Diuretics prime plant immunity in Arabidopsis thaliana.
Plant activators are agrochemicals that activate the plant immune system, thereby enhancing disease resistance. Due to their prophylactic and durable effects on a wide spectrum of diseases, plant activators can provide synergistic crop protection when used in combination with traditional pest contro...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144763/?tool=EBI |
| _version_ | 1818742587697135616 |
|---|---|
| author | Yoshiteru Noutoshi Mika Ikeda Ken Shirasu |
| author_facet | Yoshiteru Noutoshi Mika Ikeda Ken Shirasu |
| author_sort | Yoshiteru Noutoshi |
| collection | DOAJ |
| description | Plant activators are agrochemicals that activate the plant immune system, thereby enhancing disease resistance. Due to their prophylactic and durable effects on a wide spectrum of diseases, plant activators can provide synergistic crop protection when used in combination with traditional pest controls. Although plant activators have achieved great success in wet-rice farming practices in Asia, their use is still limited. To isolate novel plant activators applicable to other crops, we screened a chemical library using a method that can selectively identify immune-priming compounds. Here, we report the isolation and characterization of three diuretics, bumetanide, bendroflumethiazide and clopamide, as immune-priming compounds. These drugs upregulate the immunity-related cell death of Arabidopsis suspension-cultured cells induced with an avirulent strain of Pseudomonas syringae pv. tomato in a concentration-dependent manner. The application of these compounds to Arabidopsis plants confers disease resistance to not only the avirulent but also a virulent strain of the pathogen. Unlike salicylic acid, an endogenous phytohormone that governs disease resistance in response to biotrophic pathogens, the three diuretic compounds analyzed here do not induce PR1 or inhibit plant growth, showing potential as lead compounds in a practical application. |
| first_indexed | 2024-12-18T02:14:54Z |
| format | Article |
| id | doaj.art-82eb141abd1547d1957022bcb947b3b5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T02:14:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-82eb141abd1547d1957022bcb947b3b52022-12-21T21:24:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4844310.1371/journal.pone.0048443Diuretics prime plant immunity in Arabidopsis thaliana.Yoshiteru NoutoshiMika IkedaKen ShirasuPlant activators are agrochemicals that activate the plant immune system, thereby enhancing disease resistance. Due to their prophylactic and durable effects on a wide spectrum of diseases, plant activators can provide synergistic crop protection when used in combination with traditional pest controls. Although plant activators have achieved great success in wet-rice farming practices in Asia, their use is still limited. To isolate novel plant activators applicable to other crops, we screened a chemical library using a method that can selectively identify immune-priming compounds. Here, we report the isolation and characterization of three diuretics, bumetanide, bendroflumethiazide and clopamide, as immune-priming compounds. These drugs upregulate the immunity-related cell death of Arabidopsis suspension-cultured cells induced with an avirulent strain of Pseudomonas syringae pv. tomato in a concentration-dependent manner. The application of these compounds to Arabidopsis plants confers disease resistance to not only the avirulent but also a virulent strain of the pathogen. Unlike salicylic acid, an endogenous phytohormone that governs disease resistance in response to biotrophic pathogens, the three diuretic compounds analyzed here do not induce PR1 or inhibit plant growth, showing potential as lead compounds in a practical application.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144763/?tool=EBI |
| spellingShingle | Yoshiteru Noutoshi Mika Ikeda Ken Shirasu Diuretics prime plant immunity in Arabidopsis thaliana. PLoS ONE |
| title | Diuretics prime plant immunity in Arabidopsis thaliana. |
| title_full | Diuretics prime plant immunity in Arabidopsis thaliana. |
| title_fullStr | Diuretics prime plant immunity in Arabidopsis thaliana. |
| title_full_unstemmed | Diuretics prime plant immunity in Arabidopsis thaliana. |
| title_short | Diuretics prime plant immunity in Arabidopsis thaliana. |
| title_sort | diuretics prime plant immunity in arabidopsis thaliana |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144763/?tool=EBI |
| work_keys_str_mv | AT yoshiterunoutoshi diureticsprimeplantimmunityinarabidopsisthaliana AT mikaikeda diureticsprimeplantimmunityinarabidopsisthaliana AT kenshirasu diureticsprimeplantimmunityinarabidopsisthaliana |