki, Egypt.Department of Cell Biology, National Research Centre, El Buhouth Street, 12311, Dokki, Egypt.Department of Cell Biology, National Research Centre, El Buhouth Street, 12311, Dokki, Egypt.Animal and Poultry Production Division, Desert Research Center, 11753, Mataryia, Egypt.Department of Cell Biology, National Research Centre, El Buhouth Street, 12311, Dokki, Egypt.Background and Aim: Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. Myostatin (MSTN) plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study sought to investigate genetic variation in the first intron of the MSTN gene and the association of variants with growth traits in major sheep breeds in Egypt (Barki, Ossimi, and Rahmani) and Saudi Arabia (Najdi) using polymerase chain reaction (PCR) and sequencing. Materials and Methods: Blood samples were collected, and DNA was extracted from 75 animals. A 386 bp fragment in the first intron of the MSTN gene was amplified using PCR. Polymorphic sites were detected using direct sequencing and then correlated with growth traits using a general linear model. Results: Sequence analysis of the first intron of MSTN gene identified six single-nucleotide polymorphisms (SNPs) in the studied breeds. Four mutual SNPs were determined: c.18 G>T, c.241 T>C, c.243 G>A, and c.259 G>T. In addition, two SNPs c.159 A>T and c.173 T>G were monomorphic (AA and TT, respectively) in the Ossimi, Rahmani, and Najdi breeds and polymorphic in the Barki breed. The association analysis revealed that the c.18 G>T and c.241 C>T significantly associated (p<0.05) with birth weight and average daily weight gain, respectively. Conclusion: Our results strongly support MSTN as a candidate gene for marker-assisted selection in sheep breeding programs. Furthermore, the identified variants may be considered as putative markers to improve growth traits in sheep.http://www.veterinaryworld.org/Vol.14/February-2021/20.pdfgrowth traitsmyostatin genepolymorphismsheep |
| spellingShingle | Noha M. Osman Heba I. Shafey Mohamed A. Abdelhafez Ahmed M. Sallam Karima F. Mahrous Genetic variations in the Myostatin gene affecting growth traits in sheep Veterinary World growth traits myostatin gene polymorphism sheep |
| title | Genetic variations in the Myostatin gene affecting growth traits in sheep |
| title_full | Genetic variations in the Myostatin gene affecting growth traits in sheep |
| title_fullStr | Genetic variations in the Myostatin gene affecting growth traits in sheep |
| title_full_unstemmed | Genetic variations in the Myostatin gene affecting growth traits in sheep |
| title_short | Genetic variations in the Myostatin gene affecting growth traits in sheep |
| title_sort | genetic variations in the myostatin gene affecting growth traits in sheep |
| topic | growth traits myostatin gene polymorphism sheep |
| url | http://www.veterinaryworld.org/Vol.14/February-2021/20.pdf |
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