GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean
Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by the oomycete Phytophthora sojae, is a destructive disease worldwide. The molecular mechanism of the soybean response to P. sojae is largely unclear. We report a novel WRKY transcription factor (TF) in soybean, GmWRKY31, in...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00781/full |
| _version_ | 1818607693846282240 |
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| author | Sujie Fan Sujie Fan Lidong Dong Dan Han Feng Zhang Junjiang Wu Liangyu Jiang Liangyu Jiang Qun Cheng Rongpeng Li Wencheng Lu Fanshan Meng Shuzhen Zhang Pengfei Xu |
| author_facet | Sujie Fan Sujie Fan Lidong Dong Dan Han Feng Zhang Junjiang Wu Liangyu Jiang Liangyu Jiang Qun Cheng Rongpeng Li Wencheng Lu Fanshan Meng Shuzhen Zhang Pengfei Xu |
| author_sort | Sujie Fan |
| collection | DOAJ |
| description | Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by the oomycete Phytophthora sojae, is a destructive disease worldwide. The molecular mechanism of the soybean response to P. sojae is largely unclear. We report a novel WRKY transcription factor (TF) in soybean, GmWRKY31, in the host response to P. sojae. Overexpression and RNA interference analysis demonstrated that GmWRKY31 enhanced resistance to P. sojae in transgenic soybean plants. GmWRKY31 was targeted to the nucleus, where it bound to the W-box and acted as an activator of gene transcription. Moreover, we determined that GmWRKY31 physically interacted with GmHDL56, which improved resistance to P. sojae in transgenic soybean roots. GmWRKY31 and GmHDL56 shared a common target GmNPR1 which was induced by P. sojae. Overexpression and RNA interference analysis demonstrated that GmNPR1 enhanced resistance to P. sojae in transgenic soybean plants. Several pathogenesis-related (PR) genes were constitutively activated, including GmPR1a, GmPR2, GmPR3, GmPR4, GmPR5a, and GmPR10, in soybean plants overexpressing GmNPR1 transcripts. By contrast, the induction of PR genes was compromised in transgenic GmNPR1-RNAi lines. Taken together, these findings suggested that the interaction between GmWRKY31 and GmHDL56 enhances resistance to P. sojae by regulating defense-related gene expression in soybean. |
| first_indexed | 2024-12-16T14:30:49Z |
| format | Article |
| id | doaj.art-82ebb5668b574a538c99f6a8fd32c32d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T14:30:49Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-82ebb5668b574a538c99f6a8fd32c32d2022-12-21T22:28:15ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00781262214GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in SoybeanSujie Fan0Sujie Fan1Lidong Dong2Dan Han3Feng Zhang4Junjiang Wu5Liangyu Jiang6Liangyu Jiang7Qun Cheng8Rongpeng Li9Wencheng Lu10Fanshan Meng11Shuzhen Zhang12Pengfei Xu13Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaCenter for Plant Biotechnology, College of Agronomy, Jilin Agricultural UniversityChangchun, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaFirst Affiliated Hospital of Harbin Medical UniversityHarbin, ChinaSoybean Research Institute, Key Laboratory of Soybean Cultivation of Ministry of Agriculture, Heilongjiang Academy of Agricultural SciencesHarbin, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaCenter for Plant Biotechnology, College of Agronomy, Jilin Agricultural UniversityChangchun, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaHeihe Branch of Heilongjiang Academy of Agricultural SciencesHeihe, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaSoybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural UniversityHarbin, ChinaPhytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by the oomycete Phytophthora sojae, is a destructive disease worldwide. The molecular mechanism of the soybean response to P. sojae is largely unclear. We report a novel WRKY transcription factor (TF) in soybean, GmWRKY31, in the host response to P. sojae. Overexpression and RNA interference analysis demonstrated that GmWRKY31 enhanced resistance to P. sojae in transgenic soybean plants. GmWRKY31 was targeted to the nucleus, where it bound to the W-box and acted as an activator of gene transcription. Moreover, we determined that GmWRKY31 physically interacted with GmHDL56, which improved resistance to P. sojae in transgenic soybean roots. GmWRKY31 and GmHDL56 shared a common target GmNPR1 which was induced by P. sojae. Overexpression and RNA interference analysis demonstrated that GmNPR1 enhanced resistance to P. sojae in transgenic soybean plants. Several pathogenesis-related (PR) genes were constitutively activated, including GmPR1a, GmPR2, GmPR3, GmPR4, GmPR5a, and GmPR10, in soybean plants overexpressing GmNPR1 transcripts. By contrast, the induction of PR genes was compromised in transgenic GmNPR1-RNAi lines. Taken together, these findings suggested that the interaction between GmWRKY31 and GmHDL56 enhances resistance to P. sojae by regulating defense-related gene expression in soybean.http://journal.frontiersin.org/article/10.3389/fpls.2017.00781/fullGlycine maxGmWRKY31GmHDL56Phytophthora sojaeresponse selection |
| spellingShingle | Sujie Fan Sujie Fan Lidong Dong Dan Han Feng Zhang Junjiang Wu Liangyu Jiang Liangyu Jiang Qun Cheng Rongpeng Li Wencheng Lu Fanshan Meng Shuzhen Zhang Pengfei Xu GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean Frontiers in Plant Science Glycine max GmWRKY31 GmHDL56 Phytophthora sojae response selection |
| title | GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean |
| title_full | GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean |
| title_fullStr | GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean |
| title_full_unstemmed | GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean |
| title_short | GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean |
| title_sort | gmwrky31 and gmhdl56 enhances resistance to phytophthora sojae by regulating defense related gene expression in soybean |
| topic | Glycine max GmWRKY31 GmHDL56 Phytophthora sojae response selection |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00781/full |
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