Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies.
Both the Mediterranean (MED) species of the Bemisia tabaci whitefly complex and the greenhouse whitefly (Trialeurodes vaporariorum, TV) are important agricultural pests. The two species of whiteflies differ in many aspects such as morphology, geographical distribution, host plant range, plant virus...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0237744 |
| _version_ | 1819147709630644224 |
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| author | Yu-Jun Wang Hua-Ling Wang Xiao-Wei Wang Shu-Sheng Liu |
| author_facet | Yu-Jun Wang Hua-Ling Wang Xiao-Wei Wang Shu-Sheng Liu |
| author_sort | Yu-Jun Wang |
| collection | DOAJ |
| description | Both the Mediterranean (MED) species of the Bemisia tabaci whitefly complex and the greenhouse whitefly (Trialeurodes vaporariorum, TV) are important agricultural pests. The two species of whiteflies differ in many aspects such as morphology, geographical distribution, host plant range, plant virus transmission, and resistance to insecticides. However, the molecular basis underlying their differences remains largely unknown. In this study, we analyzed the genetic divergences between the transcriptomes of MED and TV. In total, 2,944 pairs of orthologous genes were identified. The average identity of amino acid sequences between the two species is 93.6%. The average nonsynonymous (Ka) and synonymous (Ks) substitution rates and the ratio of Ka/Ks of the orthologous genes are 0.0389, 2.23 and 0.0204, respectively. The low average Ka/Ks ratio indicates that orthologous genes tend to be under strong purified selection. The most divergent gene classes are related to the metabolisms of xenobiotics, cofactors, vitamins and amino acids, and this divergence may underlie the different biological characteristics between the two species of whiteflies. Genes of differential expression between the two species are enriched in carbohydrate metabolism and regulation of autophagy. These findings provide molecular clues to uncover the biological and molecular differences between the two species of whiteflies. |
| first_indexed | 2024-12-22T13:34:08Z |
| format | Article |
| id | doaj.art-82ea5fd1cc5d4764b7b0c4ebff4c4bf6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:34:08Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-82ea5fd1cc5d4764b7b0c4ebff4c4bf62022-12-21T18:24:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01158e023774410.1371/journal.pone.0237744Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies.Yu-Jun WangHua-Ling WangXiao-Wei WangShu-Sheng LiuBoth the Mediterranean (MED) species of the Bemisia tabaci whitefly complex and the greenhouse whitefly (Trialeurodes vaporariorum, TV) are important agricultural pests. The two species of whiteflies differ in many aspects such as morphology, geographical distribution, host plant range, plant virus transmission, and resistance to insecticides. However, the molecular basis underlying their differences remains largely unknown. In this study, we analyzed the genetic divergences between the transcriptomes of MED and TV. In total, 2,944 pairs of orthologous genes were identified. The average identity of amino acid sequences between the two species is 93.6%. The average nonsynonymous (Ka) and synonymous (Ks) substitution rates and the ratio of Ka/Ks of the orthologous genes are 0.0389, 2.23 and 0.0204, respectively. The low average Ka/Ks ratio indicates that orthologous genes tend to be under strong purified selection. The most divergent gene classes are related to the metabolisms of xenobiotics, cofactors, vitamins and amino acids, and this divergence may underlie the different biological characteristics between the two species of whiteflies. Genes of differential expression between the two species are enriched in carbohydrate metabolism and regulation of autophagy. These findings provide molecular clues to uncover the biological and molecular differences between the two species of whiteflies.https://doi.org/10.1371/journal.pone.0237744 |
| spellingShingle | Yu-Jun Wang Hua-Ling Wang Xiao-Wei Wang Shu-Sheng Liu Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies. PLoS ONE |
| title | Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies. |
| title_full | Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies. |
| title_fullStr | Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies. |
| title_full_unstemmed | Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies. |
| title_short | Transcriptome analysis and comparison reveal divergence between the Mediterranean and the greenhouse whiteflies. |
| title_sort | transcriptome analysis and comparison reveal divergence between the mediterranean and the greenhouse whiteflies |
| url | https://doi.org/10.1371/journal.pone.0237744 |
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