Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i>
<i>Impatiens walleriana</i> is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted <i>I. walleriana</i> plants from drought stress. The plants were divided into: watered plants, dr...
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MDPI AG
2020-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/11/1589 |
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| author | Dragana D. Antonić Angelina R. Subotić Milan B. Dragićević Danijel Pantelić Snežana M. Milošević Ana D. Simonović Ivana Momčilović |
| author_facet | Dragana D. Antonić Angelina R. Subotić Milan B. Dragićević Danijel Pantelić Snežana M. Milošević Ana D. Simonović Ivana Momčilović |
| author_sort | Dragana D. Antonić |
| collection | DOAJ |
| description | <i>Impatiens walleriana</i> is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted <i>I. walleriana</i> plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: <i>IwDhn1, IwDhn2.1</i> and <i>IwDhn2.2.</i> Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, <i>Impatiens</i> accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of <i>IwDhn2.1</i> and <i>IwDhn2.2.</i> Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation. |
| first_indexed | 2024-03-10T14:47:52Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:47:52Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-82edd85297644f9a92ff60363555eb892023-11-20T21:13:06ZengMDPI AGPlants2223-77472020-11-01911158910.3390/plants9111589Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i>Dragana D. Antonić0Angelina R. Subotić1Milan B. Dragićević2Danijel Pantelić3Snežana M. Milošević4Ana D. Simonović5Ivana Momčilović6Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia<i>Impatiens walleriana</i> is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted <i>I. walleriana</i> plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: <i>IwDhn1, IwDhn2.1</i> and <i>IwDhn2.2.</i> Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, <i>Impatiens</i> accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of <i>IwDhn2.1</i> and <i>IwDhn2.2.</i> Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.https://www.mdpi.com/2223-7747/9/11/1589antioxidative enzymescatalasefloweringmalondialdehydeperoxidaseproline |
| spellingShingle | Dragana D. Antonić Angelina R. Subotić Milan B. Dragićević Danijel Pantelić Snežana M. Milošević Ana D. Simonović Ivana Momčilović Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i> Plants antioxidative enzymes catalase flowering malondialdehyde peroxidase proline |
| title | Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i> |
| title_full | Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i> |
| title_fullStr | Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i> |
| title_full_unstemmed | Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i> |
| title_short | Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in <i>Impatiens walleriana</i> |
| title_sort | effects of exogenous salicylic acid on drought response and characterization of dehydrins in i impatiens walleriana i |
| topic | antioxidative enzymes catalase flowering malondialdehyde peroxidase proline |
| url | https://www.mdpi.com/2223-7747/9/11/1589 |
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