MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo
Background MicroRNA-154-5p (miR-154-5p) plays a role in tumorigenesis in diverse human malignancies. Nevertheless, little is known about the mechanism by which miR-154-5p alters the growth and metastasis of cervical cancer. This research aimed to analyze the role of miR-154-5p in the pathology of ce...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2023-06-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/15641.pdf |
| _version_ | 1797424055498833920 |
|---|---|
| author | Yaqin Li Yimiao Wei Honglei Zhang Ying Bai Xiuting Wang Qi Li Yatao Liu Shuling Wang Jiapu Wang Songquan Wen Jiarong Li Weihong Zhao |
| author_facet | Yaqin Li Yimiao Wei Honglei Zhang Ying Bai Xiuting Wang Qi Li Yatao Liu Shuling Wang Jiapu Wang Songquan Wen Jiarong Li Weihong Zhao |
| author_sort | Yaqin Li |
| collection | DOAJ |
| description | Background MicroRNA-154-5p (miR-154-5p) plays a role in tumorigenesis in diverse human malignancies. Nevertheless, little is known about the mechanism by which miR-154-5p alters the growth and metastasis of cervical cancer. This research aimed to analyze the role of miR-154-5p in the pathology of cervical cancer in vitro and in vivo. Methods The level of miR-154-5p in human papillomavirus 16 positive cervical cancer cells was examined by real-time quantitative polymerase chain reaction. Bioinformatics predicted the downstream targets and potential functions of miR-154-5p. Furthermore, lentiviral technology was used to construct SiHa cell lines with stable up- and down-expression levels of miR-154-5p. Its differential expression effects on the progress and metastasis of cervical cancer were analyzed using cell culture and animal models. Results MiR-154-5p showed low expression in cervical cancer cells. Overexpression of miR-154-5p could markedly inhibit the proliferation, migration, and colony formation ability of SiHa cells, concomitantly leading to G1 arrest of the cell cycle, while silencing miR-154-5p triggered the opposite results. Meanwhile, overexpression of miR-154-5p restrained the growth and metastasis of cervical cancer by silencing CUL2 in vivo. Additionally, miR-154-5p reduced CUL2 level, and overexpression of CUL2 influenced the effect of miR-154-5p in cervical cancer. In conclusion, miR-154-5p restrained the growth and metastasis of cervical cancer by directly silencing CUL2. |
| first_indexed | 2024-03-09T07:56:45Z |
| format | Article |
| id | doaj.art-82ead1d22dcf41a9922774bd1ffdcc28 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:56:45Z |
| publishDate | 2023-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-82ead1d22dcf41a9922774bd1ffdcc282023-12-03T00:58:57ZengPeerJ Inc.PeerJ2167-83592023-06-0111e1564110.7717/peerj.15641MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivoYaqin Li0Yimiao Wei1Honglei Zhang2Ying Bai3Xiuting Wang4Qi Li5Yatao Liu6Shuling Wang7Jiapu Wang8Songquan Wen9Jiarong Li10Weihong Zhao11Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Pathology and Pathophysiology,Basic Medical College, Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Biochemistry and Molecular Biology,Basic Medical College, Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Epidemiology,School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province, ChinaScientific Research Experiment Center, Central laboratory, The Affiliated Cardiovascular Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Epidemiology,School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, ChinaBackground MicroRNA-154-5p (miR-154-5p) plays a role in tumorigenesis in diverse human malignancies. Nevertheless, little is known about the mechanism by which miR-154-5p alters the growth and metastasis of cervical cancer. This research aimed to analyze the role of miR-154-5p in the pathology of cervical cancer in vitro and in vivo. Methods The level of miR-154-5p in human papillomavirus 16 positive cervical cancer cells was examined by real-time quantitative polymerase chain reaction. Bioinformatics predicted the downstream targets and potential functions of miR-154-5p. Furthermore, lentiviral technology was used to construct SiHa cell lines with stable up- and down-expression levels of miR-154-5p. Its differential expression effects on the progress and metastasis of cervical cancer were analyzed using cell culture and animal models. Results MiR-154-5p showed low expression in cervical cancer cells. Overexpression of miR-154-5p could markedly inhibit the proliferation, migration, and colony formation ability of SiHa cells, concomitantly leading to G1 arrest of the cell cycle, while silencing miR-154-5p triggered the opposite results. Meanwhile, overexpression of miR-154-5p restrained the growth and metastasis of cervical cancer by silencing CUL2 in vivo. Additionally, miR-154-5p reduced CUL2 level, and overexpression of CUL2 influenced the effect of miR-154-5p in cervical cancer. In conclusion, miR-154-5p restrained the growth and metastasis of cervical cancer by directly silencing CUL2.https://peerj.com/articles/15641.pdfCervical cancerMicroRNA-154-5pCullin2GrowthMetastasis |
| spellingShingle | Yaqin Li Yimiao Wei Honglei Zhang Ying Bai Xiuting Wang Qi Li Yatao Liu Shuling Wang Jiapu Wang Songquan Wen Jiarong Li Weihong Zhao MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo PeerJ Cervical cancer MicroRNA-154-5p Cullin2 Growth Metastasis |
| title | MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo |
| title_full | MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo |
| title_fullStr | MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo |
| title_full_unstemmed | MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo |
| title_short | MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo |
| title_sort | microrna 154 5p suppresses cervical carcinoma growth and metastasis by silencing cullin2 in vitro and in vivo |
| topic | Cervical cancer MicroRNA-154-5p Cullin2 Growth Metastasis |
| url | https://peerj.com/articles/15641.pdf |
| work_keys_str_mv | AT yaqinli microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT yimiaowei microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT hongleizhang microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT yingbai microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT xiutingwang microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT qili microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT yataoliu microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT shulingwang microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT jiapuwang microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT songquanwen microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT jiarongli microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo AT weihongzhao microrna1545psuppressescervicalcarcinomagrowthandmetastasisbysilencingcullin2invitroandinvivo |